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Identification of Cancer Stem Cell and its niche system in Oral Cancer

Research Project

Project/Area Number 21390539
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHiroshima University

Principal Investigator

OKAMOTO Tetsuji  広島大学, 大学院・医歯薬学総合研究科, 教授 (00169153)

Co-Investigator(Kenkyū-buntansha) TORATANI Shigeaki  広島大学, 病院, 講師 (90172220)
HAYASHIDOU Yasutaka  広島大学, 病院, 講師 (70243251)
SHINTANI Tomoaki  広島大学, 病院, 助教 (90403518)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords口腔癌 / 癌幹細胞 / 無血清培地 / CD133 / Sp細胞 / 低酸素 / sphere形成 / 造腫瘍性 / 口腔扁平上皮癌細胞 / side population / hoechst33342 / 無血清培養 / HIF-1遺伝子 / 薬剤耐性遺伝子 / 低酸素培養 / CD133陽性細胞 / sphere形成能 / 低酸素環境 / 抗癌剤 / 細胞増殖因子
Research Abstract

We have revealed that CD133-positive cells in oral squamous cell carcinoma cells has an important role for the sphere formation and side population cells also function for tumorigenicity and drug-resistance activity. In addition, epidermal growth factor, sonic hedgehog, interleukin-6 derived from the OSCC work as niche for the maintenance and property of CD133 cells. These results strongly suggested the possibility that CD133 and/ or Sp cells function as a cancer stem cell in oral squamous cell carcinomas. Thus, targeted therapy against the CD133 and/ or Sp cell might be useful for the OSCC therapy

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (48 results)

All 2012 2011 2010 2009 Other

All Journal Article (23 results) (of which Peer Reviewed: 15 results) Presentation (18 results) Book (2 results) Patent(Industrial Property Rights) (5 results)

  • [Journal Article] Novel cytotoxic polyoxygenated steroids from an Okinawan Sponge, Dysidea sp. Sudhakar2012

    • Author(s)
      V. S., Yukio Yoshioka, Tetsuji Okamoto, Makoto Ojika
    • Journal Title

      Makoto Ojika, Biosci. Biotechnol. Biochem

      Volume: 76(5) Pages: 999-1002

    • Related Report
      2011 Final Research Report
  • [Journal Article] a Novel PrenylatedGermacrene Diterpene, from the Soft CoralLobophytum pauciflorum, Sudhakar2012

    • Author(s)
      Cyclolobatriene
    • Journal Title

      Bioorganic & Medicinal Chemistry, Jan

      Volume: 20(2) Pages: 687-92

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cyclolobatriene, a novel prenylated germacrene, from the soft coral Lobophytum pauciflorum2012

    • Author(s)
      Govindam SV, Yoshioka Y, Kanamoto A, Fujiwara T, Okamoto T, Ojika M
    • Journal Title

      Bioorg Med Chem

      Volume: 15;20(2) Pages: 687-692

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prospect of mouse embryonic stemcell-derived neural crest cells to in vitroassay for develop-mental toxicology2011

    • Author(s)
      Mimura, S., Okamoto, T., Furue-Kusuda, M., et al
    • Journal Title

      Tissue Culture ResearchCommunications

      Volume: Vol.30, No.2-4 Pages: 159-168

    • Related Report
      2011 Final Research Report
  • [Journal Article] FGFR1 abrogates inhibitory effect of androgen receptor concurrent with induction of androgen-receptor variants in androgen receptor-negative prostate tumor epithelial cells2011

    • Author(s)
      Kobayashi M, Okamoto, T
    • Journal Title

      McKeehan WL. et al., Prostate

      Volume: 71(15) Pages: 1691-1700

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Growth factor-defined culture medium for human mesenchymal stem cells2011

    • Author(s)
      Mimura S, Kimura N, Hirata M, Tateyama D, Hayashida M, Umezawa A, Kohara A, Nikawa H, Okamoto T
    • Journal Title

      Furue MK. Int JDev Biol

      Volume: 55(2) Pages: 181-187

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] GFR1 abrogates inhibitory effect of androgen receptor concurrent with induction of androgen-receptor variants in androgen receptor-negative prostate tumor epithelial cells2011

    • Author(s)
      Kobayashi M, Huang Y, Jin C, Luo Y, Okamoto, T, Wang F, McKeehan WL
    • Journal Title

      Prostate. : 2011

      Volume: Nov;71(15) Pages: 1691-1700

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth factor-defined culture medium for human mesenchymal stem cells2011

    • Author(s)
      Mimura S, Kimura N, Okamoto, T, et al.
    • Journal Title

      Int J Dev Biol

      Volume: 55(2) Pages: 181-187

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FGFR1 abrogates inhibitory effect of androgen receptor concurrent with induction of androgen-receptor variants in androgen receptor-negative prostate tumor epithelial cells.2011

    • Author(s)
      Kobayashi M, Huang Y, Jin C, Luo Y, Okamoto T, Wang F, McKeehan WL.
    • Journal Title

      Prostate, doi:10.1002/pros.21386.

      Volume: Mar 28(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth factor-defined culture medium for human mesenchymal stem cells.2011

    • Author(s)
      Mimura S, Kimura N, Okamoto T, et al.
    • Journal Title

      Int J Dev Biol.

      Volume: Feb 3.(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Influence Of The Lop Nor Nuclear Weapons Test Base To The Population Of The Republic Of Kazakhstan2011

    • Author(s)
      Zhumadilov K., Okamoto, T., Hoshi-M., et al.
    • Journal Title

      Radiation Measurements.

      Volume: 46 Pages: 425-429

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tissue culture : the unlimited potential2010

    • Author(s)
      SatoGH, Sato JD, Okamoto T, McKeehan WL, Barnes DW
    • Journal Title

      In Vitro Cell Dev Biol Anim. 2010 Jul

      Volume: 46(7) Pages: 590-4

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium. Furue2010

    • Author(s)
      MK, Na J, Okamoto T, Sato JD
    • Journal Title

      InVitro Cell Dev Biol Anim

      Volume: 46(7) Pages: 573-6

    • Related Report
      2011 Final Research Report
  • [Journal Article] Binding of APC and disheveled mediated Wnt5a-regulated focal adhesion dynamics in migrating cells2010

    • Author(s)
      Matsumoto S, Fumoto K, Okamoto T, Kaibuchi K, Kikuchi A
    • Journal Title

      EMBOJ

      Volume: 29(7) Pages: 1192-204

    • Related Report
      2011 Final Research Report
  • [Journal Article] Tissue culture : the unlimited potential.2010

    • Author(s)
      Sato GH, Sato JD, Okamoto T, McKeehan WL, Barnes DW.
    • Journal Title

      In Vitro Cell Dev Biol Anim.

      Volume: Jul;46(7) Pages: 590-594

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Advantages and difficulties in culturing human pluripotent stem cells in growth factor-defined serum-free medium.2010

    • Author(s)
      Kusuda Furue M, Tateyama D, Okamoto T, Sato JD., et al.
    • Journal Title

      In Vitro Cell Dev Biol Anim.

      Volume: Jul;46(7) Pages: 573-576

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Binding of APC and dishevelled mediates Wnt5a-regulated focal adhesion dynamics in migrating cells2010

    • Author(s)
      Matsumoto, S., et al
    • Journal Title

      The EMBO Journal 29

      Pages: 1192-1204

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] B-Catenin-independent Wnt signaling regulates cell migration, polarity, and morphogenesis2009

    • Author(s)
      Matsumoto, S
    • Journal Title

      Tissue Culture Research Communications Vol

      Volume: 28 Pages: 117-128

    • Related Report
      2011 Final Research Report
  • [Journal Article] ESR dosimetry study on population of settlements nearby Ust-Kamenogorsk city, Kazakhstan2009

    • Author(s)
      Zhumadilov K
    • Journal Title

      RadiatEnviron Biophys

      Volume: 48(4) Pages: 419-425

    • Related Report
      2011 Final Research Report
  • [Journal Article] B-Catenin-independent Wnt signaling regulates cell migration, polarity, and morphogenesis2009

    • Author(s)
      Matsumoto, S., et al.
    • Journal Title

      Tissue Culture Research Communications Vol.28

      Pages: 117-128

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ESR dosimetry study on population of settlements nearby Ust-Kamenogorsk city, Kazakhstan2009

    • Author(s)
      Zhumadilov K, et al.
    • Journal Title

      Radiat Environ Biophys. Nov:48(4)

      Pages: 419-425

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ヒト人工多能性幹(iPS)細胞の単層無血清培養系の確立

    • Author(s)
      嶋本顕/木村直大/田原栄俊/岡本哲治
    • Journal Title

      日本口腔組織培養学会誌

      Volume: 第21巻第1号 Pages: 31-32

    • Related Report
      2011 Final Research Report
  • [Journal Article] マウス人工多能性幹(iPS)細胞の単層無血清培養系の確立

    • Author(s)
      山崎鍋島巧、木村直大、古江美保、岡本哲治
    • Journal Title

      日本口腔組織培養学会誌

      Volume: 第20巻第1号 Pages: 39-40

    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト人工多能性幹(iPS)細胞の単層無血清培養系の確立2011

    • Author(s)
      嶋本顕/木村直大/田原栄俊/岡本哲治
    • Organizer
      第48回日本口腔組織培養学会
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 口腔扁平上皮癌におけるSP細胞群の癌幹細胞としての分子・生物学的特性の検討2011

    • Author(s)
      伊藤翼, 藤井良典, 石田康隆, Choon Yee Fan, 岡本哲治
    • Organizer
      第65回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      東京都、タワーホール船堀
    • Year and Date
      2011-04-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 無血清浮遊培養系を用いたCD133陽性口腔扁平上皮癌細胞のsphere形成能とその癌幹細胞としての細胞・分子生物学的特性の検討2011

    • Author(s)
      藤井良典, 伊藤翼, 石田康隆, Choon YeeFan, 岡本哲治
    • Organizer
      第65回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      東京都、タワーホール船堀
    • Year and Date
      2011-04-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of Medium Conditoned by hMSCs on Biological Property of CSCs from OSCC Cell Lines in Serum-free Culture、Choon Yee Fan2011

    • Author(s)
      Choon Yee Fan, 石田康隆, 藤井良典, 木村直大, 岡本哲治
    • Organizer
      第65回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      東京都、タワーホール船堀
    • Year and Date
      2011-04-21
    • Related Report
      2011 Final Research Report
  • [Presentation] Long term serial cultivation of mouse induced pluripotent stem cells in serum free-and feeder-free growth factor defined culture2011

    • Author(s)
      T. Okamoto
    • Organizer
      Annual Meeting of Association for Dental Sciencof the Republic of China
    • Place of Presentation
      Taichung
    • Related Report
      2011 Final Research Report
  • [Presentation] 2011 In Vitro Biology Meeting、Long-termserial cultivation of mouse inducedpluripotent stem(iPS) cells in serum-freeand feeder-free defined medium2011

    • Author(s)
      Sachiko Yamasaki, Miho Furue, J. DenrySato, Tetsuji Okamoto
    • Organizer
      In Vitro Cellular & Developmental Biology-Animal Volume 47
    • Place of Presentation
      USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Long term serial cultivation of mouse induced pluripotent stem cells in serum free- and feeder-free growth factor defined culture2011

    • Author(s)
      Tetsuji Okamoto
    • Organizer
      Annual Meeting of Association for Dental Scienc of the Republic of China, Taichung
    • Place of Presentation
      Taichung, Taiwan(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 無血清培養系を用いたヒト口腔扁平上皮癌細胞株由来CD133陽性細胞群の細胞生物学的特性の検討2010

    • Author(s)
      藤井良典, 伊藤翼, 石田康隆, 松本真司, Choon Yee Fan, 岡本哲治
    • Organizer
      日本口腔組織培養学会学術大会
    • Place of Presentation
      高知市、高知県教育会館高知城ホール4F多目的ホール
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 無血清培養系を用いたヒト口腔扁平上皮癌細胞株由来CD133陽性細胞群の細胞・分子生物学的特性の検討2010

    • Author(s)
      藤井良典, 石田康隆, 松本真司, Choon Yee Fan, 岡本哲治
    • Organizer
      第64回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      札幌市、札幌プリンスホテル国際館パミール
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of Medium Conditoned by hMSCs on growth, motility and sphere formation of SCC cell lines in Serum-free Culture2010

    • Author(s)
      ChoonYee Fan, 石田康隆, 藤井良典, , 岡本哲治
    • Organizer
      第64回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      札幌市、札幌プリンスホテル国際館パミール
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Cellular and molecular characteristics of CD133-positive cells derived from oral squamous cell carcinoma cell line in serum-free defined culture」Fujii Y2010

    • Author(s)
      IshidaY, Matsumoto S, Choon Y, Okamoto, T
    • Organizer
      The International Workshop on BioDental Education and Research2010
    • Place of Presentation
      広島市、アステールプラザ広島
    • Year and Date
      2010-02-12
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス人工多能性幹(iPS)細胞の単層無血清培養系の確立2010

    • Author(s)
      山崎鍋島巧/木村直大/古江美保/岡本哲治
    • Organizer
      第47回日本口腔組織培養学会
    • Place of Presentation
      高知、高知城ホール
    • Related Report
      2011 Final Research Report
  • [Presentation] Neuroectodermal Differentiation from embryonic stem cells in Xenopus, Mouse, and Human in serum-free defined culture2009

    • Author(s)
      Okamoto, T
    • Organizer
      The 32nd Annual Scientific Meeting of Association for Dental Sciences of the Republic of China(ADS-ROC)
    • Place of Presentation
      Kaohsiung, Taiwan
    • Year and Date
      2009-11-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Neuroectodermal Differentiation from embryonic stem cells in Xenopus, Mouse, and Human in serum-free defined culture2009

    • Author(s)
      Okamoto, T.
    • Organizer
      The 32nd Annual Scientific Meeting of Association for Dental Sciences of the Republic of China (ADS-ROC)
    • Place of Presentation
      Kaohsiung, Taiwan
    • Year and Date
      2009-11-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 無血清浮遊培養系を用いた口腔扁平上皮癌細胞のsphere形成とその癌幹細胞としての細胞・分子生物学的特性2009

    • Author(s)
      藤井良典, 石田康隆, 松本真司, 岡本哲治
    • Organizer
      第63回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      浜松市、アクトシティ浜松
    • Year and Date
      2009-04-17
    • Related Report
      2011 Final Research Report
  • [Presentation] ヒト人工多能性幹(iPS)細胞の単層無血清培養系の確立

    • Author(s)
      嶋本顕/木村直大/田原栄俊/古江美保/岡本哲治
    • Organizer
      第65回NPO法人日本口腔科学会学術集会
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス人工多能性幹(iPS)細胞を用いた頭部神経系の分化誘導

    • Author(s)
      鍋島巧, 山崎佐知子, 木村直大, 楠田美保, 岡本哲治
    • Organizer
      第65回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      江戸川区,タワーホール船堀
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス人工多能性幹細胞(iPS)細胞の未分化性と多分化能を維持した単層無血清培養系の確立

    • Author(s)
      鍋島巧, 木村直大, 楠田美保, 岡本哲治
    • Organizer
      第64回特定非営利活動法人日本口腔科学会学術集会
    • Place of Presentation
      札幌市,札幌プリンスホテル国際館パミール
    • Related Report
      2011 Final Research Report
  • [Book] 口腔外科学2010

    • Author(s)
      白砂兼光,古郷幹彦,他
    • Total Pages
      815
    • Publisher
      医歯薬出版
    • Related Report
      2011 Final Research Report
  • [Book] 口腔外科学2010

    • Author(s)
      白砂兼光, 古郷幹彦, 他
    • Total Pages
      815
    • Publisher
      医歯薬出版
    • Related Report
      2009 Annual Research Report
  • [Patent(Industrial Property Rights)] ES細胞培養用基礎培地2011

    • Inventor(s)
      岡本哲治、古江美保、浅島誠
    • Filing Date
      2011-01-07
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] アシルスペルミジン誘導体及び抗腫瘍用剤2011

    • Inventor(s)
      岡本哲治、小鹿一
    • Industrial Property Number
      2006-168926
    • Filing Date
      2011-02-25
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] Basal Medium for ES culturing2011

    • Inventor(s)
      Okamoto, Tetsuji, Furue Miho
    • Filing Date
      2011-04-12
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] BASAL MEDIUM FOR ES CELLCULTURING2010

    • Acquisition Date
      2010-04-28
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] :Defined Medium for Human ESCell Culture

    • Inventor(s)
      岡本哲治、楠田美保、デンリー・サトー、ピーター・アンドリュース
    • Industrial Property Number
      2009-518958
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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