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Isolation and identification of oral cancer stem cells and development of specific therapy for cancer stem cells

Research Project

Project/Area Number 21390544
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHyogo Medical University

Principal Investigator

URADE Masahiro  兵庫医科大学, 医学部, 教授 (70104883)

Co-Investigator(Kenkyū-buntansha) NOGUCHI Kazuma  兵庫医科大学, 医学部, 講師 (50309473)
HASHITANI Susumu  兵庫医科大学, 医学部, 助教 (00330449)
KISHIMOTO Hiromitsu  兵庫医科大学, 医学部, 准教授 (30291818)
Research Collaborator SEGAWA Emi  兵庫医科大学, 医学部, 助教 (00553778)
ZUSHI Yuusuke  兵庫医科大学, 医学部, 助教 (80581206)
HIROMOTO Takashi  兵庫医科大学, 医学部, 大学院生
YAMAMURA Michiyo  兵庫医科大学, 医学部, 大学院生
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords臨床腫瘍学 / 口腔癌 / 癌幹細胞 / 細胞培養 / 造腫瘍性 / 抗癌剤耐性 / 上皮・間葉移行 / 免疫組織化学 / 浩腫瘍性
Research Abstract

The aim of this study is to isolate and identify oral cancer stem cells(CSC) that are considered to be involved in tumor recurrence and metastasis, and to develop the therapy specific for CSC. Various cultured oral squamous carcinoma cells contained SP cells that are included in side population and considered CSC, in 0.04.0.4% of total cell population. The ratio included had a tendency to be increased in cancer cells overexpressing cyclooxygenase-2, cisplatin-resistant cancer cells expressing multidrug resistance gene MDR-1, and cancer cells showing poor differentiation and high invasiveness. There is a possibility to find out a clue for specific therapy by using these CSC-like cells.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (26 results)

  • [Journal Article] An in vitro multistep carcinogenesis model for both HPV-positive and-negative human oral squamous cell carcinomas2011

    • Author(s)
      Yusuke Zushi, Mako Narisawa-Saito, Kazuma Noguchi, Yuki Yoshimatsu, Takashi Yugawa, Nagayasu Egawa, Masatoshi Fujita, Masahiro Urade, Tohru Kiyono
    • Journal Title

      Am J Cancer Res

      Volume: 1(7) Pages: 869-881

    • NAID

      130004707939

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma : Relation to cell differentiation2011

    • Author(s)
      Takashi Hiromoto, Kazuma Noguchi, Michiyo Yamamura, Yusuke Zushi, Emi Segawa, Kazuki Takaoka, Kuniyasu Moridera, Hiromitsu Kishimoto, Masahiro Urade
    • Journal Title

      Oncol Rep

      Volume: 26 Pages: 1415-1421

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma : Relations to cell differentiation2011

    • Author(s)
      Hiromoto T, et al
    • Journal Title

      ONCOLOGY REPORTS

      Volume: 26 Pages: 1415-1421

    • DOI

      10.3892/or.2011.1429

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinomas2011

    • Author(s)
      Zushi Y, et al
    • Journal Title

      American Journal of Cancer Research

      Volume: 1 Pages: 869-881

    • NAID

      130004707939

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Promotion of hematogeneous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-22010

    • Author(s)
      Emi Segawa, Hiromitsu Kishimoto, Kazuki Takaoka, Kazuma Noguchi, Susumu Hashitani, Kazunari Sakurai, Masahiro Urade
    • Journal Title

      Oncol Rep

      Volume: 24 Issue: 3 Pages: 733-739

    • DOI

      10.3892/or_00000915

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antitumor effects of inhibitors of nitric oxide synthase or cyclooxygenase-2 on human KB carcinoma cells overexpressing COX-22010

    • Author(s)
      Nao Ohtsu, Kazuki Takaoka, Emi Segawa, Susumu Hashitani, Kazuma Noguchi, HiromitsuKishimoto, Masahiro Urade
    • Journal Title

      Oncol Rep

      Volume: 24 Issue: 1 Pages: 31-36

    • DOI

      10.3892/or_00000825

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antitumor effects of inhibitors of nitric oxide synthase or cyclooxygenase-2 on human KB carcinoma cells overexpressing COX-22010

    • Author(s)
      Ohtsu N, et al.
    • Journal Title

      Oncology Reports

      Volume: 24 Pages: 31-36

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Promotion of hematogenous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-22010

    • Author(s)
      Segawa E, et al.
    • Journal Title

      Oncology Reports

      Volume: 24 Pages: 733-739

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Promotion of hematogeneous metastatic potentials in human KB carcinoma cells with overexpression of cyclooxygenase-22010

    • Author(s)
      Emi Segawa, et al.
    • Journal Title

      Oncology Reports 22(In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 口腔扁平上皮癌培養細胞を用いたシスプラチン耐性における銅排出輸送体ATP7Bの発現とその関与の検討2011

    • Author(s)
      吉川恭平、野口一馬、山村倫世、頭司雄介、高岡一樹、岸本裕充、浦出雅裕
    • Organizer
      第48回日本口腔組織培養学会学術大会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 不死化ヒト正常ケラチノサイト(HTK)を用いた口腔がんのHPV陽性、陰性モデル解析2011

    • Author(s)
      頭司雄介、野口一馬、山村倫世、高岡一樹、瀬川英美、岸本裕充、浦出雅裕
    • Organizer
      第48回日本口腔組織培養学会学術大会
    • Place of Presentation
      千葉
    • Year and Date
      2011-11-19
    • Related Report
      2011 Final Research Report
  • [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔がんのHPV陽性、陰性モデルの解析2011

    • Author(s)
      頭司雄介, 他
    • Organizer
      第48回日本口腔組織培養学会学術大会
    • Place of Presentation
      明海大学浦安キャンパス
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] 口腔扁平上皮癌培養細胞を用いたシスプラチン耐性における銅排出輸送体ATP7Bの発現とその関与の検討2011

    • Author(s)
      吉川恭平, 他
    • Organizer
      第48回日本口腔組織培養学会学術大会
    • Place of Presentation
      明海大学浦安キャンパス
    • Year and Date
      2011-11-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of NOS inhibitors and CXCR4 antagonist on tumor growth and metastasis of human adenoid cystic carcinoma xenografted into nude mice2011

    • Author(s)
      Kazuki Takaoka, Sayaka Hidaka, Sususmu Hashitani, Yusuke Zushi, Michiyo Yamamura, Emi Segawa, Kazuma Noguchi, Hiromitsu Kishimoto, Masahiro Urade
    • Organizer
      20^<th> International Conference on Oral and Maxillofacial Surgery
    • Place of Presentation
      Santiago, Chile
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] An in vitro multistep carcinogenesis model for both HPV-positive and-negative human oral squamous cell carcinoma2011

    • Author(s)
      YusukeZushi, Kazuma Noguchi, Michiyo Yamamura, Kazuki Takaoka, Kuniyasu Moridera, Emi Segawa, Shin Okui, Hiromitsu Kishimoto, Masahiro Urade
    • Organizer
      20^<th> International Conference on Oral and Maxillofacial Surgery
    • Place of Presentation
      Santiago, Chile
    • Year and Date
      2011-11-02
    • Related Report
      2011 Final Research Report
  • [Presentation] An in vitro multistep carcinogenesis model for both HPV-positive and -negative human oral squamous cell carcinoma2011

    • Author(s)
      Zushi Y, et al
    • Organizer
      20^<th> International Conference on Oral and Maxillo facial Surgery
    • Place of Presentation
      サンチャゴ、チリ
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of NOS inhibitors and CXCR4 antagonist on tumor growth and metastasis of human adenoid cystic carcinoma xenografted into nude mice2011

    • Author(s)
      Takaoka K, et al
    • Organizer
      20^<th> International Conference on Oral and Maxillo facial Surgery
    • Place of Presentation
      サンチャゴ、チリ
    • Year and Date
      2011-11-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔癌のHPV陽性、陰性モデルの解析2011

    • Author(s)
      頭司雄介、野口一馬、山村倫世、高岡一樹、森寺邦康、瀬川英美、奥井森、岸本裕充、浦出雅裕
    • Organizer
      第56回(社)日本口腔外科学会学術大会
    • Place of Presentation
      大阪
    • Year and Date
      2011-10-21
    • Related Report
      2011 Final Research Report
  • [Presentation] 不死化ヒト正常舌ケラチノサイト(HTK)を用いた口腔癌のHPV陽性、陰性モデルの解析2011

    • Author(s)
      頭司雄介, 他
    • Organizer
      第56回(社)日本口腔外科学会総会・学術大会
    • Place of Presentation
      大阪国際会議場、大阪
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 口腔扁平上皮癌細胞の上皮・間葉移行(EMT)におけるzyxinの関与2011

    • Author(s)
      瀬川英美、野口一馬、高岡一樹、頭司雄介、岸本裕充、浦出雅裕
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-05
    • Related Report
      2011 Final Research Report
  • [Presentation] 口腔扁平上皮癌細胞の上皮・間葉移行(EMT)におけるzyxinの関与2011

    • Author(s)
      瀬川英美, 他
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋国際会議場、名古屋
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] A functional analysis of zyxin in epithelial-mesenchymal transition of oral squamous cell carcinoma2011

    • Author(s)
      Michiyo Yamamura, Kazuma, Noguchi, Emi Segawa, Yusuke Zushi, Kazuki Takaoka, Hiromitsu Kishimoto, Masahiro Urade
    • Organizer
      16^<th> European Cancer Congress
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2011-09-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma : Relation to cell differentiation2011

    • Author(s)
      Kazuma Noguchi, Takashi Hiromoto, Michiyo Yamamura, Yusuke Zushi, Emi Segawa, Kazuki Takaoka, Kuniyasu Moridera, Hiromitsu Kishimoto, Masahiro Urade
    • Organizer
      16^<th> European Cancer Congress
    • Place of Presentation
      Stockholm, Sweden
    • Year and Date
      2011-09-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Up-regulation of neutrophil gelatinase-associated lipocalin in oral squamous cell carcinoma : Relation to cell differentiation2011

    • Author(s)
      Noguchi K, et al
    • Organizer
      16^<th> European Cancer Congress
    • Place of Presentation
      ストックホルム、スエーデン
    • Year and Date
      2011-09-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] A functional analysis of zyxin in epithelial-mesenchymal transition of oralsquamous cell carcinoma2011

    • Author(s)
      Yamamura M, et al
    • Organizer
      16^<th> European Cancer Congress
    • Place of Presentation
      ストックホルム、スエーデン
    • Year and Date
      2011-09-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] 口腔癌細胞におけるZyxinの発現および機能の検討2010

    • Author(s)
      山村倫世、野口一馬、瀬川英美、高岡一樹、頭司雄介、廣本孝史、岸本裕充、浦出雅裕
    • Organizer
      第47回日本口腔組織培養学会学術大会
    • Place of Presentation
      高知
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 口腔扁平上皮癌におけるNGALの発現2010

    • Author(s)
      廣本孝史、野口一馬、山村倫世、瀬川英美、頭司雄介、岸本裕充、浦出雅裕
    • Organizer
      第47回日本口腔組織培養学会学術大会
    • Place of Presentation
      高知
    • Year and Date
      2010-11-13
    • Related Report
      2011 Final Research Report
  • [Presentation] 口腔扁平上皮癌におけるNGALの発現2010

    • Author(s)
      廣本孝史, 他
    • Organizer
      第47回日本口腔組織培養学会学術大会
    • Place of Presentation
      高知城ホール(高知)
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 口腔癌細胞におけるZyxinの発現および機能の検討2010

    • Author(s)
      山村倫世, 他
    • Organizer
      第47回日本口腔組織培養学会学術大会
    • Place of Presentation
      高知城ホール(高知)
    • Year and Date
      2010-11-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] 口腔癌細胞におけるEMT markerとしてのzyxinの発現2010

    • Author(s)
      瀬川英美、野口一馬、山村倫世、高岡一樹、頭司雄介、岸本裕充、橋谷進、浦出雅裕
    • Organizer
      第55回(社)日本口腔外科学会学術大会
    • Place of Presentation
      千葉
    • Year and Date
      2010-10-16
    • Related Report
      2011 Final Research Report
  • [Presentation] 口腔癌細胞におけるEMT markerとしてのzyxinの発現2010

    • Author(s)
      瀬川英美, 他
    • Organizer
      第55回(社)日本口腔外科学会総会・学術大会
    • Place of Presentation
      幕張メッセ(千葉)
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 舌扁平上皮癌の形成・分化度におけるNeutrophil gelatinase-associated Lipocalin(NGAL)の発現2010

    • Author(s)
      廣本孝史、野口一馬、瀬川英美、頭司雄介、山村倫世、岸本裕充、浦出雅裕
    • Organizer
      第64回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-25
    • Related Report
      2011 Final Research Report
  • [Presentation] 舌扁平上皮癌の形成・分化度におけるNeutrophil gelatinase-associated Lipocalin (NGAL)の発現2010

    • Author(s)
      廣本孝史, 他
    • Organizer
      第64回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      札幌プリンスホテル(札幌)
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] 高転移能を有するマウス未分化癌に対するCXCR4拮抗剤とNOS阻害剤の腫瘍増殖および手に抑制効果2010

    • Author(s)
      日高沙弥香、橋谷進、頭司雄介、田中徳昭、高岡一樹、浦出雅裕
    • Organizer
      第64回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 高転移能を有するマウス未分化癌に対するCXCR4拮抗剤とNOS阻害剤の腫瘍増殖および転移抑制効果2010

    • Author(s)
      日高沙弥香, 他
    • Organizer
      第64回NPO法人日本口腔科学会学術集会
    • Place of Presentation
      札幌プリンスホテル(札幌)
    • Year and Date
      2010-06-24
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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