Real-time analysis of signal transduction pathways in chondrocytes under the mechanical stress

• Project/Area Number: 21390545
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: TAKAHASHI Ichiro 九州大学, 歯学研究院, 教授 (70241643)
• Co-Investigator(Kenkyū-buntansha): YAMAMOTO Teruko 東北大学, 大学院・歯学研究科, 教授 (00127250) ; IOI Hideki 九州大学, 大学病院, 講師 (10274474) ; TERAO Humie 九州大学, 大学院・歯学研究院, 助教 (10510018)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥14,300,000 (Direct Cost: ¥11,000,000、Indirect Cost: ¥3,300,000)
• Keywords: 歯科矯正学 / メカニカルストレス / リアルタイム解析 / 軟骨細胞 / 細胞内情報伝達 / リアルタイム観察 / 歯学矯正学

Research Abstract

We established ATDC5 cells expressing ERK-GFP fusion protein to investigate ERK activation and nuclear translocation in real time in a single cell. After application of mechanical stress by using micromanipulator, we observed a translocation of the ERK-GFP fusion protein from the cytoplasm to the nucleus immediately.

Research Products

• [Journal Article] Effects of retraction of anterior teeth and initial softtissue variables on lip changes in Japanese adults (2012)
  • Author(s): Hayashida H., et al
  • Journal Title: Eur J Orthod Volume: 33(4) Issue: 4 Pages: 419-426
  • DOI: 10.1093/ejo/cjq095
  • Peer Reviewed
• [Journal Article] Three-dimentional analysis of the pharyngeal airway morphology in growing Japanese females with and without cleft lip and palate (2012)
  • Author(s): Yoshihara M., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: (in press)
  • Peer Reviewed
• [Journal Article] Fibroblast growth factor 10 regulates Meckel' s cartilage formation during early mandibular morphogenesis in rats (2011)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Volume: 350(2)
  • Pages: 337-47
  • Peer Reviewed
• [Journal Article] In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar (2011)
  • Author(s): Honda J, Kobayashi I, Kiyoshima T, Nagata K, Wada H, Ookuma Y, Fujiwara H, Shiotsuka M, Takahashi I, Sakai H
  • Journal Title: J Mol Histol Volume: 42(1) Pages: 83-90
  • Peer Reviewed
• [Journal Article] Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics (2011)
  • Author(s): Deguchi T, Kurosaki H, Oikawa H, Kuroda S, Takahashi I, Yamashiro T, Takano-Yamamoto T
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 139(4)
  • Peer Reviewed
• [Journal Article] Evaluation of well-balanced lip position by Japanese orthodontic patients (2011)
  • Author(s): Shimomura T, Ioi H, Nakata S, Counts AL
  • Journal Title: Am J Orthod Dentofacial Orthop Volume: 139(4)
  • Peer Reviewed
• [Journal Article] The primary site of the acrocephalic feature in Apert syndrome is a dwarf図3:機械的刺激負荷によるERK-GFPの局在の経時的変化。機械的刺激負荷により、ERK-GFPの核移行が顕著に認められた。0 9. 9 19. 8 29. 7 39. 6(秒)図4:核の蛍光強度(核・細胞質のOD比)解析。機械的刺激負荷により、核における蛍光強度が増加した。図5:軟骨分化過程における機械的刺激の果たす役割cranial base with ac (2011)
  • Author(s): Nagata M, GH. Nuckolls, X Wang, L Shum, Seki Y, Kawase T, Takahashi K, Nonaka K, Takahashi I, AA. Nomar, Suzuki K, Slavkin HC
  • Journal Title: Bone Volume: 48 Pages: 847-856
  • Peer Reviewed
• [Journal Article] Effects of retraction of anterior teeth and initial soft tissue variables on lip changes in Japanese adults. (2011)
  • Author(s): Hayashida H, Ioi H, Nakata S, Takahashi I, Counts AL
  • Journal Title: Eur J Orthod Volume: 33(4) Pages: 419-26
  • Peer Reviewed
• [Journal Article] phospholipase C-related, but catalytically inactive protein, in bone formation (2011)
  • Author(s): Tsutsumi K, Matsuda M, Kotani M, Mizokami A, Murakami A, Takahashi I, Terada Y, Kanematsu T, Fukami K, Takenawa T, Jimi E, Hirata M. Involvement of PRIP
  • Journal Title: J Biol Chem Volume: 286(35) Pages: 31032-42
  • Peer Reviewed
• [Journal Article] Three-dimensional analyisis of the temporomandibular joint and fossa-condyle relationship (2011)
  • Author(s): Endo M, Terajima M, Goto T, Tokumori K, Takahashi I
  • Journal Title: Orthodontics Volume: 12(3) Pages: 210-221
  • Peer Reviewed
• [Journal Article] Comparison of orthodontic treatment outcomes in adults with skeletal open bite between conventional edgewise treatment and implant-anchored orthodontics (2011)
  • Author(s): Deguchi T., et al
  • Journal Title: Am J Orthod Dentofacial Orthopedics Volume: 139(4) Issue: 4 Pages: 60-68
  • DOI: 10.1016/j.ajodo.2009.04.029
  • Peer Reviewed
• [Journal Article] The primary site of the acrocephalic feature in Apert syndrome is a dwarf cranical base with accelerated chondrocytic differentiation due to aberrant activation of the FGFR2 signaling (2011)
  • Author(s): Nagata M., et al
  • Journal Title: Bone Volume: 48 Issue: 4 Pages: 847-856
  • DOI: 10.1016/j.bone.2010.11.014
  • Peer Reviewed
• [Journal Article] Three-dimensional analyisis of the temporpmandibular joint and fossa-condyle relationaship (2011)
  • Author(s): Endo M., et al
  • Journal Title: Orthodontics Volume: 12(3) Pages: 2-13
  • Peer Reviewed
• [Journal Article] Involvement of PRIP, Phospholipase C-related, but Catalytically Inactive Protein, in Bone Formation (2011)
  • Author(s): Koshiro Tsutsumi
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 35 Pages: 31032-31042
  • DOI: 10.1074/jbc.m111.235903
  • Peer Reviewed
• [Journal Article] Fibroblast Growth Factor 10 Regulates Meckel's Cartilage Formation during Early Mandibular Morphogenesis in Rats. (2011)
  • Author(s): Terao F, Takahashi I, 他
  • Journal Title: Developmental Biology Volume: 350 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Expression of collagen type 1 and 2 on articular cartilage in a rat knee contracture model (2010)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, TakahashiI, Sasano Y, Onoda Y, Suda H, Itoi E
  • Journal Title: Connective Tissue Research Volume: 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament (2010)
  • Author(s): Shimonishi M, Takahashi I, Terao F, Komatsu M, Kikuchi M
  • Journal Title: J Periodont Res Volume: 45 Pages: 309-316
  • Peer Reviewed
• [Journal Article] Expression of collagen type 1 and 2 on articular cartilage in a rat knee contracture model. (2010)
  • Author(s): Hagiwara H, Takahashi I, 他
  • Journal Title: Connective Tissue Research Volume: 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Expression of collagen types I and II on articular cartilage in a rat knee contracture model (2010)
  • Author(s): Hagiwara Y, Ando A, Chimoto E, Tsuchiya M, Takahashi I, et al
  • Journal Title: Connect Tissue 51 Pages: 22-30
  • Peer Reviewed
• [Journal Article] Induction of MMP-2 at the interface between epithelial cells and fibroblasts from human periodontal ligament. (2010)
  • Author(s): Shimonishi M, Takahashi I, Terao F, Komatsu M, Kikuchi M
  • Journal Title: J Periodont Res (in press)
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of the response to mechanical stimulation during chondrocyte differentiation (2010)
  • Author(s): Takahashi I, Masuda T, et al
  • Journal Title: Interface Oral Health Science (in press)
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of mechanical stress response during chondrogenesis, J Biomechanical Science and Engineering (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Ando T, Sasano Y, Yamamoto T, Suzuki O
  • Volume: 4(3)
  • Pages: 307-317
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of mechanical stress response during chondrogenesis (2009)
  • Author(s): Takahashi I, Masuda T, et al
  • Journal Title: J Biomech Sci Eng 4 Pages: 307-317
  • NAID: 130000129002
  • Peer Reviewed
• [Presentation] (2011)
  • Author(s): Nomura S, Tsuru K, Valanezhad A, Matsuya S, Takahashi I, Ishikawa K. Fabrication of Carbonate Apatite Block from Calcium Sulfate by Hydrothermal Treatment.
  • Organizer: BIOCERAMICS
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-07-11
• [Presentation] Fabrication of Carbonate Apatite Block from Calcium Sulfate by Hydrothermal Treatment (2011)
  • Author(s): Nomura S
  • Organizer: BIOCERAMICS 23
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2011-07-11
• [Presentation] Effects of buccal corridors on smile esthetics in Korean and Japanese orthodontic patients (2011)
  • Author(s): Ioi H, Kang S, Shimomura T, Kim S, Park S, Son W, Takahashi I, Counts AL
  • Organizer: American Association of Orthodontists
  • Place of Presentation: Chicago, IL
  • Year and Date: 2011-05-14
• [Presentation] Effects of buccal corridors on smile esthetics in Korean and Japanese orthodontic patients (2011)
  • Author(s): Ioi H
  • Organizer: American Association of Orthodontists
  • Place of Presentation: Chicago, USA
  • Year and Date: 2011-05-14
• [Presentation] Expression pattern and possible function of 0thymosin beta-10 in developing tooth gern compared with thymosin bata-4 (2011)
  • Author(s): Shiotsuka M, Wada H, Kiyoshima T, Kobayashi I, Nagata K, Fujiwara H, Takahashi I, Sakai H
  • Organizer: The 6th International Joint Symposium on"Dental and Craniofacial Morphogenesis and Tissue Regeneration"and"Oral Health Science"(第6回国際シンポジウム)
  • Place of Presentation: Fukuoka
• [Presentation] Effects of allergic inflammation on orthodontically induced tooth root resorption (2011)
  • Author(s): Murata N, Ioi H, Ohuchi M, Takao T, Oida H, Yamaza T, Takahashi I, Kido MA.
  • Organizer: The 6th International Joint Symposium on"Dental and Craniofacial Morphogenesis and Tissue Regeneration"and"Oral Health Science"(第6回国際シンポジウム)
  • Place of Presentation: Fukuoka
• [Presentation] 胎生期下顎形成期におけるFGF10によるメッケル軟骨形態の制御 (2011)
  • Author(s): 寺尾文恵、高橋一郎、三谷英稔、春山直人、笹野泰之、鈴木治、山本照子
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪市
• [Presentation] FGF10 contributes Meckel. s cartilage formation in early mandibular development (2011)
  • Author(s): Terao F
  • Organizer: The 6th International Workshop on Nano-, Bio-and Amorphous Materials
  • Place of Presentation: Zao
• [Presentation] FGF10 contributes Meckel's cartilage formation in early mandibular development (2011)
  • Author(s): Terao F
  • Organizer: he 6th International Workshop on Nano-, Bio- and Amorphous Materials
  • Place of Presentation: Zao, Miyagi
• [Presentation] Mechano-Biology of cytoskeletal reorganization in differentiating chondrocytes. (2010)
  • Author(s): Takahashi I
  • Organizer: International Workshop on Nano, Bio and Amorphous Materials
  • Place of Presentation: 宮城、蔵王
  • Year and Date: 2010-08-09
• [Presentation] Fibroblast growth factor 10 and Meckel. s cartilage formation in the early mandibular morphogenesis (2010)
  • Author(s): Terao F, Takahashi I, Mitani H, Haruyama N, Sasano Y, Suzuki O, Takano-Yamamoto T
  • Organizer: The 5th International Symposium on Dental and Craniofacial Morphogenesis and Tissue Regeneration : A View from Stem Cell Research(第5回国際シンポジウム「口腔組織の再生・再建医療研究」
  • Place of Presentation: 福岡市
  • Year and Date: 2010-02-05
• [Presentation] Studies on characterization of the gene structure and transcriptional regulation of a novel inositol 1, 4, 5-trisphosphate binding protein, possibly involved in bone metabolism (2010)
  • Author(s): Ayako Murakami, Miho Matsuda, Koushirou Tsutsumi, Masato Hirata, and Ichiro Takahashi
  • Organizer: The 5th International Symposium on Dental and Craniofacial Morphogenesis and Tissue Regeneration : A View from Stem Cell Research(第5回国際シンポジウム「口腔組織の再生・再建医療研究」
  • Place of Presentation: 福岡市
  • Year and Date: 2010-02-05
• [Presentation] of cytoskeletal reorganization in differentiating chondrocytes. International Workshop on Nano (2010)
  • Author(s): Takahashi I : Mechano-Biology
  • Organizer: Bio and Amorphous Materials
  • Place of Presentation: Miyagi-Zao
• [Presentation] 歯胚におけるphosphoglycerate kinase(Pgk) 1タンパクの局在とその新しい役割について (2010)
  • Author(s): 本田淳也,小林家吉,坂井英隆,高橋一郎
  • Organizer: 第69回日本矯正歯科学会大会
  • Place of Presentation: 横浜市
• [Presentation] Lip-morphological differences between classIIand classIIImolocclusion before/after orthodontic treatment. (2010)
  • Author(s): Islam R, Kitahara T, Naher L, Takahashi I
  • Organizer: International Association for Dental Research
  • Place of Presentation: Spain-Barcelona
• [Presentation] Effect of allergic inflammation on the orthodontically induced root resorption. (2010)
  • Author(s): Murata N, Ioi H, Takahashi I, Kido MA
  • Organizer: International Association for Dental Research
  • Place of Presentation: Spain-Barcelona
• [Presentation] Intracellular signal transduction during mechano-response in differentiating chondrocytes (2009)
  • Author(s): Takahashi I, Masuda T, Kohsaka K, Terao F, Anada T, Sasano Y, Takano-Yamamoto T, Suzuki O
  • Organizer: JSPS-KOSEF Asian Core Program, Nano/Amorphous Materials and Interface Science Symposium
  • Place of Presentation: Tohgatta, Miyagi
  • Year and Date: 2009-08-07
• [Presentation] Effects of buccal corridors on smile esthetics in Japanese (2009)
  • Author(s): Ioi H, Nakata S, Counts AL
  • Organizer: 109th annual session of American Association of Orthodontists
  • Place of Presentation: Boston, USA
• [Remarks]
  • URL: http://www.ortho.dent.kyushu-u.ac.jp/
• [Remarks]
  • URL: http://www.ortho.dent.kyushu-u.ac.jp/