Influence of ocean warming on fish reproduction: international joint survey in East Asian region

• Project/Area Number: 21405001
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Environmental dynamic analysis
• Research Institution: Nagasaki University
• Principal Investigator: SOYANO Kiyoshi 長崎大学, 大学院・水産・環境科学総合研究科, 教授 (80260735)
• Co-Investigator(Kenkyū-buntansha): NAGAE Masaki 長崎大学, 大学院・水産・環境科学総合研究科, 准教授 (00315227)
• Co-Investigator(Renkei-kenkyūsha): MAKAMURA Masaru 琉球大学, 熱帯生物圏研究センター, 教授 (10101734) ; AOKI Jyunya 長崎大学, プロジェクト研究員 (20432990)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 海洋温暖化 / 繁殖 / 配偶子形成 / ホルモン遺伝子 / 東シナ海 / ホルモン遺伝 / 魚類

Research Abstract

We planned to investigate effects of endocrine disrupting chemicals (EDCs) and grovel warming on fish reproduction and next generation production in East Asia. In this experiment including oversea research, we used endocrine, physiological, and histological methods for obtaining the information about EDCs and water temperature effects. The grey mullet, Japanese common goby, groupers, dojo loach were selected as a suitable wildlife for these experiments and survey. In this project, EDCs contamination was observed in coastal waters in East Asia, and influence of grovel warming on fish reproduction, such as a changes in spawning period, an induction of abnormality in early development, a reduction of ovulated eggs, were indicated. In addition, fish reproduction was inhibited by the combined effect of high temperature and EDCs.

Research Products

• [Journal Article] Comparative responsiveness to natural and synthetic estrogens of fish species commonly used in the laboratory and field monitoring (2012)
  • Author(s): Lange A, Katsu Y, Miyagawa S, Ogino Y, Urushitani H, Kobayashi T, Hirai T, Shears JA, Nagae M, Yamamoto J, Ohnishi Y, Oka T, Tatarazako N, Ohta Y, Tyler CR, Iguchi T
  • Journal Title: Aquatic Toxicology Volume: 109 Pages: 250-258
  • DOI: 10.1016/j.aquatox.2011.09.004
  • Peer Reviewed
• [Journal Article] Effects of ethynylestradiol on vitellogenin synthesis and sex differentiation in juvenile grey mullet (Mugil cephalus) persist after long-term exposure to a clean environment (2011)
  • Author(s): Aoki, J., Hatsuyama, A., Hiramatsu, N., Soyano, K.
  • Journal Title: Comp. Physiol. Biochem. Volume: 154C Pages: 346-352
• [Journal Article] Annual changes in ovarian development and sex steroid levels in cultured female dojo loach Misgurnus anguillicaudatus (2011)
  • Author(s): Solomon Kiros, Aoki, J., Soyano, K.
  • Journal Title: Aquacult. Sci. Volume: 59 Pages: 19-28
  • NAID: 10029847162
• [Journal Article] Evaluation of environmental estrogenic activity in the Yangtze River estuary: utilization of vitellogenin production in red lip mullet (2011)
  • Author(s): Wu, M. Q., Hong, L., Luo, W., Hiramatsu, N., Aoki, J., Soyano, K., Itashiki, Y., Zhong, J., Hara, A.
  • Journal Title: Aquacult. Sci. Volume: 59
  • NAID: 10029847348
• [Journal Article] Annual changes in testicular development and plasma sex steroids in the captive male dojo loach Misgurnus anguillicaudatus (2011)
  • Author(s): Solomon Kiros, Aoki, J., Park, C. B., Soyano, K.
  • Journal Title: Ichthyo.l Res. Volume: 58 Pages: 217-224
  • NAID: 10029296007
• [Journal Article] Annual changes in testicular development and plasma sex steroids in the captive male dojo loach Misgurnus anguillicaudatus (2011)
  • Author(s): Solomon Kiros, Aoki, J., Park, C.B., Soyano, K.
  • Journal Title: Ichthyological Research Volume: 55 Issue: 3 Pages: 619-625
  • DOI: 10.1007/s10228-011-0213-7
  • NAID: 10029296007
  • Peer Reviewed
• [Journal Article] Annual changes in ovarian development and sex steroid levels in cultured female dojo loach Misgurnus anguillicaudatus (2011)
  • Author(s): Solomon Kiros, Aoki, J., Soyano, K.
  • Journal Title: Aquaculture Science Volume: 59 Pages: 19-28
  • NAID: 10029847162
  • Peer Reviewed
• [Journal Article] Evaluation of environmental estrogenic activities in the Yangtze River estuary : Utilization of vitellogenin production in red lip mullet (2011)
  • Author(s): Wu M., Hong, L., Luo, W., Hiramatsu, N., Aoki, J., Soyano, K., Itashiki, Y., Zhong, J., Hara, A.
  • Journal Title: Aquaculture Science Volume: 59 Pages: 65-73
  • NAID: 10029847348
  • Peer Reviewed
• [Journal Article] Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus). (2010)
  • Author(s): Aoki, J., Nagae, M., Takao, Y., Hara, A., Lee, Y.D., Yeo, I.K., Lim, B.S., Park, C.B., Soyano, K.
  • Journal Title: Sci. Total Environ. Volume: 408 Pages: 660-665
• [Journal Article] The Effects of 17-estradiol on various reproductive parameters in the hermaphrodite fish, Kryptolebias marmoratus (2010)
  • Author(s): Park, C.B., Aoki, J., Lee, J.S., Nagae, M., Lee Y.D., Sakakura, Y., Hagiwara, A. Soyano, K.
  • Journal Title: Aquat. Toxicol. Volume: 96 Pages: 273-279
• [Journal Article] Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus) (2010)
  • Author(s): Aoki, J., Nagae, M., Takao, Y., Hara, A., Lee, YD., Yeo, IK., Lim, BS., Park, CB., Soyano, K.
  • Journal Title: Science of the Total Environment Volume: 408 Pages: 660-665
  • Peer Reviewed
• [Journal Article] The Effects of 17β-estradiol on various reproductive parameters in the hermaphrodite fish, Kryptolebias marmoratus. (2010)
  • Author(s): Park, C.B., Aoki, J., Lee, J.S., Nagae, M., Lee, Y.D., Sakakura, Y., Hagiwara, A., Soyano, K.
  • Journal Title: Aquatic Toxicology 96 Pages: 273-279
  • Peer Reviewed
• [Presentation] The evaluation of estrogenic activity in the tidal flat using VTG gene expression of the mudskipper (2011)
  • Author(s): Suzuki, A., Nagae, M., Soyano, K.
  • Organizer: The 8^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
  • Year and Date: 2011-10-28
• [Presentation] Seasonal changes in oocyte development of the blacktip grouper, Epinephelus fasciatus (2011)
  • Author(s): Katayama, S., Izumida, D., Lee, Y.D., Soyano, K.
  • Organizer: The 8^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
  • Year and Date: 2011-10-28
• [Presentation] The evaluation of estrogenic activity in the tidal flat using VTG gene expression of the mudskipper (2011)
  • Author(s): Suzuki, A., Nagae, M., Soyano, K.
  • Organizer: The 8^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: 長崎
  • Year and Date: 2011-10-28
• [Presentation] Reproductive disruption in aquatic wildlife exposed to EDCs: past, present, and future (2011)
  • Author(s): Soyano, K., Nagae, M.
  • Organizer: 13th Annual Scientific Workshop UK-Janapn Co-operation for Research on Endocrine Disrupters in the Aquatic Environment
  • Place of Presentation: Northumberland, Matfen Hall, UK
• [Presentation] トビハゼを用いた干潟における環境ホルモン汚染の影響評価 (2011)
  • Author(s): 鈴木絢子・長江真樹・征矢野清
  • Organizer: 第14回環境ホルモン学会研究発表会
  • Place of Presentation: 東京
• [Presentation] 環東シナ海地域における大気中多環芳香族炭化水素類の動態と年間変動 (2011)
  • Author(s): 山中潤二, 郭雪松, Yu Tao, 山城詩織, 久保隆, 長江真樹, 西山雅也, 岡田二郎, 河本和明, 高辻俊宏, 武政剛弘, Yeo In-Kyu, Lee Ki-Ho, Lee Young-Don, 高尾雄二
  • Organizer: 第20回環境化学討論会
  • Place of Presentation: 熊本
• [Presentation] International survey of the environmental pollution by endocrine disrupting chemicals in the East China Sea (2011)
  • Author(s): Soyano, K.
  • Organizer: 日本水産学会シンポジウム
  • Place of Presentation: 長崎
• [Presentation] Effects of long term exposure to constant temperature-photoperiod regimes on the reproductive activity in female dojo loach, Misgurnus anguillicaudatus (2011)
  • Author(s): Solomon Kiros, Aoki, J., Park, C.B., Soyano, K.
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
• [Presentation] ボラを調査対象生物とした東シナ海沿岸域にける環境ホルモン汚染の実態調査 (2011)
  • Author(s): 板敷祥昌・Wu Meiqin・Zhong Junsheng・青木純哉・平松尚志・原彰彦・征矢野清
  • Organizer: 平成22年度日本水産学会秋季大会
  • Place of Presentation: 京都
• [Presentation] Environmental contamination by estrogenic chemicals in Japanese and Chinese coastal waters (2010)
  • Author(s): Itashiki, Y., Aoki, J., Park, CB., Wu, M., Zhong, J., Soyano, K..
  • Organizer: The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: 大韓民国・剤州島
  • Year and Date: 2010-11-18
• [Presentation] Pheromone-induced final oocyte maturation and ovulation in honeycomb grouper, Epinephelus merra (2010)
  • Author(s): Izumida, D., Murata, R., Nakamura, M., Soyano, K..
  • Organizer: The 7th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: 大韓民国・剤州島
  • Year and Date: 2010-11-18
• [Presentation] Combined effects of endocrine disrupting chemicals and global warming in the aquatic environment of fish reproduction (2010)
  • Author(s): Soyano, K.
  • Organizer: The 12th UK-Japan Annual Scientific Workshop on Environmental Endocrine Disrupting Chemicals
  • Place of Presentation: 英国
  • Year and Date: 2010-11-03
• [Presentation] Effect of long term exposure to constant temperature-photoperiod regimes on the reproductive activity in female dojo loach, Misgurnus anguilli caudatus (2010)
  • Author(s): Solomon Kiros Aoki, J., Park, CB., Soyano, K..
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-24
• [Presentation] カンモンハタ雌の最終成熟および排卵における性フェロモンの存在とその役割 (2010)
  • Author(s): 泉田大介・村田良介・中村将・山田秀秋・町口裕二・征矢野清
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-24
• [Presentation] ボラを調査対象生物とした東シナ海沿岸域における環境ホルモン汚染の実態調査 (2010)
  • Author(s): 板敷祥昌・Wu, M., Zhong, J., Park, CB., 青木純哉・平松尚志・原彰彦・征矢野清
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 京都大学
  • Year and Date: 2010-09-24
• [Presentation] カンモンハタの成熟サイズにおける地域差. (2010)
  • Author(s): 泉田大介, 中村將, 征矢野清
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
  • Year and Date: 2010-03-27
• [Presentation] Combined effects of endocrine disrupting chemicals and global warming in aquatic environment on fish reproduction (2010)
  • Author(s): Soyano, K.
  • Organizer: 12th Annual Scientific Workshop UK-Janapn Co-operation for Research on Endocrine Disrupters in the Aquatic Environment
  • Place of Presentation: Northumberland, Matfen Hall, UK(招待講演)
• [Presentation] Environmental contamination by estrogenic chemicals in Japanese and Chinese coastal waters (2010)
  • Author(s): Itashiki, Y., Aoki, J., Park, C.B., Wu, M., Zhong, J., Soyano, K
  • Organizer: The 7^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Jeju, Korea
• [Presentation] Effect of temperature on growth and gonadal development in the mangrove killifish (2010)
  • Author(s): Ura, T., Park, C.B., Soyano, K.
  • Organizer: The 7^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Jeju, Korea
• [Presentation] Analysis of estrogenic activity of water and sediment samples collected in estuaries around Northern part of Kyushu (2010)
  • Author(s): Seto, S., Koyanagi, Y., Hata, T., Araki, I., Nasu, K., Takao, Y., Nagae, M.
  • Organizer: The 7^<th> International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Jeju, Korea
• [Presentation] ホシガレイの性分化に与える水温の影響 (2010)
  • Author(s): 築山陽介, 征矢野清
  • Organizer: 平成22年度日本水産学会春季大会
  • Place of Presentation: 藤沢
• [Presentation] トビハゼの生殖腺発達に与える日長及び温度の影響. (2009)
  • Author(s): 祖父江勝, 長江真樹, 青木純哉, 征矢野清
  • Organizer: 2009年度日本水産学会秋期大会
  • Place of Presentation: 盛岡
  • Year and Date: 2009-10-01
• [Presentation] Effect of environmental factors on gonadal development of the mudskipper, Periophthalmus modestus. (2009)
  • Author(s): M.Sobue, J.Aoki, K.Soyano
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea.
  • Place of Presentation: 長崎大学
  • Year and Date: 2009-09-25
• [Presentation] Survey of estrogenic contamination in coastal areas f East Asia using Mugilidae (2009)
  • Author(s): Aoki, J., Lee, Y.D., Zhong, J., Soyano, K.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Estrogenic activity of coastal aquatic environments in China: evaluation using a model species, the red lip mullet (Chelon haematocheilus) (2009)
  • Author(s): Wu, M., Aoki, J., Hiramatsu, N., Hara, A., Soyano, K., Zhong, J.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Estrogenic activity in coastal areas around Kyusyu by measuring male serum vitellogenin in Japanese common goby (2009)
  • Author(s): Nagae, M., Hata, T., Takemura, Y., Shiroyama, K., Nasu, K., Takao, Y., Ohkubo, N., Matsubara, T., Soyano, K.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Effects of soybean-derived phytoestrogens on vitellogenin gene expression in the grey mullet, Mugil cephalus (2009)
  • Author(s): Itashiki, Y., Aoki, J., Park, C.B., Soyano, K.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Changes in gene expression of specific biomarker (spiggin) and androgen receptors in kidney of stickleback treated with exogenous androgen (2009)
  • Author(s): Matsuo, S., Ohkubo, N., Matsubara, T., Soyano, K., Katsiadaki, I., Scott, A.P., Nagae, M.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Effect of environmental factors on gonadal development of the mudskipper, Periophthalmus modestus (2009)
  • Author(s): Sobue, M., Aoki, J., Soyano, K.
  • Organizer: The 6th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Nagasaki
• [Presentation] Effects of temperature and photoperiod on ovarian development in female dojo loach, Misgurnus anguillicaudatus (2009)
  • Author(s): Solomon Kiros, Aoki, J., Park, C.B., Soyano, K.
  • Organizer: 2009年度日本水産学会秋期大会
  • Place of Presentation: 盛岡
• [Presentation] トビハゼの生殖腺発達に与える日長及び温度の影響 (2009)
  • Author(s): 祖父江勝, 長江真樹, 青木純哉, 征矢野清
  • Organizer: 2009年度日本水産学会秋期大会
  • Place of Presentation: 盛岡
• [Presentation] マハゼビテロゲニンを指標とした九州北西部河口域のおける環境エストロゲン汚染評価 (2009)
  • Author(s): 長江真樹, 秦拓朗, 武村幸紀, 城山健一郎, 奈須一晃, 高尾雄二, 大久保伸幸, 松原孝博, 征矢野清
  • Organizer: 第12回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] ボラ科魚類を用いた東アジア沿岸域における環境ホルモン汚染の実態調査 (2009)
  • Author(s): 青木純哉, 板敷祥昌, Meiqin Wu, Junsheng Zhong, Young-Don Lee, 征矢野清
  • Organizer: 第12回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] 自家受精魚マングローブキリフィッシュの生殖関連遺伝子の発現に及ぼすエストロジェンの影響 (2009)
  • Author(s): 朴昶範, 征矢野清, 李榮敦
  • Organizer: 第12回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] ボラのビテロジェニン遺伝子発現に及ぼす植物エストロジェンの影響 (2009)
  • Author(s): 板敷祥昌, 青木純哉, Chang-Beom Park, 征矢野清
  • Organizer: 第12回環境ホルモン学会
  • Place of Presentation: 東京