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Survey on members of sucrose-world in Asian tropical reef, namely in Thailand

Research Project

Project/Area Number 21405020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied biochemistry
Research InstitutionHokkaido University

Principal Investigator

KIMURA Atsuo  北海道大学, 大学院・農学研究院, 教授 (90186312)

Co-Investigator(Kenkyū-buntansha) MORI Haruhide  北海道大学, 大学院・農学研究院, 准教授 (80241363)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Keywordsショ糖 / 分泌 / 生物間相互作用
Research Abstract

Plants synthesize the starch in the leaves by photosynthesis. Leaf-starch is converted to sucrose, and transported to storage organ (e.g., tubers and fruit-bodies), followed by re-formation of starch (storage-starch). From many sites of bodies, plants secret sucrose, by which plants perform the cross-talk with environmental organisms (e.g., microorganisms and insects). Recently, it was found that huge amount of sucrose was secreted (this system is call as sucrose-world). The purpose of this research is survey of sucrose-world-members (environmental organisms and their enzymes) in Thailand, where high sunshine is available. As a result, the system of "sucrose -> polysaccharide -> secondary or ternary sugar" was investigated at sucrose secreted from roots. About flower bee, the enzyme converting sucrose to oligosaccharide was also investigated.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (119 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (29 results) (of which Peer Reviewed: 29 results) Presentation (87 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Expression of a secretory α-glucosidase II from Apis cerana indica in Pichia pastoris and its characterization2013

    • Author(s)
      Kaewmuangmoon J, Kilaso M, Leartsakulpa-nich U, Kimura K, Kimura A, Chanchao C
    • Journal Title

      BMC Biotechnol

      Volume: 13 Issue: 1 Pages: 16-16

    • DOI

      10.1186/1472-6750-13-16

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel mechanism for promotion of quercetin glycoside absorption by megalo α-1,6-glucosaccharide in the rat small intestine2013

    • Author(s)
      Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Okuyama M, Mori H, Kimura A, Ishizuka S, Hara H
    • Journal Title

      Food Chem

      Volume: 136(2) Issue: 2 Pages: 293-296

    • DOI

      10.1016/j.foodchem.2012.08.028

    • NAID

      120005228255

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amino Acids in Conserved Region II Are Crucial to Substrate Specificity, Reaction Velocity, and Regioselectivity in the Transglucosylation of Honeybee GH-13 α-Glucosidases2012

    • Author(s)
      Tagami T, Tanaka Y, Okuyama M, Mori H, Kimura A
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 10 Pages: 1967-1974

    • DOI

      10.1271/bbb.120473

    • NAID

      130004054024

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of some enzymatic properties of recombinant α-glucosidase III from the Thai honeybee, Apis cerana indica Fabricus2012

    • Author(s)
      Kaewmuangmoon J, Yoshiyama M, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C
    • Journal Title

      Afr J Biotechnol

      Volume: 11(96) Issue: 96 Pages: 16220-16232

    • DOI

      10.5897/ajb12.2402

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bacteroides thetaiotaomicron VPI- 5482 glycoside hydrolase family 66 homolog catalyzes dextranolytic and cyclization reactions2012

    • Author(s)
      Kim YM, Eiji Y, Kang MS, Nakai H, Saburi W, Okuyama M, Mori H, Funane K, Kim D, Kimura A
    • Journal Title

      FEBS J

      Volume: 279(17) Issue: 17 Pages: 3185-3191

    • DOI

      10.1111/j.1742-4658.2012.08698.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biochemical characterization of a cycloisomaltooligosaccharide glucanotransferase from Paenibacillus sp. 598K2012

    • Author(s)
      Suzuki R, Terasawa K, Kimura K, Fujimoto Z, Momma M, Kobayashi M, Kimura A, Funane K
    • Journal Title

      Biochim Biophys Acta

      Volume: 1824(7) Issue: 7 Pages: 919-924

    • DOI

      10.1016/j.bbapap.2012.04.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] α-Glucosidase-catalyzed novel reaction on 1,5-anhydrofructose, suggesting new metabolic pathway for production of glucose from starch2012

    • Author(s)
      Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M, Mori H, Kimura A
    • Journal Title

      J Biol Chem

      Volume: 287(27) Issue: 27 Pages: 22441-22444

    • DOI

      10.1074/jbc.c112.360909

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel dextranase catalyzing cycloisomaltooligosaccharide-formation and its identification of catalytic amino acids and their functions using chemical rescue approach.2012

    • Author(s)
      Kim YM
    • Journal Title

      J Biol Chem

      Volume: 287 Issue: 24 Pages: 19927-19935

    • DOI

      10.1074/jbc.m111.339036

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural elucidation of dextran degradation mechanism by Streptococcus mutans dextranase belonging to glycoside hydrolase family 662012

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Okuyama M, Mori H, Funane K, Kimura A
    • Journal Title

      J Biol Chem

      Volume: 287(24) Issue: 24 Pages: 19916-19926

    • DOI

      10.1074/jbc.m112.342444

    • NAID

      120005183151

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chemical constituents and free radical scavenging activity of corn pollen collected from Apis mellifera hives compared to floral corn pollen at Nan, Thailand2012

    • Author(s)
      Chantarudee A, Phuwapraisirisan P, Kimura K, Okuyama M, Mori H, Kimura A, Chanchao C
    • Journal Title

      BMC Complem Altern Med

      Volume: 12 Issue: 1 Pages: 45-45

    • DOI

      10.1186/1472-6882-12-45

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans2011

    • Author(s)
      Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H,Kimura A
    • Journal Title

      Acta Crystallogr F

      Volume: F67 Issue: 12 Pages: 1542-1544

    • DOI

      10.1107/s1744309111038425

    • NAID

      120003660602

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synthesis2011

    • Author(s)
      Kang HK, Kimura A, Kim D
    • Journal Title

      J Agric Food Chem

      Volume: 59(8) Issue: 8 Pages: 4148-4155

    • DOI

      10.1021/jf104629g

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Novel quantitative method for the degree of branching in dextran2011

    • Author(s)
      Kim YM, Kimura A, Kim D
    • Journal Title

      Food Sci Biotechnol

      Volume: 20(2) Issue: 2 Pages: 537-541

    • DOI

      10.1007/s10068-011-0075-9

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel quantitative method for the degree of branching in dextran2011

    • Author(s)
      Kim YM
    • Journal Title

      Food Sci Biotechnol

      Volume: 20 Pages: 537-541

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Truncation of N- and C-terminal regions of Streptococcus mutans dextranase enhances catalytic activity2011

    • Author(s)
      Kim YM
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 91 Issue: 2 Pages: 329-339

    • DOI

      10.1007/s00253-011-3201-y

    • Related Report
      2011 Annual Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular analysis of cycloisomaltooligosaccharide glucanotransferase and discovery of novel biosynthetic pathway of cycloisomaltooligosaccharide in Bacillus circulams T-30402011

    • Author(s)
      Funane K
    • Journal Title

      Bull Appl Glycosci

      Volume: 1 Pages: 179-185

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calcium Ion-Dependent Increase in Thermostability of Dextran Glucosidase from Streptococcus mutans2011

    • Author(s)
      Kobayashi M
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 8 Pages: 1557-1563

    • DOI

      10.1271/bbb.110256

    • NAID

      10029591699

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of C-terminal region of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulams T-30402011

    • Author(s)
      Funane K
    • Journal Title

      Biochim Biophys Acta

      Volume: VOL.1814 Pages: 428-434

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bioengineered glucansucrase of Leuconostoc mesenteroides for preferred oligo-saccharide synthesis using sucrose2011

    • Author(s)
      Kang HK
    • Journal Title

      J Agric Food Chem

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010

    • Author(s)
      Ryu HJ, Jin X, Lee JH, Woo HJ, Kim YM, Kim GJ, Seo ES, Kang HK, Kim J, Cho DL, Kimura A, Kim D
    • Journal Title

      Enzyme Microb Technol

      Volume: 47(5) Issue: 5 Pages: 212-215

    • DOI

      10.1016/j.enzmictec.2010.07.006

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rice Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell-wall-β-glucan-derived oligosaccharides and glycosides2010

    • Author(s)
      Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A, Ketudat-Cairns JR
    • Journal Title

      Plant Sci

      Volume: 179(3) Issue: 3 Pages: 273-280

    • DOI

      10.1016/j.plantsci.2010.05.013

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rice Os4BGlu12 is a wound-induced ^<【○!-R】>glucosidase that hydrolyzes cell-wall-^<【○!-R】>glucan-derived oligosaccharides and glycosides2010

    • Author(s)
      Opassiri R
    • Journal Title

      Plant Sci

      Volume: VOL.179 Pages: 273-280

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optimal expression and characterization of a fusion enzyme having dextransucrase and dextranase activities2010

    • Author(s)
      Ryu HJ
    • Journal Title

      Enzyme Microb Technol

      Volume: VOL.47 Pages: 212-215

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Suicide substrate-based inactivation of endodextranase by 1-epoxyalkyl <-D-glucopyranosides2010

    • Author(s)
      Kang HK
    • Journal Title

      J Appl Glycosci

      Volume: VOL.57 Pages: 111-117

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first α-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 312009

    • Author(s)
      Kang MS, Okuyama M, Mori H, Kimura A
    • Journal Title

      Biochimie

      Volume: 91(11-12) Issue: 11-12 Pages: 1434-1442

    • DOI

      10.1016/j.biochi.2009.07.018

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural comparison of Streptococcus mutans dextran glucosidase with glucoside hydrolases GH132009

    • Author(s)
      Hondoh H
    • Journal Title

      J Appl Glycosci VOL.56

      Pages: 111-117

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytic mechanism of retaining α-galactosidase belonging to glycoside hydrolase family 972009

    • Author(s)
      Okuyama M
    • Journal Title

      J Mol Biol VOL.392

      Pages: 1232-1241

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The first α-1,3-glucosidase from bacterial origin belonging to glycoside hydrolase family 312009

    • Author(s)
      Kang MS
    • Journal Title

      Biochimie VOL.91

      Pages: 1434-1442

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Catalytic reaction mechanism based on α-secondary deuterium isotope effects in hydrolysis of trehalose by European honeybee trehalase2009

    • Author(s)
      Mori H
    • Journal Title

      Biosci Biotechnol Biochem VOL.73

      Pages: 2466-2473

    • NAID

      10027547874

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 糖質酵素の基礎研究とその展開2012

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      静岡県立コンベンションアーツセンターグランシップ (静岡)
    • Year and Date
      2012-09-17
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanism of glycosylases catalyzing megalosaccharide production2012

    • Author(s)
      Mori H, Kim D, and Kimura A
    • Organizer
      BIT's 3rd Inaugural Symposium on Enzymes & Biocatalysis-2012, Xian Qujiang International Conference Center (Xian, China)
    • Year and Date
      2012-04-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 摂取するイソマルト糖の重合度はデキストラン硫酸ナトリウムによる大腸炎症状に影響する2012

    • Author(s)
      岩谷一史
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] 直鎖イソマルトメガロ糖はラット小腸におけるケルセチン配糖体吸収を促進する2012

    • Author(s)
      篠木亜季
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] Streptococcus mutans由来デキストラナーゼのX線結晶構造解析2012

    • Author(s)
      鈴木喜大
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼの活性向上と生成物サイズ特異性の制御2012

    • Author(s)
      鈴木龍一郎
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] デキストラナーゼに見出された『プロ酵素を介する活性化現象』の分子機構2012

    • Author(s)
      キムヨンミン
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional implication of amino acid residues in conserved region II of European honeybee α-glucosidase on substrate specificity, transglucosylation and substrate inhibition2012

    • Author(s)
      Lukana Ngiwsara
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacteroides thetaiotaomicron由来α-galactosidaseの求核残基変異酵素が触媒する糖転移反応の解析2012

    • Author(s)
      渡辺健一
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都女子大(京都市)
    • Year and Date
      2012-03-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enzymatic synthesis of acarviosyl- maltooligosaccharides and their inhibitory effects on α-glucosidase2012

    • Author(s)
      Tagami T, Tanaka Y, Mori H, Okuyama M, Kimura A
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Related Report
      2012 Final Research Report
  • [Presentation] Amino acid residues in conserved region II involved in specific function of European honeybee (Apis mellifera) α-glucosidase isozymes2012

    • Author(s)
      Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Related Report
      2012 Final Research Report
  • [Presentation] Enzymatic synthesis of acarviosyl-maltooligosaccharides and their inhibitory effects on α-glucosidase.2012

    • Author(s)
      Tagami T
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Amino acid residues in conserved region II involved in specific function of European honeybee (Apis mellifera) α-glucosidase isozymes.2012

    • Author(s)
      Ngiwsara L
    • Organizer
      26th Internal Carbohydrate Symposium
    • Place of Presentation
      Madrid City Centre (Madrid, Spain)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Molecular mechanism of glycosylases catalyzing megalosaccharide production2012

    • Author(s)
      Kim YM
    • Organizer
      BIT's 3rd Inaugural Symposium on Enzymes & Biocatalysis-2012
    • Place of Presentation
      Xian Qujiang International Conference Center (Xian, China)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Streptococcus mutans由来デキストラナーゼの結晶構造に基づく触媒機構の解明2012

    • Author(s)
      鈴木喜大
    • Organizer
      第29回PFシンポジウム
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼの合目的変異導入による生成物特異性の制御2011

    • Author(s)
      鈴木龍一郎
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼのβ→αループ2はCI-8の優先的生成に寄与する2011

    • Author(s)
      斉藤みどり
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Isomaltooligosaccharide 6-α-glucosyltransferaseサブサイト+1変異酵素の機能解析2011

    • Author(s)
      青山泰
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] アノマー反転型トレハラーゼ変異体により合成された新規非還元二糖の構造2011

    • Author(s)
      平内亨
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Production and investigation of recombinant α-glucosidase from Podospora anserina2011

    • Author(s)
      Kyung-Mo Song
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] GH family 31に属するAspergillus niger由来のα-glucosidaseの鎖長特異性の改変2011

    • Author(s)
      田上貴祥
    • Organizer
      日本応用糖質科学会平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dextran glucosidase (DexB)ウォータパス改変体の構造と機能2011

    • Author(s)
      小林桃子
    • Organizer
      日本応用糖質科学会 平成23年度大会(第60回)
    • Place of Presentation
      北大(札幌市)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] イソマルトメガロ糖とその生産酵素2011

    • Author(s)
      木村淳夫
    • Organizer
      メガロ糖・第2回シンポジウム「メガロ糖の製造と機能性ナノ材料:イソマルト型の新展開」
    • Place of Presentation
      帯畜大(帯広市)(招待講演)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular anatomy of α-1,6-glucoside-forming and -hydrolyzing enzymes, comprising megalosaccharide-producing enzyme2011

    • Author(s)
      Kim YM, Mori H, Kimura A
    • Organizer
      2011 CAB Agricultural Biotechnology Symposium on Agricultural Biomaterials for Next Generation
    • Place of Presentation
      Seoul National University (Seoul, Korea)
    • Year and Date
      2011-08-25
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular anatomy of α-1,6-glucoside(s)-forming and -hydrolyzing enzymes, comprising megalosaccharide-producing enzymes2011

    • Author(s)
      Young-Min Kim
    • Organizer
      2011 CAB Agricultural Biotechnology Symposium on "Agricultural Biomaterials for Next Generation"
    • Place of Presentation
      ソウル大学(ソウル市、韓国)(招待講演)
    • Year and Date
      2011-08-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular anatomy of α-1, 6-glucoside-forming and -hydrolyzing enzymes, compri-sing megalosaccharide-producing enzyme2011

    • Author(s)
      A.Kimura
    • Organizer
      2011 CAB Agricultural Biotechnology Symposium
    • Place of Presentation
      Seoul National University (Seoul, Korea)(招待講演)(発表確定)
    • Year and Date
      2011-08-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] GH family 31における基質の鎖長認識機構の解明2011

    • Author(s)
      田上貴祥
    • Organizer
      日本農芸化学会北海道支部夏季シンポジウム
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2011-08-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacillus circulans T-3040由来サイクロデキストラン合成酵素と環状イソマルトオリゴ糖との複合体のX線結晶構造解析2011

    • Author(s)
      鈴木喜大
    • Organizer
      第28回PFシンポジウム
    • Place of Presentation
      つくば国際会議場(つくば市)
    • Year and Date
      2011-07-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Molecular Mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production2011

    • Author(s)
      Kimura A
    • Organizer
      The 78th Annual Meeting and International Symposium in 2011 Annual Conference of Korean Society of Food Science and Technology
    • Place of Presentation
      Daegu Exhibition Convention Center (Daegu, Korea)
    • Year and Date
      2011-06-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanism of novel enzymes degrading and forming α-1,6-glucosidic linkages, including megalosaccharide production2011

    • Author(s)
      Kim YM
    • Organizer
      The 78th Annual Meeting and International Symposium in 2011 Annual Meeting of Korean Society of Food Sci ence and Technology (KoSFoST)
    • Place of Presentation
      Daegu Exhibition Convention Center(大邱広域市、韓国)(招待講演)
    • Year and Date
      2011-06-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mechanism of novel enzymes degrading and forming α-1, 6-glucosidic linkages, including megalosaccharide production2011

    • Author(s)
      A.Kimura
    • Organizer
      78th Annual Meeting and International Symposium of Korean Society of Food Science and Technology
    • Place of Presentation
      Daegu Exhibition Convention Center (Daegu, Korea)(招待講演)(発表確定)
    • Year and Date
      2011-06-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 環状イサマルトオリコ糖グルカノトランスフェラーゼの環状イソマルトオリコ糖複合体の結晶構造2011

    • Author(s)
      鈴木喜大
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 環状イソマルトオリゴ糖ルカノトランスフェラーゼにおけるCI-8の優先的生成に寄与する構造因子2011

    • Author(s)
      齊藤みどり
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bacillus circulans T-3040株由来環状イソマルトオリゴ糖グルカノトランスフェラーゼの生成物特異性の改変2011

    • Author(s)
      鈴木龍一郎
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dextran glucosidase (DexB)のウォーターパス改変酵素の機能解析2011

    • Author(s)
      小林桃子
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] ホタテガイ(Pastinopecton vessoensis)中腸腺由来<-glucosidase-C1^-による反応速度の上昇と基質特異性の変化-2011

    • Author(s)
      桝田安志
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] アノマー反転型トレハラーゼ変異体によるオリゴ糖合成2011

    • Author(s)
      平内亨
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improvement of production conditions of recombinant Podospora anserina <-glucosidase2011

    • Author(s)
      Song KM
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大(京都府)
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Enhancement of GH66 dextranase activity by truncation of N- and C-terminal variable regions, suggesting proenzyme-like activation2011

    • Author(s)
      Mori H, Nakai H, Kimura A
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Foundation (Lisbon, Portugal)
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] Structural element determining the diverse specificities for chain-length of substrate in glycoside hydrolase family 31 α-glucosidases2011

    • Author(s)
      Tagami T
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Foundation (Lisbon, Portugal)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Alteration of product specificity of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-30402011

    • Author(s)
      Suzuki R
    • Organizer
      16th European Carbohydrate Symposium
    • Place of Presentation
      Hilton Sorrento Palace Congress Centre (Naples, Italy)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dietary influence of linear α-1,6-polysaccharides on several parameters under experimental colitis depends on degree of the polymerization2011

    • Author(s)
      Hitoshi Iwaya
    • Organizer
      International Society for Nutraceuticals and Functional Foods
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enhancement of GH66 dextranase activity by truncation of N- and C-terminal variable regions, suggesting proenzyme-like activation2011

    • Author(s)
      Mori H
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Founda-tion (Lisbon, Portugal)(発表確定)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural element determining the diverse specificities for chain-length of sub-strate in glycoside hydrolase family 31 α-glucosidases2011

    • Author(s)
      Tagami T
    • Organizer
      9th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      Calouste Gulbenkian Founda-tion (Lisbon, Portugal)(発表確定)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Alteration of product specificity of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-30402011

    • Author(s)
      Suzuki R
    • Organizer
      16th European Carbohydrate Symposium
    • Place of Presentation
      Hilton Sorrento Palace Con-gress Centre (Naples, Italy)(発表確定)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structural studies of cycloisomaltooligosaccharide glucanotransferase2010

    • Author(s)
      Suzuki N
    • Organizer
      2nd International Symposium on Frontier in Agriculture Proteome Research
    • Place of Presentation
      Tsukuba NORIN (Bioscience) Hall (Tsukuba, Japan)
    • Year and Date
      2010-10-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Paenibacillus sp.598K株由来新規環状イソマルトオリゴ糖グルカノトランスフェラーゼ(CITase)の機能解析2010

    • Author(s)
      鈴木龍一郎
    • Organizer
      日本農芸化学会関東支部会
    • Place of Presentation
      千葉大学(千葉県)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bacillus circulans T-3040由来CITaseとCI8の複合体の結晶構造解析2010

    • Author(s)
      鈴木喜大
    • Organizer
      日本農芸化学会関東支部会
    • Place of Presentation
      千葉大学(千葉県)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 加水分解反応の抑制によ縮合反応の生成物を蓄積させるクルコアミラーセ変異体2010

    • Author(s)
      砂守このみ
    • Organizer
      日本農芸化学会東北支部・北海道支部合同支部大会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Oligo-1, 6-glucosidaseがC1^-依存性を示すために必要なアミノ酸残基の決定2010

    • Author(s)
      寺田智明
    • Organizer
      日本農芸化学会東北支部・北海道支部合同支部大会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acid of honeybee α-glucosidase III2010

    • Author(s)
      Ngiwsara L
    • Organizer
      4th Symposium of the α-Amylase Family
    • Place of Presentation
      Smolenice Castle (Slovakia)(招待講演)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dextran glucosidase and isomaltooligosaccharide 6-α-glucosyl transferase : mechanism of substrate recognition and transglucosvlation2010

    • Author(s)
      Mori H
    • Organizer
      4th Symposium of the α-Amylase Family
    • Place of Presentation
      Smolenice Castle (Slovakia)(招待講演)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] α-グリコシダーゼの機能と構造に関する研究2010

    • Author(s)
      奥山正幸
    • Organizer
      日本農芸化学会東北支部・北海道支部合同支部大会
    • Place of Presentation
      東北大学(宮城県)(招待講演)
    • Year and Date
      2010-09-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] 環状イソマルトオリコ糖グルカノランスフェラーセの分子解析とBacillus circulansにおける環状イソマルトオリゴ糖の新規な生合成経路の発見2010

    • Author(s)
      舟根和美
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシップ(静岡県)(招待講演)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 糖質酵素の基礎研究とその展開2010

    • Author(s)
      木村淳夫
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシップ(静岡県)(招待講演)
    • Year and Date
      2010-09-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] 酵素合成イソマルトメガロ糖のコンフォメーションと物性-その12010

    • Author(s)
      鈴木志保
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシップ(静岡県)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aspergillus niger α-glucosidaseのサブサイト+1の改変2010

    • Author(s)
      田上貴祥
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシップ(静岡県)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] isomaltooligosaccharide 6-<-glucosyltransferaseのサブサイト+1変異酵素の機能解析2010

    • Author(s)
      西村崇志
    • Organizer
      日本応用糖質科学会平成22年度大会(第59回)
    • Place of Presentation
      グランシツプ(静岡県)
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects on reactivity of honeybee α-glucosidase III mutants by site-direct mutagenesis of residues near to highly conserved region II and IV2010

    • Author(s)
      Ngiwsara L
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Effects of mutation of His-541 and Glu-542 on catalytic activities of cycloiso-maltooligosaccharide glucanotransferase from Bacillus circulars T-30402010

    • Author(s)
      Kang HK
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2010-08-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] Glycosynthase reaction catalyzed by trehalase mutant enzyme2010

    • Author(s)
      Mon H
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      幕張メッセ(千葉県)
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Shifting specificity of Aspergillus niger α-glucosidase from short chain substrates to lone chain substrates2010

    • Author(s)
      Tagami T
    • Organizer
      Plant Polysaccharide and Applied Glycoscience Workshop 2010
    • Place of Presentation
      笹川ホール(東京都)(招待講演)
    • Year and Date
      2010-07-31
    • Related Report
      2010 Annual Research Report
  • [Presentation] Biochemical characterization of a novel cycloisomaltooligosaccharide glucanotrans-ferase (CITase) from Paenibacillus sp.598K2010

    • Author(s)
      Suzuki R
    • Organizer
      Plant Polysaccharide and Applied Glycoscience Workshop 2010
    • Place of Presentation
      笹川ホール(東京都)
    • Year and Date
      2010-07-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] α-グリコシダーゼの機能と構造に関する研究2010

    • Author(s)
      奥山正幸
    • Organizer
      日本農芸化学会北海道支部会
    • Place of Presentation
      北海道大学(北海道)(招待講演)
    • Year and Date
      2010-07-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] α-グリコシダーゼの機能と構造に関する研究2010

    • Author(s)
      奥山正幸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Oligo-1,6-glucosidaseの変異導入によるCl^-イオン依存型への改変2010

    • Author(s)
      寺田智明
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 大腸菌組換えデキストリンデキストラナーゼのオリゴ糖に対する作用と触媒アミノ酸残基の解析2010

    • Author(s)
      貞廣樹里
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] GH family 31 α-グルコシダーゼにおける基質の鎖長認識機構の解明2010

    • Author(s)
      田上貴祥
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bacillus circulans T-3040株由来環状イソマルトオリゴ糖グルカノトランスフェラーゼの機能性領域の解析2010

    • Author(s)
      舟根和美
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effects of His-579 and Glu-580 on catalytic activity of cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-30402010

    • Author(s)
      Kang HK
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 環状イソマルトオリゴ糖グルカノトランスフェラーゼの結晶構造解析2010

    • Author(s)
      藤本瑞
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Putative role of Tyr227, Gln349 and Leu350 near catalytic amino acids of honeybee α-glucosidase III2010

    • Author(s)
      Mori H, Kimura A
    • Organizer
      4th Symposium on the Alpha-Amylase Family
    • Place of Presentation
      Smolenice Cattle (Smolenice, Slovakia)
    • Related Report
      2012 Final Research Report
  • [Presentation] Biochemical characterization of a novel cycloisomaltooligosaccharide glucanotransferase (CITase) from Paenibacillus sp2010

    • Author(s)
      Suzuki R, Kimura A
    • Organizer
      598K. Plant Polysaccharide and Applied Glycoscience Workshop 2010
    • Place of Presentation
      Sasagawa Hall (Tokyo, Japan)
    • Related Report
      2012 Final Research Report
  • [Presentation] オオムギβ-アミラーゼ澱粉粒への吸着および分解に関する研究2009

    • Author(s)
      原口慶子
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 糖質加水分解酵素ファミリー97酵素の配列類似性と機能類似性の関係2009

    • Author(s)
      奥山正幸
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] α-1,3グルコシド結合に高い特異性を示すα-glucosidaseの取得2009

    • Author(s)
      小林和之
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 加水分解反応の抑制によりトレハロースを縮合蓄積させるトレハラーゼ変異酵素2009

    • Author(s)
      牧孝多朗
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] アズキ発芽種子由来D-酵素(VaDPE1)のマルトオリゴ糖に対する作用と一次構造の解析2009

    • Author(s)
      田中良幸
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] 変異導入によるisomaltooligosaccharide 6-α-glucosyltransferaseの基質特異性の完全改変:α-1,6結合認識をα-1,4結合認識に変換2009

    • Author(s)
      西村崇志
    • Organizer
      日本農芸化学会北海道支部平成21年度第二回合学術講演会
    • Place of Presentation
      とかちプラザ(帯広市)
    • Year and Date
      2009-11-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Streptococcus mutans由来dextran glucosidaseの部位特異的変異導入による糖転移活性の改変(1)2009

    • Author(s)
      本同宏成
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Streptococcus mutans由来dextran glucosidaseの部位特異的変異導入による糖転移活性の改変(2)2009

    • Author(s)
      中塚大地
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] isomaltooligosaccharide 6-α-glucosyltransferaseの糖転移反応に関わる構造因子2009

    • Author(s)
      西村崇志
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Improving activity of honeybee α-glucosidase III by substitution of Q349 and L350 position2009

    • Author(s)
      Ngiwsara L
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] テンサイα-glucosidaseのサブサイト+2および+3の形跡に重要なアミノ酸残基の決定2009

    • Author(s)
      田上貴祥
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Bacteroides thetaiotaomicron由来SusBの触媒反応におけるCa^<2+>の役割2009

    • Author(s)
      吉田拓弥
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Preliminary structure determination of polysaccharide extract from Rhizobium sp.2009

    • Author(s)
      Wongchawalit J
    • Organizer
      平成21年度日本応用糖質科学会大会(第58回)
    • Place of Presentation
      弘前大学(弘前市)
    • Year and Date
      2009-09-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] Preliminary structure determination of water insoluble polysaccharide extracted from soil bacteria2009

    • Author(s)
      Wongchawalit J, Hashidoko Y, Okuyama M, Mori H, Chiba S, Kimura A
    • Organizer
      8th Carbohydrate Bioengineering Meeting
    • Place of Presentation
      2009 Hotel Continental Terme and Congress Hall (Naples, Italy)
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/top-page-jpn.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/top-page-jpn.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://hecate.general.hokudai.ac.jp/welcome/top-page-jpn.html

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

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