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東南アジアにおけるダニ媒介性動物原虫感染症の流行実態の解明と予防対策の確立

Research Project

Project/Area Number 21405036
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

玄 学南  帯広畜産大学, 原虫病研究センター, 教授 (10292096)

Co-Investigator(Kenkyū-buntansha) 山岸 潤也  帯広畜産大学, 原虫病研究センター, 研究員 (80535328)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2010: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2009: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
KeywordsBabesia / Theileria / マダニ / 東南アジア / 疫学調査 / 遺伝子診断 / 血清学診断 / 予防対策 / マダニ媒介
Research Abstract

本研究は東南アジアにおけるダニ媒介性動物原虫感染症の流行実態の解明と予防対策の確立を目指して実施する。本年度に実施した研究内容と得られた研究成果は以下の通りである。
1. 昨年度に引き続きタイ国北部地域における牛のマダニ媒介性原虫感染症の流行実態を調べた。計200頭の牛の血液サンプルを採集し、全DNAを抽出した。ウシバベシア原虫特異PCR法を用いて、血液サンプル中の原虫DNAの検出を行ったところ、Babesia bovisとBabesia bigeminaの陽性率がそれぞれ12%(24/200)と21%(42/200)であった。この結果より、ウシバベシア症はタイ国北部地域広く流行しており、本症の制圧対策は当地域の牛の生産性向上に重要であることが示唆された。
2. フィリピンマニラ周辺地域における牛のマダニ媒介性原虫感染症の流行実態を調べた。計250頭の牛より血液サンプルを採集し、全DNAを抽出した。ウシバベシア原虫特異PCR法とウシタイレリア原虫特異PCR法を用いて予備実験を行ったところ、2種類のウシバベシア原虫(Babesia bovisとBabesia bigemina)と1種類のウシタイレリア原虫(Theileria orientalis)DNAが高率に検出された。これらの結果により、フィリピンマニラ周辺地域の牛にはマダニ媒介性バベシア原虫とタイレリア原虫感染症が高率に流行していることが示唆された。

Report

(2 results)
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] Molecular and serological prevalence of Babesia bovis and Babesia bigemina in water buffaloes in the northeast region of Thailand.2011

    • Author(s)
      Terkawi, et al.
    • Journal Title

      Vet.Parasitol.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiological survey of Theileria orientalis in Thua Thien Hue Province, Vietnam.2011

    • Author(s)
      Khukhuu, et al.
    • Journal Title

      J.Vet.Med.Sci.

      Volume: (印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seroprevalence of Babesia infections of dairy cows in northern Thailand.2010

    • Author(s)
      Iseki, et al.
    • Journal Title

      Vet.Parasitol.

      Volume: 170 Pages: 193-196

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Artesunate, a potential drug for treatment of Babesia infection.2010

    • Author(s)
      Goo, et al.
    • Journal Title

      Parasitol.Int.

      Volume: 59 Pages: 481-486

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Serodiagnosis of Toxoplasma gondii infection in dairy cows in Thailand.2010

    • Author(s)
      Inpankaew, et al.
    • Journal Title

      Transbound.Emerg.Dis.

      Volume: 57 Pages: 42-45

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of two selected antigen loci in Babesia gibsoni Asian genotype obtained from Japan and Jeju island of South Korea.2009

    • Author(s)
      Jia, et al.
    • Journal Title

      Vet.Parasitol. 162

      Pages: 142-146

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seroprevalence of Cryptosporidium parvum infection of dairy cows in three northern provinces of Thailand determined by enzyme-linked immunosorbent assay using recombinant antigen CpP23.2009

    • Author(s)
      Inpankaew, et al.
    • Journal Title

      Onderstepoort J.Vet. Res. 76

      Pages: 161-165

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cloning and molecular characterization of tick kynurenin aminotransferase(HlKAT)from Haemaphysalis longicornis(Atari : Ixodidae).2009

    • Author(s)
      Battsetseg, et al.
    • Journal Title

      Parasitol. Res 105

      Pages: 669-679

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and immunological characterization of Babesia gibsoni and B.microti heat shock protein-70.2009

    • Author(s)
      Terkawi, et al.
    • Journal Title

      Parasite Immunol. 31

      Pages: 328-340

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of an intracellular cystatin homolog from the tick Haemaphysalis longicornis.2009

    • Author(s)
      Zhou, et al.
    • Journal Title

      Vet.Parasitol. 160

      Pages: 180-183

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Aldolase forms a bridge between the actin cytoskeleton and TRAP cytoplasmic tail in Babesia gibsoni.2010

    • Author(s)
      Goo, et al.
    • Organizer
      第150回日本獣医学会
    • Place of Presentation
      帯広畜産大学
    • Year and Date
      2010-09-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Molecular characterization of a new spherical body protein of Babesia bovis and evaluation its potential use for serodiagnosis.2009

    • Author(s)
      Terkawi, et al.
    • Organizer
      第148回日本獣医学会
    • Place of Presentation
      鳥取大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.obihiro.ac.jp/~geneticbiochem/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.obihiro.ac.jp/~geneticbiochem/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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