Exploration of novel metal-metabolizing bacterium to develop the purification of rare metal pollution and recovery system of rare metal

• Project/Area Number: 21405038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Boundary agriculture
• Research Institution: Kyoto University
• Principal Investigator: KAWAMOTO Jun 京都大学, 化学研究所, 助教 (90511238)
• Co-Investigator(Kenkyū-buntansha): KURIHARA Tatsuo 京都大学, 化学研究所, 教授 (70243087)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000); Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
• Keywords: バイオテクノロジー / 応用微生物 / 生体機能利用 / 微生物機能 / 環境浄化 / 金属呼吸 / 環境技術 / 微生物 / 金属代謝

Research Abstract

To develop the microbial purification and recovery system for rare metal, we explored novel metal metabolizing bacteria from cold environments, such as grass area in Inner Mongolia, China, and glacier area in Austria, and analyzed metal respiration mechanism of a metal-reducing bacterium, Shewanella livingstonensis Ac10. We successfully obtained various cold-adapted stains, and application of these strains is expected to develop the soil purification and rare metal-recovery system at cold environments. We also identified that S. livingstonensis Ac10, a cold-adapted metal-reducing bacterium, inducibly synthesized a specific channel proteins in the presence of trivalent iron, which is involved in iron transport during its iron respiration.

Research Products

• [Journal Article] Occurrence of bacterial membrane microdomain at the cell division site enriched in phospholipids with polyunsaturated hydrocarbon chain. (2012)
  • Author(s): Sato, S., Kawamoto, J., Sato, S.B., Watanabe, B., Hiratake, J., Esaki, N., and Kurihara, T.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 29 Pages: 24113-21
  • DOI: 10.1074/jbc.m111.318311
  • NAID: 120005302081
  • Peer Reviewed
• [Journal Article] dentification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis. (2012)
  • Author(s): Park, J., Kawamoto, J., Esaki, N., and Kurihara, T.
  • Journal Title: Extremophiles Volume: 16 Issue: 2 Pages: 227-236
  • DOI: 10.1007/s00792-011-0422-z
  • Peer Reviewed
• [Journal Article] Function of FADH2-dependent 2-haloacrylate hydratase from a 2-chloroacrylate-utilizing bacterium, Burkholderia sp (2011)
  • Author(s): WS., Vasudevan, A., Fujita, M., Kurata, A., Kawamoto, J., Esaki, N., and Kurihara, T
  • Journal Title: Trace Nutrients Research Volume: 28 Pages: 58-64
  • URL: http://www.jtnrs.com/sym28/P-08.pdf
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid plays a role in stabilizing dynamic membrane structure in the deep-sea piezophile Shewanella violacea: a study employing high-pressure time-resolved fluorescence anisotropy measurement (2011)
  • Author(s): Usui, K., Hiraki, T., Kawamoto, J., Kurihara, T., Nogi, Y., Kato, C., and Abe, F.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1818 Issue: 3 Pages: 574-583
  • DOI: 10.1016/j.bbamem.2011.10.010
  • Peer Reviewed
• [Journal Article] Piezotolerance of the Respiratory Terminal Oxidase Activity of the Piezophilic Shewanella violacea DSS12 as Compared with Non-Piezophilic Shewanella Specie (2011)
  • Author(s): Tamegai, H., Ota, Y., Haga, M., Fujimori, H., Kato, C., Nogi, Y., Kawamoto, J., Kurihara, T., and Sambongi, Y
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 75 Pages: 919-924
  • NAID: 10028272647
  • URL: http://dx.doi.org/10.1271/bbb.100882
  • Peer Reviewed
• [Journal Article] Differential roles of internal and terminal double bonds in docosahexaenoic acid: Comparative study of cytotoxicity of polyunsaturated fatty acids to HT-29 human colorectal tumor cell line (2011)
  • Author(s): Sato, S.B., Sato, S., Kawamoto, J., and Kurihara, T.
  • Journal Title: Prostaglandins Leukot Essent Fatty Acids Volume: 84 Issue: 1-2 Pages: 31-37
  • DOI: 10.1016/j.plefa.2010.09.006
  • Peer Reviewed
• [Journal Article] Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety (2011)
  • Author(s): Omori, T., Honda, A., Mihara, H., Kurihara, T., and Esaki, N
  • Journal Title: Journal of Chromatography B Volume: 879 Issue: 29 Pages: 3296-3302
  • DOI: 10.1016/j.jchromb.2011.04.033
  • NAID: 120003644380
  • Peer Reviewed
• [Journal Article] Thermal Stability of Cytochrome c5 of Pressure-Sensitive Shewanella livingstonensis (2011)
  • Author(s): Masanari, M., Wakai, S., Tamegai, H.,Kurihara, T., Kato, C., and Sambogi, Y.
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 75 Pages: 1859-1861
  • URL: https://www.jstage.jst.go.jp/article/bbb/75/9/75_110370/_pdf
  • Peer Reviewed
• [Journal Article] Mammalian Selenocysteine Lyase Is Involved In Selenoprotein Biosynthesis (2011)
  • Author(s): Kurokawa, S., Takehashi, M., Tanaka, H., Mihara, H., Kurihara, T., Tanaka, S., Hill, K., Burk, R., and Esaki, N.
  • Journal Title: Journal of Nutritional Science and Vitaminology Volume: 57 Pages: 298-305
  • NAID: 130001373002
  • URL: http://dx.doi.org/10.3177/jnsv.57.298
  • Peer Reviewed
• [Journal Article] A mechanistic analysis of enzymatic degradation of organohalogen compounds (2011)
  • Author(s): Kurihara, T.,Biosci
  • Journal Title: Biotechnol Biochem Volume: 75 Pages: 189-198
  • NAID: 10027897237
  • URL: http://dx.doi.org/10.1271/bbb.100746
  • Peer Reviewed
• [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures (2011)
  • Author(s): Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N., and Kurihara, T
  • Journal Title: Environmental Microbiology Volume: 13 Issue: 8 Pages: 2293-2298
  • DOI: 10.1111/j.1462-2920.2011.02487.x
  • Peer Reviewed
• [Journal Article] Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil. (2011)
  • Author(s): Hidese, R., Mihara, H., Kurihara, T., and Esaki, N.
  • Journal Title: J Bacteriol Volume: 193 Issue: 4 Pages: 989-993
  • DOI: 10.1128/jb.01178-10
  • Peer Reviewed
• [Journal Article] In vitro Refolding of an OmpA Homolog, a Major Cold-Inducible Outer Membrane Protein, from a Psychrotrophic Bacterium, Shewanella livingstonensis Ac10., Dai, X.-z. (2011)
  • Author(s): Kawamoto, J., Esaki, N., and Kurihara, T
  • Journal Title: Journal of Japanese Society for Extremophiles Volume: 10 Pages: 90-96
  • URL: http://jglobal.jst.go.jp/public/20090422/201202222875433695
  • Peer Reviewed
• [Journal Article] 好冷性細菌が生産する高度不飽和脂肪酸含有リン脂質の生理機能 (2011)
  • Author(s): 川本 純, 栗原達夫
  • Journal Title: バイオサイエンスとインダストリー Volume: 69 Pages: 305-307
  • NAID: 10029237036
• [Journal Article] The distribution of phosphatidyl-D-serine in the rat. (2010)
  • Author(s): T. Omori, H. Mihara, T. Kurihara, and N. Esaki
  • Journal Title: Biosci Biotechnol Biochem Volume: 74 Pages: 1953-1955
  • NAID: 10027559956
  • URL: http://dx.doi.org/10.1271/bbb.100271
  • Peer Reviewed
• [Journal Article] IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli (2010)
  • Author(s): W. Zhang, A. Urban, H. Mihara, S. Leimkuhler, T. Kurihara, and N. Esaki.
  • Journal Title: J Biol Chem Volume: 285 Issue: 4 Pages: 2302-2308
  • DOI: 10.1074/jbc.m109.082172
  • Peer Reviewed
• [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase. (2010)
  • Author(s): R. Omi, S. Kurokawa, H. Mihara, H. Hayashi, M. Goto, I. Miyahara, T. Kurihara, K. Hirotsu and N. Esaki.
  • Journal Title: J Biol Chem Volume: 285 Issue: 16 Pages: 12133-12139
  • DOI: 10.1074/jbc.m109.084475
  • Peer Reviewed
• [Journal Article] 2-Haloacrylate hydratase, a new class of flavoenzyme that catalyzes the addition of water to the substrate for dehalogenation (2010)
  • Author(s): A. M. Mowafy, T. Kurihara, A. Kurata, T. Uemura and N. Esaki
  • Journal Title: Appl Environ Microbiol Volume: 76 Issue: 18 Pages: 6032-6037
  • DOI: 10.1128/aem.00334-10
  • Peer Reviewed
• [Journal Article] 低温細菌における長鎖高度不飽和脂肪酸の生合成と機能 (2010)
  • Author(s): 栗原達夫, 川本純, 江崎信芳.
  • Journal Title: 蛋白質核酸酵素 Volume: 55 Pages: 94-99
  • NAID: 40016985470
  • URL: http://www.jbsoc.or.jp/event/magazine/pdf/81-08-08.pdf
• [Journal Article] 好冷酵素の特性と利用法 (2010)
  • Author(s): 栗原達夫
  • Journal Title: 酵素利用技術大系 Pages: 239-243
• [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase (2010)
  • Author(s): Omi R, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 12133-9
  • Peer Reviewed
• [Journal Article] IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli (2010)
  • Author(s): Zhang W, et al
  • Journal Title: J Biol Chem Volume: 285 Pages: 2302-8
  • Peer Reviewed
• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium (2009)
  • Author(s): Shewanella livingstonensis Ac10. J. Kawamoto, T.Kurihara, K. Yamamoto, M. Nagayasu, Y. Tani, H. Mihara, M. Hosokawa, T. Baba, S. B. Sato and N. Esaki.
  • Journal Title: J Bacteriol Volume: 191 Issue: 2 Pages: 632-40
  • DOI: 10.1128/jb.00881-08
  • Peer Reviewed
• [Journal Article] X-Ray crystallographic and mutational studies of fluoroacetate dehalogenase from Burkholderia sp. (2009)
  • Author(s): strain FA1. K. Jitsumori, R. Omi, T. Kurihara, A. Kurata, H. Mihara, I. Miyahara, K. Hirotsu and N. Esaki
  • Journal Title: J Bacteriol Volume: 191 Issue: 8 Pages: 2630-7
  • DOI: 10.1128/jb.01654-08
  • Peer Reviewed
• [Journal Article] Crystal structure of a homolog of mammalian serine racemase from Schizosaccharomyces pombe (2009)
  • Author(s): M. Goto, T. Yamauchi, N. Kamiya, I. Miyahara, T. Yoshimura, H. Mihara, T. Kurihara, K. Hirotsu and N. Esaki
  • Journal Title: J Biol Chem Volume: 284 Issue: 38 Pages: 25944-52
  • DOI: 10.1074/jbc.m109.010470
  • Peer Reviewed
• [Journal Article] Occurrence of phosphatidyl-D-serine in the rat cerebrum (2009)
  • Author(s): T. Omori, H. Mihara, T. Kurihara, and N. Esaki
  • Journal Title: Biochem Biophys Res Commun Volume: 382 Issue: 2 Pages: 415-8
  • DOI: 10.1016/j.bbrc.2009.03.035
  • Peer Reviewed
• [Journal Article] Serine racemase with catalytically active lysinoalanyl residue. (2009)
  • Author(s): T. Yamauchi, M. Goto, H. Y. Wu, T. Uo, T. Yoshimura, H. Mihara, T. Kurihara, I. Miyahara, K. Hirotsu and N. Esaki
  • Journal Title: J Biochem Volume: 145 Issue: 4 Pages: 421-4
  • DOI: 10.1093/jb/mvp010
  • NAID: 10026301897
  • Peer Reviewed
• [Journal Article] Possible role of red blood cells in selenocysteine metabolism. T. Imai, H. Mihara (2009)
  • Author(s): T. Kurihara, and N. Esaki
  • Journal Title: Trace Nutrients Research Volume: 26 Pages: 22-25
  • URL: http://ww.jtnrs.com/sym26/26_022.pdf
  • Peer Reviewed
• [Journal Article] Selenocysteine Is Selectively Taken Up by Red Blood Cells (2009)
  • Author(s): T. Imai, H. Mihara, T. Kurihara,and N. Esaki
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 73 Issue: 12 Pages: 2746-2748
  • DOI: 10.1271/bbb.90306
  • NAID: 10027549360
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] 低温下で機能する酵素 (2009)
  • Author(s): 栗原達夫, 江崎信芳
  • Journal Title: 化学工学 Volume: 73 Pages: 324-327
  • NAID: 10026200625
• [Journal Article] 低温細菌における高度不飽和脂肪酸含有リン脂質の機能 (2009)
  • Author(s): 栗原達夫
  • Journal Title: 生化学 Volume: 81 Pages: 716-719
  • NAID: 10026298336
  • URL: http://www.jbsoc.or.jp/event/magazine/pdf/81-08-08.pdf
• [Journal Article] 好冷性細菌の低温適応に関わるタンパク質とリン脂質 (2009)
  • Author(s): 栗原達夫, 川本純, 江崎信芳
  • Journal Title: 生化学 Volume: 81 Pages: 1072-1079
  • NAID: 10026299872
  • URL: http://www.jbsoc.or.jp/event/magazine/pdf/81-12-07.pd
• [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium Shewanella livingstonensis Ac10. (2009)
  • Author(s): Kawamoto J, Kurihara T, Yamamoto K, Nagayasu M, Tani Y, Mihara H, Hosokawa M, Baba T, Sato SB, Esaki N.
  • Journal Title: J Bacteriol. Volume: 191 Pages: 632-640
  • Peer Reviewed
• [Journal Article] 細胞がセレンをタンパク質に取り込む機構の解析とその応用 (2009)
  • Author(s): 栗原達夫, 三原久明, 江崎信芳
  • Journal Title: 環境・資源保全のためのメタルバイオテクノロジー Pages: 241-246
• [Presentation] Cold-adaptation mechanism od an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac10 (2011)
  • Author(s): Jun Kawamoto
  • Organizer: 4thinternational conference on Polar and Alpine Microbiology
  • Place of Presentation: Ljubljana, Slovenia
  • Year and Date: 2011-09-05
• [Presentation] Cold-adaptation mechanism of a psychrotrophic bacterium, Shewanella livingstonesis Ac10 (2010)
  • Author(s): Jun Kawamoto
  • Organizer: MicroPerm workshop
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2010-11-08
• [Presentation] Cold-adaptation mechanism of a psychrotrophic bacterium, Shewanella livingstonesis Ac10 (2010)
  • Author(s): Jun Kawamoto, Tatsuo Kurihara
  • Organizer: MicroPerm workshop, circumpolar integration of permafrost micobiological studies
  • Place of Presentation: Potsdam, Germany
  • Year and Date: 2010-11-08
• [Presentation] 低温誘導性ポーリンのフォールディングにおけるエイコサペンタエン酸含有リン脂質の生理的役割 (2009)
  • Author(s): 川本純、代先祝、佐藤智、栗原達夫、江崎信芳
  • Organizer: 第10回極限環境微生物学会
  • Place of Presentation: 明治大学駿河台キャンパス
  • Year and Date: 2009-10-28
• [Presentation] Eicosapentaenoic acid plays an important role in assembly of cell division machinery of an Antarctic sea bacterium, Shewanella livingstonensis Ac10 (2009)
  • Author(s): Jun Kawamoto
  • Organizer: Xth SCAR International Biology Symposium
  • Place of Presentation: 札幌
  • Year and Date: 2009-07-26