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Exploration of novel metal-metabolizing bacterium to develop the purification of rare metal pollution and recovery system of rare metal

Research Project

Project/Area Number 21405038
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Boundary agriculture
Research InstitutionKyoto University

Principal Investigator

KAWAMOTO Jun  京都大学, 化学研究所, 助教 (90511238)

Co-Investigator(Kenkyū-buntansha) KURIHARA Tatsuo  京都大学, 化学研究所, 教授 (70243087)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2009: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Keywordsバイオテクノロジー / 応用微生物 / 生体機能利用 / 微生物機能 / 環境浄化 / 金属呼吸 / 環境技術 / 微生物 / 金属代謝
Research Abstract

To develop the microbial purification and recovery system for rare metal, we explored novel metal metabolizing bacteria from cold environments, such as grass area in Inner Mongolia, China, and glacier area in Austria, and analyzed metal respiration mechanism of a metal-reducing bacterium, Shewanella livingstonensis Ac10. We successfully obtained various cold-adapted stains, and application of these strains is expected to develop the soil purification and rare metal-recovery system at cold environments. We also identified that S. livingstonensis Ac10, a cold-adapted metal-reducing bacterium, inducibly synthesized a specific channel proteins in the presence of trivalent iron, which is involved in iron transport during its iron respiration.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (39 results)

All 2012 2011 2010 2009

All Journal Article (34 results) (of which Peer Reviewed: 27 results) Presentation (5 results)

  • [Journal Article] Occurrence of bacterial membrane microdomain at the cell division site enriched in phospholipids with polyunsaturated hydrocarbon chain.2012

    • Author(s)
      Sato, S., Kawamoto, J., Sato, S.B., Watanabe, B., Hiratake, J., Esaki, N., and Kurihara, T.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 29 Pages: 24113-21

    • DOI

      10.1074/jbc.m111.318311

    • NAID

      120005302081

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] dentification of cold-inducible inner membrane proteins of the psychrotrophic bacterium, Shewanella livingstonensis Ac10, by proteomic analysis.2012

    • Author(s)
      Park, J., Kawamoto, J., Esaki, N., and Kurihara, T.
    • Journal Title

      Extremophiles

      Volume: 16 Issue: 2 Pages: 227-236

    • DOI

      10.1007/s00792-011-0422-z

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Function of FADH2-dependent 2-haloacrylate hydratase from a 2-chloroacrylate-utilizing bacterium, Burkholderia sp2011

    • Author(s)
      WS., Vasudevan, A., Fujita, M., Kurata, A., Kawamoto, J., Esaki, N., and Kurihara, T
    • Journal Title

      Trace Nutrients Research

      Volume: 28 Pages: 58-64

    • URL

      http://www.jtnrs.com/sym28/P-08.pdf

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Eicosapentaenoic acid plays a role in stabilizing dynamic membrane structure in the deep-sea piezophile Shewanella violacea: a study employing high-pressure time-resolved fluorescence anisotropy measurement2011

    • Author(s)
      Usui, K., Hiraki, T., Kawamoto, J., Kurihara, T., Nogi, Y., Kato, C., and Abe, F.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1818 Issue: 3 Pages: 574-583

    • DOI

      10.1016/j.bbamem.2011.10.010

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Piezotolerance of the Respiratory Terminal Oxidase Activity of the Piezophilic Shewanella violacea DSS12 as Compared with Non-Piezophilic Shewanella Specie2011

    • Author(s)
      Tamegai, H., Ota, Y., Haga, M., Fujimori, H., Kato, C., Nogi, Y., Kawamoto, J., Kurihara, T., and Sambongi, Y
    • Journal Title

      Bioscience Biotechnology and Biochemistry

      Volume: 75 Pages: 919-924

    • NAID

      10028272647

    • URL

      http://dx.doi.org/10.1271/bbb.100882

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential roles of internal and terminal double bonds in docosahexaenoic acid: Comparative study of cytotoxicity of polyunsaturated fatty acids to HT-29 human colorectal tumor cell line2011

    • Author(s)
      Sato, S.B., Sato, S., Kawamoto, J., and Kurihara, T.
    • Journal Title

      Prostaglandins Leukot Essent Fatty Acids

      Volume: 84 Issue: 1-2 Pages: 31-37

    • DOI

      10.1016/j.plefa.2010.09.006

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of novel mammalian phospholipids containing threonine, aspartate, and glutamate as the base moiety2011

    • Author(s)
      Omori, T., Honda, A., Mihara, H., Kurihara, T., and Esaki, N
    • Journal Title

      Journal of Chromatography B

      Volume: 879 Issue: 29 Pages: 3296-3302

    • DOI

      10.1016/j.jchromb.2011.04.033

    • NAID

      120003644380

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Thermal Stability of Cytochrome c5 of Pressure-Sensitive Shewanella livingstonensis2011

    • Author(s)
      Masanari, M., Wakai, S., Tamegai, H.,Kurihara, T., Kato, C., and Sambogi, Y.
    • Journal Title

      Bioscience Biotechnology and Biochemistry

      Volume: 75 Pages: 1859-1861

    • URL

      https://www.jstage.jst.go.jp/article/bbb/75/9/75_110370/_pdf

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mammalian Selenocysteine Lyase Is Involved In Selenoprotein Biosynthesis2011

    • Author(s)
      Kurokawa, S., Takehashi, M., Tanaka, H., Mihara, H., Kurihara, T., Tanaka, S., Hill, K., Burk, R., and Esaki, N.
    • Journal Title

      Journal of Nutritional Science and Vitaminology

      Volume: 57 Pages: 298-305

    • NAID

      130001373002

    • URL

      http://dx.doi.org/10.3177/jnsv.57.298

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A mechanistic analysis of enzymatic degradation of organohalogen compounds2011

    • Author(s)
      Kurihara, T.,Biosci
    • Journal Title

      Biotechnol Biochem

      Volume: 75 Pages: 189-198

    • NAID

      10027897237

    • URL

      http://dx.doi.org/10.1271/bbb.100746

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Favourable effects of eicosapentaenoic acid on the late step of the cell division in a piezophilic bacterium, Shewanella violacea DSS12, at high-hydrostatic pressures2011

    • Author(s)
      Kawamoto, J., Sato, T., Nakasone, K., Kato, C., Mihara, H., Esaki, N., and Kurihara, T
    • Journal Title

      Environmental Microbiology

      Volume: 13 Issue: 8 Pages: 2293-2298

    • DOI

      10.1111/j.1462-2920.2011.02487.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Escherichia coli dihydropyrimidine dehydrogenase is a novel NAD-dependent heterotetramer essential for the production of 5,6-dihydrouracil.2011

    • Author(s)
      Hidese, R., Mihara, H., Kurihara, T., and Esaki, N.
    • Journal Title

      J Bacteriol

      Volume: 193 Issue: 4 Pages: 989-993

    • DOI

      10.1128/jb.01178-10

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vitro Refolding of an OmpA Homolog, a Major Cold-Inducible Outer Membrane Protein, from a Psychrotrophic Bacterium, Shewanella livingstonensis Ac10., Dai, X.-z.2011

    • Author(s)
      Kawamoto, J., Esaki, N., and Kurihara, T
    • Journal Title

      Journal of Japanese Society for Extremophiles

      Volume: 10 Pages: 90-96

    • URL

      http://jglobal.jst.go.jp/public/20090422/201202222875433695

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 好冷性細菌が生産する高度不飽和脂肪酸含有リン脂質の生理機能2011

    • Author(s)
      川本 純, 栗原達夫
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 69 Pages: 305-307

    • NAID

      10029237036

    • Related Report
      2011 Final Research Report
  • [Journal Article] The distribution of phosphatidyl-D-serine in the rat.2010

    • Author(s)
      T. Omori, H. Mihara, T. Kurihara, and N. Esaki
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 74 Pages: 1953-1955

    • NAID

      10027559956

    • URL

      http://dx.doi.org/10.1271/bbb.100271

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli2010

    • Author(s)
      W. Zhang, A. Urban, H. Mihara, S. Leimkuhler, T. Kurihara, and N. Esaki.
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 4 Pages: 2302-2308

    • DOI

      10.1074/jbc.m109.082172

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase.2010

    • Author(s)
      R. Omi, S. Kurokawa, H. Mihara, H. Hayashi, M. Goto, I. Miyahara, T. Kurihara, K. Hirotsu and N. Esaki.
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 16 Pages: 12133-12139

    • DOI

      10.1074/jbc.m109.084475

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 2-Haloacrylate hydratase, a new class of flavoenzyme that catalyzes the addition of water to the substrate for dehalogenation2010

    • Author(s)
      A. M. Mowafy, T. Kurihara, A. Kurata, T. Uemura and N. Esaki
    • Journal Title

      Appl Environ Microbiol

      Volume: 76 Issue: 18 Pages: 6032-6037

    • DOI

      10.1128/aem.00334-10

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 低温細菌における長鎖高度不飽和脂肪酸の生合成と機能2010

    • Author(s)
      栗原達夫, 川本純, 江崎信芳.
    • Journal Title

      蛋白質核酸酵素

      Volume: 55 Pages: 94-99

    • NAID

      40016985470

    • URL

      http://www.jbsoc.or.jp/event/magazine/pdf/81-08-08.pdf

    • Related Report
      2011 Final Research Report
  • [Journal Article] 好冷酵素の特性と利用法2010

    • Author(s)
      栗原達夫
    • Journal Title

      酵素利用技術大系

      Pages: 239-243

    • Related Report
      2011 Final Research Report
  • [Journal Article] Reaction mechanism and molecular basis for selenium/sulfur discrimination of selenocysteine lyase2010

    • Author(s)
      Omi R, et al
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 12133-9

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli2010

    • Author(s)
      Zhang W, et al
    • Journal Title

      J Biol Chem

      Volume: 285 Pages: 2302-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium2009

    • Author(s)
      Shewanella livingstonensis Ac10. J. Kawamoto, T.Kurihara, K. Yamamoto, M. Nagayasu, Y. Tani, H. Mihara, M. Hosokawa, T. Baba, S. B. Sato and N. Esaki.
    • Journal Title

      J Bacteriol

      Volume: 191 Issue: 2 Pages: 632-40

    • DOI

      10.1128/jb.00881-08

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-Ray crystallographic and mutational studies of fluoroacetate dehalogenase from Burkholderia sp.2009

    • Author(s)
      strain FA1. K. Jitsumori, R. Omi, T. Kurihara, A. Kurata, H. Mihara, I. Miyahara, K. Hirotsu and N. Esaki
    • Journal Title

      J Bacteriol

      Volume: 191 Issue: 8 Pages: 2630-7

    • DOI

      10.1128/jb.01654-08

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a homolog of mammalian serine racemase from Schizosaccharomyces pombe2009

    • Author(s)
      M. Goto, T. Yamauchi, N. Kamiya, I. Miyahara, T. Yoshimura, H. Mihara, T. Kurihara, K. Hirotsu and N. Esaki
    • Journal Title

      J Biol Chem

      Volume: 284 Issue: 38 Pages: 25944-52

    • DOI

      10.1074/jbc.m109.010470

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of phosphatidyl-D-serine in the rat cerebrum2009

    • Author(s)
      T. Omori, H. Mihara, T. Kurihara, and N. Esaki
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 382 Issue: 2 Pages: 415-8

    • DOI

      10.1016/j.bbrc.2009.03.035

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serine racemase with catalytically active lysinoalanyl residue.2009

    • Author(s)
      T. Yamauchi, M. Goto, H. Y. Wu, T. Uo, T. Yoshimura, H. Mihara, T. Kurihara, I. Miyahara, K. Hirotsu and N. Esaki
    • Journal Title

      J Biochem

      Volume: 145 Issue: 4 Pages: 421-4

    • DOI

      10.1093/jb/mvp010

    • NAID

      10026301897

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Possible role of red blood cells in selenocysteine metabolism. T. Imai, H. Mihara2009

    • Author(s)
      T. Kurihara, and N. Esaki
    • Journal Title

      Trace Nutrients Research

      Volume: 26 Pages: 22-25

    • URL

      http://ww.jtnrs.com/sym26/26_022.pdf

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Selenocysteine Is Selectively Taken Up by Red Blood Cells2009

    • Author(s)
      T. Imai, H. Mihara, T. Kurihara,and N. Esaki
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 73 Issue: 12 Pages: 2746-2748

    • DOI

      10.1271/bbb.90306

    • NAID

      10027549360

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 低温下で機能する酵素2009

    • Author(s)
      栗原達夫, 江崎信芳
    • Journal Title

      化学工学

      Volume: 73 Pages: 324-327

    • NAID

      10026200625

    • Related Report
      2011 Final Research Report
  • [Journal Article] 低温細菌における高度不飽和脂肪酸含有リン脂質の機能2009

    • Author(s)
      栗原達夫
    • Journal Title

      生化学

      Volume: 81 Pages: 716-719

    • NAID

      10026298336

    • URL

      http://www.jbsoc.or.jp/event/magazine/pdf/81-08-08.pdf

    • Related Report
      2011 Final Research Report
  • [Journal Article] 好冷性細菌の低温適応に関わるタンパク質とリン脂質2009

    • Author(s)
      栗原達夫, 川本純, 江崎信芳
    • Journal Title

      生化学

      Volume: 81 Pages: 1072-1079

    • NAID

      10026299872

    • URL

      http://www.jbsoc.or.jp/event/magazine/pdf/81-12-07.pd

    • Related Report
      2011 Final Research Report
  • [Journal Article] Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium Shewanella livingstonensis Ac10.2009

    • Author(s)
      Kawamoto J, Kurihara T, Yamamoto K, Nagayasu M, Tani Y, Mihara H, Hosokawa M, Baba T, Sato SB, Esaki N.
    • Journal Title

      J Bacteriol.

      Volume: 191 Pages: 632-640

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞がセレンをタンパク質に取り込む機構の解析とその応用2009

    • Author(s)
      栗原達夫, 三原久明, 江崎信芳
    • Journal Title

      環境・資源保全のためのメタルバイオテクノロジー

      Pages: 241-246

    • Related Report
      2009 Annual Research Report
  • [Presentation] Cold-adaptation mechanism od an Antarctic psychrotrophic bacterium, Shewanella livingstonensis Ac102011

    • Author(s)
      Jun Kawamoto
    • Organizer
      4thinternational conference on Polar and Alpine Microbiology
    • Place of Presentation
      Ljubljana, Slovenia
    • Year and Date
      2011-09-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Cold-adaptation mechanism of a psychrotrophic bacterium, Shewanella livingstonesis Ac102010

    • Author(s)
      Jun Kawamoto
    • Organizer
      MicroPerm workshop
    • Place of Presentation
      Potsdam, Germany
    • Year and Date
      2010-11-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Cold-adaptation mechanism of a psychrotrophic bacterium, Shewanella livingstonesis Ac102010

    • Author(s)
      Jun Kawamoto, Tatsuo Kurihara
    • Organizer
      MicroPerm workshop, circumpolar integration of permafrost micobiological studies
    • Place of Presentation
      Potsdam, Germany
    • Year and Date
      2010-11-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低温誘導性ポーリンのフォールディングにおけるエイコサペンタエン酸含有リン脂質の生理的役割2009

    • Author(s)
      川本純、代先祝、佐藤智、栗原達夫、江崎信芳
    • Organizer
      第10回極限環境微生物学会
    • Place of Presentation
      明治大学駿河台キャンパス
    • Year and Date
      2009-10-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Eicosapentaenoic acid plays an important role in assembly of cell division machinery of an Antarctic sea bacterium, Shewanella livingstonensis Ac102009

    • Author(s)
      Jun Kawamoto
    • Organizer
      Xth SCAR International Biology Symposium
    • Place of Presentation
      札幌
    • Year and Date
      2009-07-26
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2023-03-16  

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