Development of novel malaria transmission blocking vaccine

• Project/Area Number: 21406010
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Ehime University
• Principal Investigator: TORII Motomi 愛媛大学, 大学院・医学系研究科, 教授 (20164072)
• Co-Investigator(Kenkyū-buntansha): MAYUMI Tachibana 愛媛大学, 大学院医学系研究科, 助教 (00301325)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
• Keywords: マラリア / ワクチン / 伝搬阻止 / 原虫 / 組換えタンパク質

Research Abstract

The aim of the malaria transmission-blocking vaccine(TBV) is to block the development of malaria parasites in the mosquito and prevent the following infection to the host. We focused on the Pvs230 as a candidate molecule, and produced recombinant Pvs230 protein using cell-free system. Rabbit antisera against the rPvs230 reacted on the surface of P. vivax gametocytes taken from Thai patients. The antisera reduced the infectivity of P. vivax parasites to Anopheles dirus mosquitoes by membrane feeding assay.

Research Products

• [Journal Article] Plasmodium vivax gametocyte protein Pvs230 is a novel transmission-blocking vaccine candidate (2012)
  • Author(s): Tachibana M, Satoh C, Otsuki H, Sattabongkot J, Kaneko M, Torii M, Tsuboi T
  • Journal Title: Vaccine Volume: 30(10) Pages: 1807-1812
  • Peer Reviewed
• [Journal Article] Plasmodium vivax gametocyte protein Pvs230 is a novel transmission-blocking vaccine candidate (2012)
  • Author(s): Tachibana, et al.
  • Journal Title: Vaccine Volume: 30 Issue: 10 Pages: 1807-1812
  • DOI: 10.1016/j.vaccine.2012.01.003
  • Peer Reviewed
• [Journal Article] N-terminal pro-domain of Pfs230 synthesized using cell-free system is sufficient to induce the complement dependent malaria transmission-blocking activity (2011)
  • Author(s): Tachibana M, Wu Y, Iriko H, Muratova O, MacDonald NJ, Sattabongkot J, Takeo S, Otsuki H, Torii M, Tsuboi T
  • Journal Title: Clin Vaccine Immunol Volume: 18(8) Pages: 1343-1350
  • Peer Reviewed
• [Journal Article] N-terminal pro-domain of Pfs230 synthesized using cell-free system is sufficient to induce the complement dependent malaria transmission-blocking activity (2011)
  • Author(s): Tachibana, et al
  • Journal Title: Clin Vaccine Immunol Volume: 18 Issue: 8 Pages: 1343-1350
  • DOI: 10.1128/cvi.05104-11
  • Peer Reviewed
• [Presentation] ネズミマラリア原虫の蚊人工吸血法(メンブレンフィーディング法)の確立 (2012)
  • Author(s): 橘真由美、石野智子、横内ゆき、坪井敬文、鳥居本美
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 西宮市
• [Presentation] 三日熱マラリア伝搬阻止ワクチン候補抗原(Pvs25)の高分子量化による可溶性凝集体構築とそのワクチン効果 (2012)
  • Author(s): 宮田健、原國哲也、坪井敬文、Sattabongkot Jetsumon、橘真由美、鳥居本美、新川武
  • Organizer: 第81回日本寄生虫学会
  • Place of Presentation: 西宮市
• [Presentation] Identification of novel molecule that is specifically expressed on the surface of microgamete (2012)
  • Author(s): Tachibana M, Sudo M, Yokouchi Y, Ishino T, Tsuboi T, Torii M
  • Organizer: MAM 2012 Conference(Molecular Approaches to Malaria)
  • Place of Presentation: Lorne, Victoria, Australia
• [Presentation] 雄性生殖体に発現する新規伝搬阻止ワクチン候補抗原の同定 (2011)
  • Author(s): 橘真由美、須藤萠、横内ゆき、石野智子、坪井敬文、鳥居本美
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 東京都
• [Presentation] マラリア原虫スオオキネート表面に発現する新規タンパク質の同定と性状解析 (2011)
  • Author(s): Kangwanrangsan Niwat、橘真由美、坪井敬文、石野智子、鳥居本美
  • Organizer: 第80回日本寄生虫学会
  • Place of Presentation: 東京都
• [Presentation] A novel protein is targeted to the crystalloids of Plasmodium yoelii ookinetes (2010)
  • Author(s): Rachaneeporn Jenwithisuk、Niwat Kangwanrangsan、橘真由美、石野智子、坪井敬文、鳥居本美
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 北海道
• [Presentation] コムギ胚芽無細胞タンパク質合成法を用いた三日熱マラリア伝搬阻止ワクチン候補抗原Pvs230の作製 (2010)
  • Author(s): 橘真由美、石野智子、Rachaneeporn Jenwithisuk、Niwat Kangwanrangsan、横内ゆき、Statabongkot Jetsumon、坪井敬文、鳥居本美
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 北海道
• [Presentation] Immunization with N-terninal region of a gametocyte protein Pfs230 successfully induce transmissionblocking antibodies against Plasmodium falciparum (2009)
  • Author(s): Mayumi Tachibana, Hideyuki Iriko, Olga Muratova, Guanhong Song, Yimin Wu, Jetsumon Sattabongkot, Satoru Takeo, Hitoshi Otsuki, Motomi Torii, Takafumi Tsuboi
  • Organizer: American Society of Tropical Medicine and Hygiene 58^<th> Annual meeting
  • Place of Presentation: Washington, DC, USA
• [Remarks]
  • URL: http://www.m.ehime-u.ac.jp/school/parasitology/