Effects of betel quid chewing on the DNA methylation status of oral mucosa in south Asia

• Project/Area Number: 21406029
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Surgical dentistry
• Research Institution: Gifu University
• Principal Investigator: SHIBATA Toshiyuki 岐阜大学, 医学(系)研究科(研究院), 教授 (50226172)
• Co-Investigator(Kenkyū-buntansha): HARA Akira 岐阜大学, 大学院・医学系研究科, 教授 (10242728) ; KATO Keizou 岐阜大学, 医学部附属病院, 講師 (40397336) ; MAKITA Hiroki 岐阜大学, 大学院・医学系研究科, 講師 (50345790) ; 宮崎 康雄 岐阜大学, 医学部附属病院, 医員 (60509818) ; 山下 知巳 岐阜大学, 医学部附属病院, 准教授 (80345793)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥15,730,000 (Direct Cost: ¥12,100,000、Indirect Cost: ¥3,630,000); Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 口腔 / 粘膜病変 / メチル化異常 / 東南アジア / ヒト / 口腔がん / 噛タバコ

Research Abstract

To reveal the effects of DNA methylation status on the oral cancer carcinogenesis, we carried out the analysis of lesions in in betel quid chewers. First of all, we evaluated the swab sample accuracy using swab and tissue samples in promoter region of p16 and MGMT so on. DNA methylation status was well correlated between swab and tissue samples. Then, we analyzed DNA methylation status of Taiwan swab samples using pyrosequencing method focused on p16 and MGMT. 10% samples indicated positive. Furthermore, we examined the methylation positive of p16 in cancer patients and detected it in high ratio (almost 100%). These results possibly suggested the usefulness of p16 analysis as a biomarker in oral cancer.

Research Products

• [Journal Article] Effects of smoking and alcohol consumption on 5-fluorouracil‑related metabolic enzymes in oral squamous cell carcinoma (2014)
  • Author(s): T. Yashita, K. Kato, N .K. Long, H. Makita,K.Yonemoto,K.Iida,N.Tamaoki,D.Hatakeyama,T.Shibata
  • Journal Title: MOLECULAR AND CLINICAL ONCOLOGY Volume: Accepted February 18, 2014 Issue: 3 Pages: 429-434
  • DOI: 10.3892/mco.2014.267
  • Peer Reviewed
• [Journal Article] Necrotic cervical nodes : Usefulness of diffusion-weighted MR imaging in the differentiation of suppurative lymphadenitis from malignancy (2013)
  • Author(s): Kato H, Kanematsu M, Kato Z, Teramoto T, Mizuta, K, Aoki M, Makita H, Kato K
  • Journal Title: Eur J Radiol Volume: 82(1) Issue: 1 Pages: e28-e35
  • DOI: 10.1016/j.ejrad.2012.08.014
  • Peer Reviewed
• [Journal Article] Periostin directly and indirectly promotes tumor lymphangiogenesis of head and neck cancer (2012)
  • Author(s): Kudo Y, Iizuka S, Yoshida M, Nguyen PT, Siriwardena SB, Tsunematsu T, Ohbayashi M, Ando T, Hatakeyama D, Shibata T, Koizumi K, Maeda M, Ishimaru N, Ogawa I, Takata T
  • Journal Title: PLoS One Volume: 7(8) Issue: 8 Pages: e44488-e44488
  • DOI: 10.1371/journal.pone.0044488
  • NAID: 120006714446
  • Peer Reviewed
• [Journal Article] Periostin directly and indirectly promotes tumor lymphangiogenesis of head and neck cancer. (2012)
  • Author(s): Kudo Y, Iizuka S, Yoshida M, Nguyen PT, Siriwardena SB, Tsunematsu T, Ohbayashi M, Ando T, Hatakeyama D, Shibata T, Koizumi K, Maeda M, Ishimaru N, Ogawa I, Takata T
  • Journal Title: PLoS One. 2012;7(8):e44488. Epub 2012 Aug 30. Volume: 8
  • NAID: 120006714446
  • Peer Reviewed
• [Journal Article] Salivary gland function evaluated by diffusion-weighted MR imaging with gustatory stimulation : preliminary results (2011)
  • Author(s): Kato H, Kanematsu M, Toida M, Kawaguchi T, Shibata T, Kajita K, Hoshi H
  • Journal Title: Journal of Magnetic Resonance Imaging Volume: 34 Issue: 4 Pages: 909-909
  • DOI: 10.1002/jmri.22729
  • Peer Reviewed
• [Journal Article] A more efficient method to generate integration-free human iPS cells (2011)
  • Author(s): Okita, K., et al
  • Journal Title: Nature Methods Volume: 8 Issue: 5 Pages: 409-412
  • DOI: 10.1038/nmeth.1591
  • NAID: 120002949763
  • Peer Reviewed
• [Journal Article] Oral complaints and stimulated salivary flow rate in 1188 adults (2010)
  • Author(s): Toida M, Nanya Y, Takeda-Kawaguchi T, Baba S, Iida K, Kato K, Hatakeyama D, Makita H, Yamashita T, Shibata T
  • Journal Title: J Oral Pathol Med Volume: 39(5) Issue: 5 Pages: 407-19
  • DOI: 10.1111/j.1600-0714.2009.00852.x
  • Peer Reviewed
• [Journal Article] Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells (2010)
  • Author(s): Iida K, Takeda-Kawaguchi T, Tezuka Y, Kunisada T, Shibata T, Tezuka K
  • Journal Title: Arch Oral Biol Volume: 55(9) Pages: 648-54
  • Peer Reviewed
• [Journal Article] Dental pulp cells for induced pluripotent stem cell banking (2010)
  • Author(s): Tamaoki N, Takahashi K, Tanaka T, Ichisaka T, Aoki H, Takeda-Kawaguchi T, Iida K, Kunisada T, Shibata T, Yamanaka S, Tezuka K
  • Journal Title: J Dent Res Volume: 89(8) Issue: 8 Pages: 773-8
  • DOI: 10.1177/0022034510366846
  • Peer Reviewed
• [Journal Article] Suppressive effect of global DNA hypomethylation on gastric carcinogenesis (2010)
  • Author(s): Tomita H, Hirata A, Yamada Y, Hata K, Oyama T, Mori H, Yamashita S, Ushijima T, Hara A
  • Journal Title: Carcinogenesis Volume: 31(9) Issue: 9 Pages: 1627-33
  • DOI: 10.1093/carcin/bgq129
  • Peer Reviewed
• [Journal Article] Suppressive effect of global DNA hypomethylation on gastric carcinogenesis (2010)
  • Author(s): Tomita H, et al-7
  • Journal Title: Carcinogenesis Volume: 31 Pages: 1627-1633
  • Peer Reviewed
• [Journal Article] Global DNA hypomethylation suppresses squamous carcinogenesis in the tongue and esophagus (2009)
  • Author(s): Baba S, Yamada Y, Hatano Y, Miyazaki Y, Mori H, Shibata T, Hara A
  • Journal Title: Cancer Sci Volume: 100(7) Issue: 7 Pages: 1186-91
  • DOI: 10.1111/j.1349-7006.2009.01171.x
  • NAID: 10027191797
  • Peer Reviewed
• [Journal Article] The effect of neoadjuvant therapy on the 5-fluorouracil metabolic and relative enzymes of oral squamous cell carcinoma (2009)
  • Author(s): Yamashita T, Toida M, Kato K, Long NK, Miyazaki Y, Asaka Y, Hatakeyama D, Yonemoto K, Makita H, Kato Y, Shibata T
  • Journal Title: Oncol Rep Volume: 22(3) Pages: 501-7
  • NAID: 120006340795
  • Peer Reviewed
• [Journal Article] Susceptibilities of 23 Desulfovibrio isolates from humans (2009)
  • Author(s): Nakao K, Tanaka K, Ichiishi S, Mikamo H, Shibata T, Watanabe K.
  • Journal Title: Antimicrob Agents Chemother Volume: 53(12) Pages: 5308-11
  • Peer Reviewed
• [Journal Article] Aberrant promoter hypermethylation of the CHFR gene in oral squamous cell carcinomas (2009)
  • Author(s): Baba S, Hara A, Kato K, Long NK, Hatano Y, Kimura M, Okano Y, Yamada Y, Shibata T
  • Journal Title: Oncol Rep Volume: 22(5) Pages: 1173-9
  • NAID: 120006340800
  • Peer Reviewed
• [Presentation] Swabサンプルによる口腔がんのDNAメチル化異常の検討 (2013)
  • Author(s): 加藤恵三、吉田浩明、玉置也剛、飯田一規、米本和弘、畠山大二郎、牧田浩樹、柴田敏之
  • Organizer: 第58回日本口腔外科学会総会
  • Place of Presentation: 福岡
• [Presentation] ヒト口腔扁平上皮がんにおけるDNA メチル化異常の検討-擦過 標本と組織標本の相関性- (2012)
  • Author(s): 加藤 恵三, 玉置 也剛, 飯田 一規, 米本 和弘, 畠山 大二郎, 牧田 浩樹, 山下 知巳, 柴田 敏之
  • Organizer: 第19回日本がん予防学会
  • Place of Presentation: じゅうろくプラザ(岐阜市)
  • Year and Date: 2012-06-22
• [Presentation] Hypermethylation status of oral squamous cell carcinoma (2010)
  • Author(s): Shibata T
  • Organizer: Kaohsiung International Cancer Symposium
  • Place of Presentation: 台湾・高雄市
  • Year and Date: 2010-11-20
• [Presentation] Hypermethylation status of oral squamous cell carcinoma (2010)
  • Author(s): Shibata T
  • Organizer: Kaohsiung International Cancer Symposium
  • Place of Presentation: 高雄医科大学(台湾)
  • Year and Date: 2010-11-20
• [Presentation] 4NQO誘発ラット舌発癌モデルにおける遺伝子変異機構の解析 (2010)
  • Author(s): 牧田浩樹, 加藤恵三, 柴田敏之
  • Organizer: 第69回日本がん学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Hypermethylation of the RECK gene predicts poor prognosis in oral squamous cell carcinomas (2010)
  • Author(s): Nguyen Khanh Long, Keizo Kato, Tomomi Yamashita, Hiroki Makita, Makoto Toida, Daijiro Hatakeyama, Akira Hara, Hideki Mori, Toshiyuki Shibata
  • Organizer: 9th International conference of the Asian clinical oncology
  • Place of Presentation: Gifu
  • Year and Date: 2010-08-27
• [Presentation] ヒト口腔扁平上皮がんにおける DNA メチル化異常の検討─擦過 標本と組織標本の相関性─
  • Author(s): 加藤 恵三、玉置 也剛、飯田 一規、米本 和弘、畠山 大二郎、 牧田 浩樹、山下 知巳、柴田 敏之
  • Organizer: 第19回日本がん予防学会： 2012年6月22日(金)～23日(土)
  • Place of Presentation: じゅうろくプラザ（岐阜市）