Statistical Pattern Recognition in Small Sample Size Situations and its Application to Detection of Hepatocellular Carcinoma

• Project/Area Number: 21500173
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Perception information processing/Intelligent robotics
• Research Institution: Yamaguchi University
• Principal Investigator: HAMAMOTO Yoshihiko 山口大学, 大学院・医学系研究科, 教授 (90198820)
• Co-Investigator(Kenkyū-buntansha): IIZUKA Norio 山口大学, 医学部付属病院, 准教授 (80332807) ; FUJITA Yusuke 山口大学, 大学院・医学系研究科, 助教 (40509527)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 癌 / パターン認識 / 診断

Research Abstract

The purpose of this study is to develop a novel blood test system for detecting hepatocellular carcinoma(HCC) associated with hepatitics C virus(HCV) infection. In order to develop the novel test system, we must establish a feature selection method to find effective bio-makers in small training sample size situations, compared with very high dimensions. We found 2 methylated genes(SPINT2 and SRD5A2). With use of 2 methylated genes, AFP. and PIVKA II. we designed the blood test system for detecting HCC, which achieved high abilities(73. 2% sensitivity and 87. 7% specificity) for 258 test samples provided from 4 institutes. This suggests that our blood test system might lead to clinical applications.

Research Products

• [Journal Article] Efficient detection of hepatocellular carcinoma by a hybrido blood test of epigenetic and classical protein markers (2011)
  • Author(s): N. Iizuka, M. Oka, I. Sakaida, T. Moribe, T. Miura, N. Kimura, S. Tamatsukuri, H. Ishitsuka, K. Uchida, S. Terai, S. Yamashita, K. Okita, K. Sakata, Y. Karino, J. Toyota, E. Ando, T. Ide, M. Sata, R. Tsunedomi, M. Tsutsui, M. Iida, Y. Tokuhisa, K. Sakamoto, T. Tamesa, Y. Fujita, Y. Hamamoto
  • Journal Title: Clinica Chimica Acta Volume: 412 Pages: 152-158
  • Peer Reviewed
• [Journal Article] Efficient detection of hepatocellular carcinoma by a hybrid blood test of epigenetic and classical protein markers (2011)
  • Author(s): N.Iizuka, et al.
  • Journal Title: Clinica Chimica Acta Volume: 412 Pages: 152-158
  • Peer Reviewed
• [Journal Article] Methylated cyclin D2 gene circulating in the blood as a prognosis predictor of hepatocellular carcinoma (2010)
  • Author(s): M. Tsutsui, N. Iizuka, T. Moribe, T. Miura, N. Kimura, S. Tamatsukuri, H. Ishitsuka, Y. Fujita, Y. Hamamoto, R. Tsunedomi, M. Iida, Y. Tokuhisa, K. Sakamoto, T. Tamesa, I. Sakaida and M. Oka
  • Journal Title: Clinica Chimica Acta Volume: 411 Pages: 516-520
  • Peer Reviewed
• [Journal Article] Methylated cyclin D2 gene circulating in the blood as a prognosis predictor of hepatocellular carcinoma (2010)
  • Author(s): M.Tsutsui, et al.
  • Journal Title: Clinical Chimica Acta 411 Pages: 516-520
  • Peer Reviewed
• [Journal Article] Rapid and quantitative detection of CpG-methylationstatus using TaqMan PCR combined with methyl-binding-domain polypeptide (2009)
  • Author(s): N. Kimura, T. Moribe, N. Iizuka, T. Miura, S. Tamatsukuri, H. Ishitsuka, Y. Hamamoto and M. Oka
  • Journal Title: Clinical Biochemistry Volume: 42 Pages: 1113-1122
  • Peer Reviewed
• [Journal Article] Methylation of multiple genes as molecular markers for diagnosis of a small well-differentiated hepatocellular carcinoma (2009)
  • Author(s): T. Moribe, N. Iizuka, T. Miura, N. Kimura, S. Tamatsukuri, H. Ishitsuka, Y. Hamamoto, K. Sakamoto, T. Tamesa and M. Oka
  • Journal Title: Int. J. Cancer Volume: 125 Pages: 388-397
  • Peer Reviewed
• [Journal Article] Methylation of multiple genes as molecular markers for diagnosis of a small well-differentiated hepatocellular carcinoma (2009)
  • Author(s): T.Moribe, et al.
  • Journal Title: Int.J.Cancer 125 Pages: 388-397
  • Peer Reviewed
• [Journal Article] Rapid and quantitative detection of CpG-methylationstatus using TaqMan PCR combined with methyl-binding-domain polypeptide (2009)
  • Author(s): N.Kimura, et al
  • Journal Title: Clinical Biochemistry 42 Pages: 1113-1122
  • Peer Reviewed
• [Presentation] 血清CIMPと肝細胞癌の予後 (2011)
  • Author(s): 飯塚徳男、岡正朗、恒富亮一、藤田悠介、浜本義彦、森部豊輝
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場、名古屋
  • Year and Date: 2011-10-03
• [Presentation] 血清CIMPと肝細胞癌の予後 (2011)
  • Author(s): 飯塚, 他
  • Organizer: 第70回日本癌学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] An optimized Fisher linear classifier for detection of hepatocellular carcinoma, 8^<th> AACR-JCA Joint Conference Cancer Genomics (2010)
  • Author(s): Y. Fujita, Y. Hamamoto, N. Iizuka, M. Oka, T. Moribe
  • Organizer: Epigenomics and the Development of Novel Therapeutics
  • Place of Presentation: Hilton Waikoloa Village, Hawaii(米国)
  • Year and Date: 2010-02-06
• [Presentation] An optimized Fisher liner classifier for detection of hepatocellular car cinoma (HCC) (2010)
  • Author(s): Y.Fujita
  • Organizer: 8th AACR-JCA Joint Conference : Cancer Genomics, Epigenomies.and the Development of Novel Therapeutics
  • Place of Presentation: Hilton Waikoloa Village, Hawaii (米国)
  • Year and Date: 2010-02-06
• [Book] 統計的パターン認識入門 (2009)
  • Author(s): 浜本義彦
  • Total Pages: 122
  • Publisher: 森北出版
• [Remarks]
  • URL: http://www.ir.csse.yamaguchi-u.ac.jp/
• [Remarks] URL
  • URL: http://www.ir.csse.yamaguchi-u.ac.jp
• [Remarks]
  • URL: http://www.ir.csse.yamaguchi-u.ac.jp
• [Patent(Industrial Property Rights)] AFPおよびPIVKA-IIの測定値を特徴値とした識別関数を利用する、肝臓癌および慢性肝疾患の病態進行度の検出方法 (2009)
  • Inventor(s): 木村直紀、森部豊輝、玉造滋、飯塚徳男、岡正朗、浜本義彦
  • Industrial Property Rights Holder: エフホフマンーラロシュアーゲー
  • Industrial Property Number: 2009-094252
  • Filing Date: 2009-04-08
• [Patent(Industrial Property Rights)] 特許 (2005)
  • Inventor(s): 浜本義彦, 他
  • Industrial Property Rights Holder: 山口大学
  • Filing Date: 2005-12-02
  • Acquisition Date: 2011-04-08