Establishment of a method to measure absolute expression levels of all exons using microarrays
| Project/Area Number |
21500292
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | National Institute of Health Sciences |
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Principal Investigator |
AISAKI Ken-ichi 国立医薬品食品衛生研究所, 毒性部, 第一室長 (40322086)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイオインフォマティクス / ゲノミクス / アルゴリズム / 生体生命情報学 / マイクロアレイ |
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| Research Abstract |
Including exon-microarray, current microarray technology can detect only relative changes of mRNA expression. We tried to develop a new method to measure amount of each exon expression as copy number per one cell, for enhancing the performance study of splicing-variant in various situations. In the course of the study, we have revealed that serious errors are caused on exon microarray by "semi-specific hybridization", a kind of cross-hybridization among nucleotides sharing partially common sequence. As a result, we generated a new basic theory which can improve quality of exon-microarray data based on the sequence data of probes and targets taking the semi-specific hybridization into account.
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Report
(4 results)
Research Products
(8 results)
