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Molecular basis of neuron-specific, activity-dependent regulation of the plasticity-related gene Arc

Research Project

Project/Area Number 21500301
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe University of Tokyo

Principal Investigator

OKUNO Hiroyuki  東京大学, 大学院・医学系研究科, 助教 (80272417)

Co-Investigator(Renkei-kenkyūsha) BITO Haruhiko  東京大学, 大学院・医学系研究科, 准教授 (00291964)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsシナプス / 脳・神経 / ゲノム / 転写調節 / Arc / シグナル伝達 / 遺伝子 / 長期記憶 / 海馬
Research Abstract

The neuronal immediate-early gene Arc, which plays critical roles in long-term synaptic plasticity and long-term memory formation, is dynamically regulated by neuronal activities in the brain. Through this study, we have determined several genomic regulatory elements in the Arc promoter, and have identified transcription factors that contribute to activity-dependent expression. We further uncovered a novel mechanism by which Arc protein is localized and thus regulates glutamate receptor trafficking at synapses.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (36 results)

All 2012 2011 2010 2009 Other

All Journal Article (13 results) (of which Peer Reviewed: 10 results) Presentation (18 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Inverse synaptic tagging of inactive synapses via dynamic interaction of Arc/Arg3.1 with CaMKllβ2012

    • Author(s)
      奥野浩行, 他
    • Journal Title

      Cell

      Volume: 149 Issue: 4 Pages: 886-898

    • DOI

      10.1016/j.cell.2012.02.062

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Schema-dependent gene activation and memory encoding in neocortex2011

    • Author(s)
      Tse, D ; Takeuchi, T ; Kakeyama, M ; Kajii, Y ; Okuno, H ; Tohyama, C ; Bito, H, Morris, RGM.
    • Journal Title

      Science

      Volume: 333 Issue: 6044 Pages: 891-895

    • DOI

      10.1126/science.1205274

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Real-time measurements of protein dynamics using fluorescence activation-coupled protein labeling method2011

    • Author(s)
      Komatsu T, Johnsson K, Okuno H, Bito H, Inoue T, Nagano T, Urano Y
    • Journal Title

      J. Am. Chem. Soc

      Volume: 133 Issue: 17 Pages: 6745-6751

    • DOI

      10.1021/ja200225m

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation and function of immediate-early genes in the brain : beyond neuronal activity markers2010

    • Author(s)
      Okuno, H.
    • Journal Title

      Neuroscience Res

      Volume: 69 Issue: 3 Pages: 175-186

    • DOI

      10.1016/j.neures.2010.12.007

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synaptic Activity Responsive Element(SARE): a unique genomic structure with an unusual sensitivity to neuronal activity2010

    • Author(s)
      Inoue, M ; Yagishita-Kyo, N ; Nonaka, M ; Kawashima, T ; Okuno, H ; Bito, H.
    • Journal Title

      Commun. Integr. Biol

      Volume: 3 Issue: 5 Pages: 443-446

    • DOI

      10.4161/cib.3.5.12287

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synaptic tagging and capture : differential role of distinct calcium-calmodulin kinases in protein synthesis-dependent long-term potentiation2010

    • Author(s)
      Redondo, R. L ; Okuno, H ; Spooner, P. A ; Frenguelli, B. G ; Bito, H ; Morris, R. G. M.
    • Journal Title

      J. Neurosci.

      Volume: 30 Issue: 14 Pages: 4981-4989

    • DOI

      10.1523/jneurosci.3140-09.2010

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] シナプスから核へのシグナリングとシナプス活動依存的遺伝子発現:前初期遺伝子Arcの発現制御メカニズムを中心に2010

    • Author(s)
      奥野浩行
    • Journal Title

      生化学

      Volume: 82 Pages: 841-846

    • NAID

      10027502590

    • URL

      http://wwwsoc.nii.ac.jp/jbiochem/magazine/82-09-07.pdf

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation and function of immediate-early genes in the brain : be yond neuronal activity markers.2010

    • Author(s)
      Okuno H.
    • Journal Title

      Neuroscience Res.

      Volume: 69 Pages: 175-186

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synaptic Activity Responsive Element (SARE): a unique genomic structure with an unusual sensitivity to neuronal activity.2010

    • Author(s)
      Inoue M, Yagishita-Kyo N, Nonaka M, Kawashima T, Okuno H, Bito H.
    • Journal Title

      Commun.Integr.Biol.

      Volume: 3 Pages: 443-446

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Synaptic tagging and capture : differential role of distinct calcium/calmodulin kinases in protein synthesis-dependent long-term potentiation.2010

    • Author(s)
      Redondo RL, Okuno H, Spooner PA, Frenguelli BCC Bito H, Morris RGM.
    • Journal Title

      J.Neurosci.

      Volume: 30 Pages: 4981-4989

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synaptic tagging and capture : differential role of distinct calcium-calmodulin kinases in protein synthesis-dependent long-term potentiation.2010

    • Author(s)
      Redondo, R.L., Okuno, H., Spooner, P.A., Frenguelli, B.G., Bito, H., Morris, R.G.M
    • Journal Title

      Journal of Neuroscience 30

      Pages: 4981-4989

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シナプスから核へのシグナリング:シナプス可塑性を長期化する分子機構2009

    • Author(s)
      奥野浩行、川島尚之、野中美応、尾藤晴彦
    • Journal Title

      細胞工学

      Volume: 28 Pages: 894-899

    • Related Report
      2011 Final Research Report
  • [Journal Article] シナプスから核へのシグナリング:シナプス可塑性を長期化する分子機構2009

    • Author(s)
      奥野浩行、川島尚之、野中美応、尾藤晴彦
    • Journal Title

      細胞工学 28

      Pages: 894-899

    • Related Report
      2009 Annual Research Report
  • [Presentation] Enhanced synaptic accumulation of activity-driven Arc protein during sensory deprivation in the mouse visual cortex2011

    • Author(s)
      Okuno, H ; Fujii, H ; Kawashima, T ; Nonaka, M ; Yagishita-Kyo, N ; Ishii, Y ; Takemoto-Kimura, S ; Chowdhury, S ; Worley, PF ; Bito, H.
    • Organizer
      第40回北米神経科学学会年会(Neuroscience 2010)
    • Place of Presentation
      San Diegoコンベンションセンター(米国)
    • Year and Date
      2011-11-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Real-time imaging of synaptic receptor trafficking using a fluorescence activation-coupled protein labeling(FAPL) method2011

    • Author(s)
      Ishi, Y ; Okuno, H ; Tomatsu, T ; Nagano, T ; Urano, Y ; Bito, H.
    • Organizer
      第41回北米神経科学学会年会(Neuroscience 2011)
    • Place of Presentation
      Washington DCコンベンションセンター(米国)
    • Year and Date
      2011-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Increased nuclear shuttling of CRTC1 enhances activity-regulated CREB-dependent gene expression and long-term memory formation2011

    • Author(s)
      Nonaka M ; Fukushima, H ; Kawashima, T ; Suzuki, K ; Okuno, H ; Kida, S ; Bito, H
    • Organizer
      第41回北米神経科学学会年会(Neuroscience 2011)
    • Place of Presentation
      Washington DCコンベンションセンター(米国)
    • Year and Date
      2011-11-15
    • Related Report
      2011 Final Research Report
  • [Presentation] Gene activation and memory encoding in neocortex during hippocampus-dependent learning2011

    • Author(s)
      Morris, RG.; Tse, D ; Takeuchi, T ; Kakeyama, M ; Kajii, Y ; Okuno, H ; Toyama, C ; Bito, H.
    • Organizer
      第41回北米神経科学学会年会(Neuroscience 2011)
    • Place of Presentation
      Washington DCコンベンションセンター(米国)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanisms of Arc targeting to synapses2011

    • Author(s)
      Okuno, H.
    • Organizer
      The 6th International Conference of Neurons and Brain Diseases/Association for the Study of Neurons and Diseases
    • Place of Presentation
      富山クラウンプラザホテル(富山県)
    • Year and Date
      2011-08-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Mechanisms of Arc targeting to synapses2011

    • Author(s)
      Okuno, H.
    • Organizer
      The 6th International Conference of Neurons and Brain Diseases/Association for the Study of Neurons and Diseases
    • Place of Presentation
      富山クラウンプラザホテル (富山県富山市)(招待講演)
    • Year and Date
      2011-08-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enhanced synaptic accumulation of activity-driven Arc protein during sensory deprivation in the mouse visual cortex.2010

    • Author(s)
      Okuno H, Fujii H, Kawashima T, Nonaka M, Yagishita-Kyo N, Ishii Y, Takemoto-Kimura S, Chowdhury S, Worley PF, Bito H.
    • Organizer
      北米神経科学学会年会(Neuroscience 2010)
    • Place of Presentation
      San Diegoコンベンションセンター(米国)
    • Year and Date
      2010-11-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dual FRET-based analysis of biochemical computation performed by neuronal Ca2+signaling processes2010

    • Author(s)
      Fujii, H ; Inoue, M ; Okuno, H ; Takemoto-Kimura, S ; Bito, H
    • Organizer
      第40回北米神経科学学会年会(Neuroscience 2010)
    • Place of Presentation
      San Diegoコンベンションセンター(米国)
    • Year and Date
      2010-11-16
    • Related Report
      2011 Final Research Report
  • [Presentation] Synaptic inactivity enhances postsynaptic contents of activity-induced Arc protein in the neocortex2010

    • Author(s)
      Okuno, H ; Kawashima, T ; Yagishita-Kyo, N ; Ishii, Y ; Takemoto-Kimura, S ; Bito, H.
    • Organizer
      第33回日本神経科学学会年会(Neuro2010)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Year and Date
      2010-09-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Synaptic inactivity enhances postsynaptic contents of activity-induced Arc protein in the neocortex.2010

    • Author(s)
      Okuno H, Kawashima T, Yagishita-Kyo N, Ishii Y, Takemoto-Kimura S, Bito H.
    • Organizer
      第33回日本神経科学学会年会(Neuro2010)
    • Place of Presentation
      神戸コンベンションセンター(兵庫県神戸市)
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multiple regulatory elements in the Arc/Arg3. 1 promoter essential for synaptic activity-responsive gene expression in activated neurons2009

    • Author(s)
      Kawashima, K ; Okuno, H ; Bito, H.
    • Organizer
      第32回日本分子生物学会年会(MBSJ2009)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2009-12-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Multiple regulatory elements in the Arc/Arg3.1 promoter essential for synaptic activity-responsive gene expression in activated neurons.2009

    • Author(s)
      Kawashima, K., Okuno, H., Bito, H.
    • Organizer
      第32回日本分子生物学会年会(MBSJ2009)
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-12-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Activity-dependent gene regulation and nucleus-to-synapse signaling for synaptic plasticity "UNAM Symposium"2009

    • Author(s)
      Okuno, H.
    • Organizer
      Learning and Memory
    • Place of Presentation
      UNAM神経生物学研究所(ケラタロ州・メキシコ)
    • Year and Date
      2009-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Activity-dependent gene regulation and nucleus-to-synapse signaling for synaptic plasticity2009

    • Author(s)
      Okuno, H.
    • Organizer
      UNAM Symposium "Learning and Memory"
    • Place of Presentation
      UNAM神経生物学研究所(ケラタロ州・メキシコ)
    • Year and Date
      2009-11-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Differential role of distinct calcium-calmodulin kinases in protein synthesis-dependent long-term potentiation2009

    • Author(s)
      Redondo, R. L ; Okuno, H ; Spooner, P. A ; Frenguelli, B. G ; Bito, H ; Morris, RG. M.
    • Organizer
      第39回北米神経科学学会年会(Neuroscience 2009)
    • Place of Presentation
      Chicagoコンベンションセンター(米国)
    • Year and Date
      2009-10-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Single spine dual FRET imaging to better understanding synaptic biochemical network2009

    • Author(s)
      Fujii, H ; Inoue, M ; Ishii, Y ; Okuno, H ; Bito, H.
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋国際会議場(愛知県)
    • Year and Date
      2009-09-18
    • Related Report
      2011 Final Research Report
  • [Presentation] Synaptic activity-dependent regulation of plasticity-related gene Arc/Arg3. 1.2009

    • Author(s)
      Okuno, H ; Kawashima, T ; Nonaka, M ; Nan, K ; Bito, H.
    • Organizer
      IUPS2009
    • Place of Presentation
      京都国際会議場(京都府)
    • Year and Date
      2009-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Synaptic activity-dependent regulation of plasticity-related gene Arc/Arg3.12009

    • Author(s)
      Okuno, H., Kawashima, T., Mio, N., Nan, K., Bito, H
    • Organizer
      IUPS2009
    • Place of Presentation
      京都国際会議場(京都)
    • Year and Date
      2009-07-28
    • Related Report
      2009 Annual Research Report
  • [Book] Encyclopedia of Signaling Molecules2012

    • Author(s)
      Yagishita-Kyo, N ; Inoue, M ; Nonaka, M ; Okuno, H ; Bito H.
    • Publisher
      Springer(印刷中)
    • Related Report
      2011 Final Research Report
  • [Book] "CREB" in "Encyclopedia of Signaling Molecules"2012

    • Author(s)
      Yagishita-Kyo N, Inoue M, Nonaka M, Okuno H, Bito H
    • Publisher
      Springer(印刷中)
    • Related Report
      2011 Annual Research Report
  • [Book] CREB in "Encyclopedia of Signaling Molecules"2011

    • Author(s)
      Yagishita-Kyo N, Inoue M, Nonaka M, Okuno H, Bito H.
    • Publisher
      Springer(掲載確定(印刷中))
    • Related Report
      2010 Annual Research Report
  • [Remarks] 本研究成果の一部は以下のURLで一般に公開されている。(1)東京大学Todai Research

    • URL

      http://www.u-tokyo.ac.jp/ja/todai-research/research-information/the-law-of-the-synapses-punishing-the-weak-to-maintain-strong-synapses-strong/

    • Related Report
      2011 Final Research Report
  • [Remarks] (2)科学技術振興機構プレスリリース

    • URL

      http://www.jst.go.jp/pr/announce/20120511/index.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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