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Subtype specification of the olfactory sensory neurons in Drosophila by RUNX family transcription factor Lozenge

Research Project

Project/Area Number 21500302
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionThe University of Tokyo

Principal Investigator

ENDO Keita  東京大学, 分子細胞生物学研究所, 助教 (40425616)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords神経クラス分化 / 嗅覚神経細胞 / Runx / Notch / エピジェネティック / 細胞運命決定 / Notchシグナル / Hamlet / クロマチン / 非対称分裂 / エピジェネティックス / Lozenge / 神経前駆細胞 / 経時的な発現量の変化 / ショウジョウバエ / 嗅覚 / 神経サブタイプ分化 / RUNX / EGFR
Research Abstract

Olfactory sensory neurons in Drosophila melanogaster are diversified into 50 different classes, each expressing specific odorant receptors, and together contributing to detection and discrimination of a myriad of odorous chemicals in environment. To reveal the developmental mechanisms that generate this neuronal diversity, I analyzed the function of two molecules ; Runx-family transcription factor Lozenge and Drosophila homologue of Evi1 proto-oncogene Hamlet. I found that the precursor cells for the olfactory sensory neurons differentiate continuously while Lozenge level gradually decreases during development. This makes precursor cells that are differentiated at different time points receive different levels of Lozenge, thereby generating different classes of olfactory sensory neurons. I also found that Hamlet contributes to the process of a single precursor cell generating different classes of olfactory sensory neurons, by modifying the expression of the Notch-target genes through epigenetic mechanisms.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2012 2011 2010 2009 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (21 results) Remarks (4 results)

  • [Journal Article] Chromatin modification of Notch targets in olfactory receptor neuron diversification2012

    • Author(s)
      Endo, K., Karim, M.R., Taniguchi, H., Krejci, A., Kinameri, E., Siebert, M., Ito, K., Bray, S.J., Moore, A.W.
    • Journal Title

      Nat Neurosci

      Volume: 15 Issue: 2 Pages: 224-233

    • DOI

      10.1038/nn.2998

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Presentation] キイロショウジョウバエにおける嗅覚神経細胞のクラス分化機構2011

    • Author(s)
      遠藤啓太
    • Organizer
      第179回つくばブレインサイエンス(TBSA)・セミナー
    • Place of Presentation
      筑波大学医学地区・臨床講堂
    • Year and Date
      2011-02-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Olfactory Receptor Neuronsare Diversified by the Prdm ProteinHamlet that Mediates ChromatinModification at Notch-Target Loci2011

    • Author(s)
      遠藤啓太
    • Organizer
      1st Asia-Pacific Drosophila Research Conference
    • Place of Presentation
      Taipei
    • Related Report
      2011 Final Research Report
  • [Presentation] Olfactory Receptor Neuron Identity is Diversified by theDrosophila Evi1/Prdm16 HomologueHamlet that Mediates ChromatinModification at Notch-Target Loci2011

    • Author(s)
      遠藤啓太
    • Organizer
      第44回発生生物学会大会
    • Place of Presentation
      沖縄
    • Related Report
      2011 Final Research Report
  • [Presentation] キイロショウジョウバエにおける嗅覚神経細胞のクラス分化機構2011

    • Author(s)
      遠藤啓太
    • Organizer
      第179回つくばブレインサイエンス(TBSA)・セミナー
    • Place of Presentation
      つくば
    • Related Report
      2011 Final Research Report
  • [Presentation] Olfactory Receptor Neuron Identity is Diversified by the Drosophila Evil/Prdm16 Homologue Hamlet that Mediates Chromatin Modification at Notch-Target Loci2011

    • Author(s)
      Keita Endo, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Hiroaki Taniguchi, Matthias Siebert, Kei Ito, Sarah J.Bray, Adrian W.Moore
    • Organizer
      第44回発生生物学会大会
    • Place of Presentation
      沖縄・沖縄コンベンションセンター
    • Related Report
      2011 Annual Research Report
  • [Presentation] Olfactory Receptor Neurons are Diversified by the Prdm Protein Hamlet that Mediates Chromatin Modification at Notch-Target Loci2011

    • Author(s)
      Keita Endo, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Hiroaki Taniguchi, Matthias Siebert, Kei Ito, Sarah J.Bray, Adrian W.Moore
    • Organizer
      1st Asia-Pacific Drosophila Research Conference
    • Place of Presentation
      台湾・台北・Chientan Youth Activity Center
    • Related Report
      2011 Annual Research Report
  • [Presentation] Resetting of Notch signalingby hamlet ensures the repeated use ofthe Notch-mediated cell-fatespecification at each cell division todiversify the Drosophila olfactorysensory neurons2010

    • Author(s)
      遠藤啓太
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] キイロショウジョウバエ嗅覚神経細胞の多様性は細胞系譜特異的なNotchシグナルの修飾により獲得される2010

    • Author(s)
      遠藤啓太
    • Organizer
      第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] Sub-lineage-Specific Modification of Notch Transcriptional Regulation by Hamlet Generates Drosophila Olfactory Sensory Neuron Diversity2010

    • Author(s)
      遠藤啓太
    • Organizer
      第43回発生生物学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Sub-lineage-Specific Modification of Notch Transcriptional Regulation by Hamlet Generates Drosophila Olfactory Sensory Neuron Diversity2010

    • Author(s)
      Keita Endo, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Matthias Seibert, Kei Ito, Sarah J.Bray, Adrian W.Moore
    • Organizer
      第43回発生生物学会大会
    • Place of Presentation
      京都・国立京都国際会館
    • Related Report
      2010 Annual Research Report
  • [Presentation] キイロショウジョウバエ嗅覚神経細胞の多様性は細胞系譜特異的なNotchシグナルの修飾により獲得される2010

    • Author(s)
      遠藤啓太, MD Rezaul Karim, Alena Krejci, 木滑恵未, Matthias Seibert, 伊藤啓, Sarah J.Bray, Adrian W.Moore
    • Organizer
      第33回日本神経科学大会・第53回日本神経化学会大会・第20回日本神経回路学会大会合同大会
    • Place of Presentation
      神戸・神戸コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Resetting of Notch signaling by hamlet ensures the repeated use of the Notch-mediated cell-fate specification at each cell division to diversify the Drosophila olfactory sensory neurons2010

    • Author(s)
      Keita Endo, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Matthias Seibert, Kei Ito, Sarah J.Bray, Adrian W.Moore
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸・神戸コンベンションセンター
    • Related Report
      2010 Annual Research Report
  • [Presentation] Combinatorial role of theEGFR signaling pathway and a RUNXfamily transcription factor Lozenge inthe subtype specification of the Drosophila olfactory sensory neurons2009

    • Author(s)
      遠藤啓太
    • Organizer
      第32回日本神経科学大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Final Research Report
  • [Presentation] Lineage-specific modificationof Notch signaling by Hamletgenerates Drosophila olfactory neurondiversity2009

    • Author(s)
      遠藤啓太
    • Organizer
      9th Japanese Drosophila Research Conference
    • Place of Presentation
      嬬恋
    • Related Report
      2011 Final Research Report
  • [Presentation] Combinatorial role of theEGFR signaling pathway and a RUNXfamily transcription factor Lozenge inthe subtype specification of the Drosophila olfactory sensory neurons2009

    • Author(s)
      遠藤啓太
    • Organizer
      9th Japanese Drosophila Research Conference
    • Place of Presentation
      嬬恋
    • Related Report
      2011 Final Research Report
  • [Presentation] Combinatorial role of the EGFR signaling pathway and a RUNX family transcription factor Lozenge in the subtype specification of the Drosophila olfactory sensory neurons2009

    • Author(s)
      Keita Endo, Shohei Kuwa, Kei Ito
    • Organizer
      The 9th Japanese Drosophila Research Conference
    • Place of Presentation
      掛川・ヤマハリゾートつま恋
    • Related Report
      2009 Annual Research Report
  • [Presentation] Important role of the EGFR signal pathway for the diversification of olfactory receptor neurons in Drosophila2009

    • Author(s)
      Shohei Kuwa, Keita Endo, Kei Ito
    • Organizer
      The 9th Japanese Drosophila Research Conference
    • Place of Presentation
      掛川・ヤマハリゾートつま恋
    • Related Report
      2009 Annual Research Report
  • [Presentation] Linage-specific modification of Notch signaling by Hamlet generates Drosophila olfactory neuron diversity2009

    • Author(s)
      Keita Endo, Emi Kinameri, Matthias Seibert, Andrew Liu, Kei Ito, Adrian Moore
    • Organizer
      The 9th Japanese Drosophila Research Conference
    • Place of Presentation
      掛川・ヤマハリゾートつま恋
    • Related Report
      2009 Annual Research Report
  • [Presentation] Combinatorial role of the EGFR signaling pathway and a RUNX family transcription factor Lozenge in the subtype specification of the Drosophila olfactory sensory neurons2009

    • Author(s)
      Keita Endo, Kei Ito
    • Organizer
      Neuroscience 2009(第32回日本神経科学大会)
    • Place of Presentation
      名古屋・名古屋国際会議場
    • Related Report
      2009 Annual Research Report
  • [Presentation] A Drosophila Runx family transcription factor, Lozenge, specifies the olfactory receptor neuron class in a dose-dependent manner2009

    • Author(s)
      Keita Endo, Shohei Kuwa, Kei Ito
    • Organizer
      CSHL meeting : Neurobiology of Drosophila
    • Place of Presentation
      米国・コールドスプリングハーバー
    • Related Report
      2009 Annual Research Report
  • [Presentation] A Drosophila RUNX familytranscription factor, Lozenge, specifiesthe olfactory receptor neuron class in adoes-dependent manner

    • Author(s)
      遠藤啓太
    • Organizer
      CSHLmeeting "Neurobiology of Drosophila"
    • Place of Presentation
      Cold Spring Harbor, New York
    • Related Report
      2011 Final Research Report
  • [Remarks] 新聞報道(1)日刊工業新聞「理研と東大、嗅覚神経細胞をつくる仕組みを解明-iPS細胞分化に貢献」2012年1月9日

    • Related Report
      2011 Final Research Report
  • [Remarks] (2)日経バイオテクONLINE「理研BSIと東大IMCB、嗅覚神経細胞分化でNotchとHamletがエピジェネティクス制御、12月24日に説明会」2011年12月28日

    • Related Report
      2011 Final Research Report
  • [Remarks] (3)化学工業日報「嗅覚神経細胞の多様な分化/エピジェネティクスな現象/核内因子が標的遺伝子制御」2011年12月26日

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.iam.u-tokyo.ac.jp/NatureNeuroscience,doi10.1038,nn.2998.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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