Effects of proteases on differentiation and maturation of oligodendrocytes

• Project/Area Number: 21500322
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Asahikawa Medical College
• Principal Investigator: YOSHIDA Shigetaka 旭川医科大学, 医学部, 教授 (20230740)
• Co-Investigator(Kenkyū-buntansha): BANDO Yoshio 旭川医科大学, 医学部, 講師 (20344575) ; MURAKAMI Koichi 旭川医科大学, 医学部, 助教 (90400085)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: オリゴデンドロサイト / プロテアーゼ / KLK6 / 発達 / ミエリン / 脱髄

Research Abstract

KLK6 is a serine protease specifically expressed in oligodendrocytes. The function of the protease on the maturation of oligodendrocyte has been undetermined. We analyzed KLK6 gene knockout (KLK6-KO) mice and found that the development of oligodendrocytes was temporally delayed. We also found that the expression of myelin basic protein was smaller in KLK6-KO mice than that of wild-type mice. These results indicate that KLK6 plays a role in the development of oligodendrocytes.

Research Products

• [Journal Article] Kallikrein-related peptidase 8-dependent skin wound healing is associated with upregulation of kallikrein-related peptidase 6 and PAR2 (2012)
  • Author(s): Kishibe M, Bando Y, Tanaka T, Ishida-Yamamoto A, Iizuka H, Yoshida S
  • Journal Title: J Invest Dermatol Volume: (印刷中) Issue: 6 Pages: 1717-1724
  • DOI: 10.1038/jid.2012.18
  • Peer Reviewed
• [Journal Article] Klk8, a Multifunctional Protease in the Brain and Skin : Analysis of Knockout Mice (2010)
  • Author(s): Yoshida S
  • Journal Title: Biol Chem Volume: 391 Pages: 375-380
  • Peer Reviewed
• [Journal Article] オリゴデンドロサイトの生死とプロテアーゼ (2010)
  • Author(s): 吉田成孝
  • Journal Title: 脳21 Volume: 13 Pages: 9-14
• [Journal Article] オリゴデンドロサイトの生死とプロテアーゼ (2010)
  • Author(s): 吉田成孝
  • Journal Title: 脳21 13 Pages: 9-14
• [Journal Article] Klk8, a Multifunctional Protease in the Brain and Skin : Analysis of Knockout Mice. (2010)
  • Author(s): Yoshida S
  • Journal Title: Biol.Chem. (印刷中)
  • Peer Reviewed
• [Presentation] 実験的自己免疫性脳脊髄炎モデルを用いた脱髄の形態学的解析 (2011)
  • Author(s): 野村太一、板東良雄、暮地本宙己、渡部剛、吉田成孝
  • Organizer: 日本解剖学会第57回東北・北海道連合支部学術集会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-09-11
• [Presentation] The delay of oligodendrocyte development and myelination in KLK6 deficient mouse (2011)
  • Author(s): Murakami K, Jiang Y-P, TanakaT, Bando Y, Yoshida S
  • Organizer: 4th International Symposium on Kallikrein-Related Peptidases (ISK2011)
  • Place of Presentation: Rhodes Island、Greece
  • Year and Date: 2011-09-02
• [Presentation] Morphological changes of axons and myelin in experimental autoimmune encephalomyelitis (2011)
  • Author(s): BandoY, Nomura T, Bochimoto H, Kouga D, Watanabe T, Yoshida S
  • Organizer: 10th Biennial ISN Satellite Meeting on Myelin Biology
  • Place of Presentation: Kolymvari, Crete, Greece
  • Year and Date: 2011-08-24
• [Presentation] KLK6ノックアウトマウスにおけるミエリン関連タンパクの発現変動 (2010)
  • Author(s): 村上公一、板東良雄、吉田成孝
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-03
• [Presentation] マウス多発性硬化症モデルにおけるKLK6を介した脱髄分子機所 (2010)
  • Author(s): 板東良雄、村上公一、吉田成孝
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-02
• [Presentation] KLK6ノックアウトマウスにおけるオリゴデンドロサイト関連マーカーの発現 (2009)
  • Author(s): 村上公一, 板東良雄, 吉田成孝
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] KLK8, a Multifunctional Protease in the Brain and Skin : Analysis of Knockout Mice (2009)
  • Author(s): Yoshida S, Shiosaka S
  • Organizer: 3rd International Symposium on Kallikreins and Kallikrein-related Peptidases
  • Place of Presentation: ミュンヘン
  • Year and Date: 2009-09-01