| Project/Area Number |
21500329
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TANAKA Masaki 京都府立医科大学, 医学研究科, 准教授 (80264753)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshihisa 京都府立医科大学, 医学研究科, 助教 (50363990)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | relaxin-3 / knock out / behavioral test / anxiety / mouse / food intake / CRF / alcohol / elevated plus maze / intracerebroventricular / c-Fos / ACTH / SATAT5A / gene expression / promoter analysis |
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| Research Abstract |
In order to investigate the function of newly peptide relaxin-3(RLN3), we have made gene knockout mice and examined a battery of the behavioral tests. RLN3 KO mice exhibited normal growth and appearance, and were generally indistinguishable from wild genotype littermates. There was no difference in bodyweight among genotypes. However, KO mice exhibited a robust increase in the tendency to enter open arms in the elevated plus maze test. Thus RLN3 KO mice exhibit mild anxiolytic characteristics relative to wild-type mice, suggesting that this peptide is involved in anxiety-related behavior(Watanabe Y et al, Front Behav Neurosci 2011). We also investigated the hypothalamic action of RLN3 to stress-responding system by administrating it into the cerebral ventricle. RLN3 induced larger number of c-Fos expressions in the paraventricular nucleus of the hypothalamus(PVN) after 1nmol icv injection compared with saline injection. A part of c-Fos in the anterior PVN was induced in the CRF containing neurons. Plasma ACTH level was also increased 15 min after RLN3 administration. These results suggest that RLN3 is able to stimulate hypothalamo-pituitary CRF-ACTH system which responds to external stress in acute phase(Watanabe Y et al, J Mol Neurosci 2011). I wrote a review article to summarize the function of RLN3 in the view of stress and food intake(Tanaka M, FEBS J, 2010).
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