| Project/Area Number |
21500333
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
INOUE Takayoshi 独立行政法人国立精神・神経医療研究センター, 神経研究所・疾病研究第六部, 室長 (20370984)
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| Co-Investigator(Renkei-kenkyūsha) |
INOUE Yukiko 国立精神・神経医療研究センター, 神経研究所・疾病研究第六部, 流動研究員 (30611777)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 神経実験形態学 / 脳・神経 / 遺伝子発現制御 / ゲノム / 細胞・組織 / 発生・分化 / トランスジェニックマウス / 細胞接着 |
|---|
| Research Abstract |
In the present research project, an original and efficient bacterial artificial chromosome-based enhancer bashing methodology was applied to clarify the detailed molecular machinery for mouse forebrain compartmentalization. As the results, a 110-bp fragment located approximately 40-kb upstream of the cell adhesion molecule Cdh6 gene transcription start site was confirmed to be necessary yet insufficient for the forebrain compartment specific expression. Importantly, together with the 110-bp fragment, an evolutionary conserved region that does not contain any forebrain specific master control gene Pax6 binding motifs was found to be synergistically sufficient for the Cdh6 expression, highlighting the value of combinatorial regulatory cascades in specifying the forebrain compartment as patterning basis for the elaborated cellular structure and function.
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