Biochemical and neuropathological analysis of HtrA2 knockout mouse
Project/Area Number |
21500336
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Nerve anatomy/Neuropathology
|
Research Institution | Kyoto University |
Principal Investigator |
ITO Hidefumi 京都大学, 医学研究科, 講師 (20250061)
|
Co-Investigator(Kenkyū-buntansha) |
TAKAHASHI Ryosuke 京都大学, 医学研究科, 教授 (90216771)
KAWAMOTO Yasuhiro 京都大学, 医学研究科, 非常勤講師 (40335253)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | パーキンソン病 / HtrA2 / knockout mouse / ノックアウトマウス / Parkinson' sdisease / Parkinson's disease / Parkinson' s disease |
Research Abstract |
We generated HtrA2 knockout(KO) mice, and analyzed them biochemically and neuropathologically. We found that the mean survival of the homozygous HtrA2 KO mice was 30 days. In the mice, the striatum was degenerated and ubiquitinated proteins were accumulated. In addition, we found reduction of COXI, COXIV, and parkin proteins, and increase of LC3-II. Their skeletal and cardiac muscles showed atrophy. The mean survival of HtrA2/parkin double KO mice was shorter than that of HtrA2 KO mice. Moreover, we identified HtrA2 immunoreactivity in the senile plaques and the neurofibrillary tangles of Alzheimer's and in the intracytoplasmic inclusions of ALS patients.
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] Cilostazol, a phosphodiesterase inhibitor, prevents no-reflow and hemorrhage in mice with focal cerebral ischemia2012
Author(s)
Hase Y, Okamoto Y, Fujita Y, KitamuraA, Ito H, Maki T, Washida K, TakahashiR, Ihara M
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Journal Title
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Volume: 233
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Pages: 523-533
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[Journal Article] Selective white matter abnormalities in a novel rat model of vascular dementia2012
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[Journal Article] Clinicopathologic study on an ALS family with a heterozygous E478G optineurin mutation2011
Author(s)
Ito H, Nakamura M, Komure O, Ayaki T, Wate R, Maruyama H, Nakamura Y, FujitaK, Kaneko S, Okamoto Y, Ihara M, Konishi T, Ogasawara K, Hirano A, Kusaka H, KajiR, Takahashi R, Kawakami H
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[Journal Article] Optineurin is co-localized with FUS in basophilic inclusions of ALS with FUS mutation and in basophilic inclusion body disease2011
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[Journal Article] The influence of chronic cerebral hypoperfusion on cognitive function and amyloid b metabolism in APP overexpressing mice2011
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[Journal Article] Mutations of optineurin in amyotrophic lateral sclerosis2010
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Maruyama H, Morino H, Ito H, Izumi Y, Kato H, Watanabe Y, Kinoshita Y, Kamada M, Nodera H, Suzuki H, Komure O, Matsuura S, Kobatake K, Morimoto N, Abe K, Suzuki N, Aoki M, Kawata A, Hirai T, Kato T, Ogasawara K, Hirano A, Takumi T, Kusaka H, Hagiwara K, K
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[Journal Article] A mouse model characterizing features of vascular dementia with hippocampal atrophy2010
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Stroke
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[Journal Article] Screening for TARDBP mutations in Japanese familial amyotrophic lateral sclerosis2009
Author(s)
Kamada M, Maruyama H, Tanaka E, Morino H, Wate R, Ito H, Kusaka H, Kawano Y, Miki T, Nodera H, Izumi Y, Kaji R, Kawakami H
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[Presentation] Optineurin in ALS2011
Author(s)
Kawakami H, Maruyama H, Ito H
Organizer
第52回日本神経学会学術大会AAN-JSN Joint Symposium
Place of Presentation
Tokyo
Year and Date
2011-05-18
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[Presentation] Clinical and Neuropathologic Investigation of a Family with ALS Associated with Mutated Optineurin2010
Author(s)
Ito H, Nakamura M, Komure O, Maruyama M, Wate R, Nakamura Y, Fujita K, Kaneko S, Ogasawara O, Nakabayashi H, Ayaki T, Okamoto Y, Ihara M, Kaji R, Kusaka H, Hirano A, Takahashi R, Kawakami H
Organizer
XVIIth International Congress of Neuropathology
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Salzburg, Austria
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[Presentation] A Mouse Model Characterizing Features of Vascular Dementia with Hippocampal Atrophy2010
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Ihara M, Nishio K, Kakaria RN, Maki T, Fujita Y, Ito H, Oishi N, Fukuyama H, Takahashi R, Tomimoto H
Organizer
XVIIth International Congress of Neuropathology
Place of Presentation
Salzburg, Austria
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