Analysis of the molecular mechanism of the repair of demyelination

• Project/Area Number: 21500349
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Nagoya University
• Principal Investigator: SUGIMURA Yoshihisa 名古屋大学, 医学部附属病院, 講師 (50456670)
• Co-Investigator(Kenkyū-buntansha): OISO Yutaka 名古屋大学, 医学系研究科, 教授 (40203707)
• Co-Investigator(Renkei-kenkyūsha): SAWADA Makoto 名古屋大学, 環境医学研究所, 教授 (10187297)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: グリア細胞 / レーザーマイクロダイセクション / 脱髄

Research Abstract

Osmotic demyelination syndrome(ODS) is a central demyelinating disease associated with a rapid correction of hyponatremia. To clarify the role of microglia in the pathogenesis of ODS, We used laser-capture microdissection to isolate single microglia and analyzed single-microglia gene-expression profiles using high-throughput microarray assays. Our data provide a new insight into the characteristic of microglia associated with injured oligodendrocytes and new molecular targets of minocycline treatment.

Research Products

• [Journal Article] Osteoclast Response to Low Extracellular Sodium and the Mechanism of Hyponatremia-induced Bone Loss (2011)
  • Author(s): Barsony J, Sugimura Y. Verbalis JG.
  • Journal Title: J Biol Chem Volume: 25 Pages: 10864-75
  • Peer Reviewed
• [Journal Article] Poly(A) tail length of neurohypophysial hormones is shortened under endoplasmic reticulum stress (2011)
  • Author(s): Morishita Y, Arima H, Hiroi M, Hayashi M, Hagiwara D, Asai N, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Takahashi M, Oiso Y
  • Journal Title: Endocrinology Volume: 152(12) Pages: 4846-55
  • Peer Reviewed
• [Journal Article] Time-dependent Changes in Proinflammatory and Neurotrophic Responses of Microglia and Astrocytes in a Rat Model of Osmotic Demyelination Syndrome (2011)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Murase T, Ozaki N, Nagasaki H, Arima H, Murata Y, Sawada M, Oiso Y
  • Journal Title: GLIA Volume: 59 Pages: 452-62
  • Peer Reviewed
• [Journal Article] Osteoclast Response to Low Extracellular Sodium and the Mechanism of Hyponatremia-induced Bone Loss (2011)
  • Author(s): Barsony J, Sugimura Y, Verbalis JG
  • Journal Title: J Biol Chem Volume: 25 Pages: 10864-10875
  • Peer Reviewed
• [Journal Article] Poly(A) Tail Length of Neurohypophysial Hormones Is Shortened Under Endoplasmic Reticulum Stress (2011)
  • Author(s): Morishita Y, Arima H, Hiroi M, Hayashi M, Hagiwara D, Asai N, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Takahashi M, Oiso Y
  • Journal Title: Endocrinology Volume: 152 Pages: 4846-4855
  • Peer Reviewed
• [Journal Article] Minocycline prevents osmotic demyelination syndrome by inhibiting the activation of microglia (2010)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Ozaki N, Nagasaki H, Arima H, Sawada M, Oiso Y
  • Journal Title: J Am Soc Nephrol Volume: 21 Pages: 2090-8
  • Peer Reviewed
• [Journal Article] Hyponatremia-induced osteoporosis (2010)
  • Author(s): Verbalis JG, Barsony J, Sugimura Y, Tian Y, Adams D, J Carter EA, Resnick HE.
  • Journal Title: J Bone Miner Res Volume: 25 Pages: 554-63
  • Peer Reviewed
• [Journal Article] Activation of vasopressin neurons leads to phenotype progression in a mouse model for familial neurohypophysial diabetes insipid us (2010)
  • Author(s): Hiroi M, Morishita Y, Hayashi M, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Oiso Y, Arima H
  • Journal Title: J Physiol Regul Integr Comp Physiol Volume: 298 Pages: 486-93
  • Peer Reviewed
• [Journal Article] Time-dependent changes in proinflammatory and neurotrophic responses of microglia and astrocytes in a rat model of osmotic demyelination syndrome (2010)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Murase T, Ozaki N, Nagasaki H, Arima H, Murata Y, Sawada M, Oiso Y
  • Journal Title: Glia Volume: 59 Pages: 452-462
  • Peer Reviewed
• [Journal Article] Activation of vasopressin neurons leads to phenotype progression in amouse model for familial neurohypophysial diabetes insipidus. (2010)
  • Author(s): Hiroi M, Morishita Y, Hayashi M, Ozaki N, Sugimura Y, Nagasaki H, Shiota A, Oiso Y, Arima H.
  • Journal Title: Am J Physiol Regul Integr Comp Physiol 298 Pages: 486-493
  • Peer Reviewed
• [Journal Article] Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes (2009)
  • Author(s): Sugimura Y, Murase T, Oyama K, Uchida A, Sato N, Hayasaka S, Kano Y, Takagishi Y, Hayashi Y, Oiso Y, Murata Y
  • Journal Title: Diabetologia Volume: 52 Pages: 962-71
  • Peer Reviewed
• [Journal Article] Central adiponectin functions to in hibitarginine vasopressin release in consciousrats (2009)
  • Author(s): Iwama S, Sugimura Y, Murase T, Hiroi M, Goto M, Hayashi M, Arima H, Oiso Y
  • Journal Title: J Neuroendocrinol Volume: 21 Pages: 753-9
  • Peer Reviewed
• [Journal Article] Central adiponectin functions to inhibit arginine vasopressin release in conscious rats. (2009)
  • Author(s): Iwama S, Sugimura Y, Murase T, Hiroi M, Goto M, Hayashi M, Arima H, Oiso Y.
  • Journal Title: J Neuroendocrinol. 21 Pages: 753-759
  • Peer Reviewed
• [Journal Article] Prevention of neural tube defects in fetuses of streptozotocin-induced diabetic mice by loss of function of inducible nitric oxide synthase (2009)
  • Author(s): Sugimura Y, Murase T, Oyama K, Uchida A, Sato N, Hayasaka S, Kano Y, Takagishi Y, Hayashi Y, Oiso Y, Murata Y
  • Journal Title: Diabetologia 52 Pages: 962-971
  • Peer Reviewed
• [Journal Article] α-Lipoic acid reduces congenital malformations in the offspring of diabetic mice. (2009)
  • Author(s): SugimuraY, Murase T, Kobayashi K, Oyama K, Hayasaka S, Kanou Y, Oiso Y, Murata Y.
  • Journal Title: Diabetes/Metabolism Research and Reviews. 25 Pages: 287-294
  • Peer Reviewed
• [Journal Article] Folic Acid Prevents Congenital Malformations in the Offspring of Diabetic Mice. (2009)
  • Author(s): Oyama K, Sugimura Y, Murase T, Uchida A, Hayasaka S, Oiso Y, Murata Y.
  • Journal Title: Endocr J 56 Pages: 29-37
  • NAID: 10025610068
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 椙村益久 大磯ユタカ
  • Journal Title: 高ナトリウム血症 内分泌・糖尿病科(科学評論社) Pages: 393-400
• [Presentation] Pathophysiology of OsmoticDemyelination Syndrome (2011)
  • Author(s): Sugimura Y
  • Organizer: American Society of Nephrology, Kidney Week 2011(米国腎臓学会)招待講演
  • Place of Presentation: Philadelphia
  • Year and Date: 2011-11-10
• [Presentation] Pathophysiology of Osmotic Demyelination Syndrome (2011)
  • Author(s): Sugimura Y
  • Organizer: American Society of Nephrology, Kidney Week 2011(米国腎臓学会)
  • Place of Presentation: Philadelphia, USA(招待講演)
  • Year and Date: 2011-11-10
• [Presentation] Minocycline Prevents Osmotic Demyelination Syndrome by Inhibiting the Activation of Microglia (2011)
  • Author(s): Sugimura Y
  • Organizer: 9th World Congress on Neurohypophysial Hormones(国際神経下垂体学会),招待講演
  • Place of Presentation: Boston
  • Year and Date: 2011-07-29
• [Presentation] Minocycline Prevents Osmotic Demyelination Syndrome by Inhibiting the Activation of Microglia (2011)
  • Author(s): Sugimura Y
  • Organizer: 9th World Congress on Neurohypophysial Hormones(国際神経下垂体学会)
  • Place of Presentation: Boston, USA(招待講演)
  • Year and Date: 2011-07-29
• [Presentation] COMPARATIVE ANALYSIS OF GENE EXPRESSION PROFILES OF MICROGLIA FROM DEMYELINATIVE LESIONS INOSMOTIC DEMYELINATION SYNDROME RAT MODEL (2010)
  • Author(s): Haruyuki Suzuki, YoshihisaSugimura, Shintaro Iwama, HiromiSuzuki, Atsushi Kiyota, MakotoSawada, Yutaka Oiso
  • Organizer: 10^<th> International Congress of Neuroimmunology
  • Place of Presentation: Barcelona, Spain
  • Year and Date: 2010-10-27
• [Presentation] Gene expression profiling of microglia from the demyelinative lesions in osmotic demyelination syndrome (2010)
  • Author(s): Sugimura Y, Suzuki H, Iwama S, Suzuki H, Kiyota A, Sawada M, Oiso Y
  • Organizer: Neuro2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-09-02
• [Presentation] The pathogenes is of osmotic demyelination syndrome(ODS) and the therapy of ODS (2010)
  • Author(s): Sugimura Y
  • Organizer: 14^<th> International Congress of Endocrinology(ICE2010)(国際内分泌学会)招待講演
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-27
• [Presentation] The differing roles of nitric oxide synthase isoforms in osmotic demyelination syndrome inrats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Suzuki H, Iwama S, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] Protective effects of minocycline on osmotic demyelination syndrome in rats (2009)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Arima H, Sawada M, OisoY
  • Organizer: Neuroscience
  • Place of Presentation: Chicago
  • Year and Date: 2009-10-19
• [Presentation] The differing roles of nitric oxide synthase isoforms in osmotic demyelination syndrome in rats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Suzuki H, Iwama S, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: Neuroscience 2009
  • Place of Presentation: シカゴ
  • Year and Date: 2009-10-18
• [Presentation] Protective effects of minocycline on osmotic demyelination syndrome in rats (2009)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Arima H, Sawada M, Oiso Y
  • Organizer: Neuroscience 2009
  • Place of Presentation: シカゴ
  • Year and Date: 2009-10-18
• [Presentation] The differing pathological effects of nitric oxide synthase is forms in osmotic demyelination syndrome in rats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Iwama S, Suzuki H, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: The 32nd ANNUAL MEETING OF THEJAPAN NEUROSCIENCE SOCIETY
  • Place of Presentation: 名古屋
  • Year and Date: 2009-09-17
• [Presentation] The differing pathological effects of nitric oxide synthase isoforms in osmotic demyelination syndrome in rats (2009)
  • Author(s): Sugimura Y, Murase T, Takefuji S, Takagi H, Iwama S, Suzuki H, Takagishi Y, Arima H, Sawada M, Oiso Y, Murata Y
  • Organizer: The 32nd ANNUAL MEETING OF THE JAPAN NEUROSCIENCE SOCIETY
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-09-17
• [Presentation] Proinflammatory and Neurotrophic Responses of Microglia and Astrocytes in a RatModel for Osmotic Demyelination Syndrome (2009)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Arima H, Murata Y, Sawada M, Oiso Y
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: Kitakyushu
  • Year and Date: 2009-09-07
• [Presentation] Minocycline prevents osmotic demyelination by inhibiting the activation of microglia in rats (2009)
  • Author(s): Suzuki H, Sugimura Y, Iwama S, Suzuki H, Arima H, Sawada M, Oiso Y
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: Kitakyushu
  • Year and Date: 2009-09-07
• [Presentation] Hyponatremia-Induced Osteoporosis (2009)
  • Author(s): J.Barsony, Sugimura, Y.Manigrasso, H.H.Tam, Q.Xu, Y.Tian, D.Adams, E.A.Carter, H.E.Regnick, J.G.Verbalis
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-09-07
• [Presentation] Proinflammatory and Neurotrophic Responses of Microglia and Astrocytes in a Rat Model for Osmotic Demyelination Syndrome (2009)
  • Author(s): Iwama S, Sugimura Y, Suzuki H, Suzuki H, Arima H, Muratay, Sawada M, Oiso Y
  • Organizer: 8th World Congress on Neurohypoohysial Hormones
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-09-05
• [Presentation] リンパ球性漏斗下垂体炎のバイオマーカー開発-プロテオーム解析による病因自己抗原分子の検索- (2009)
  • Author(s): 椙村益久
  • Organizer: 第36回日本神経内分泌学会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-09-05
• [Presentation] プロテオミクスによるリンパ球性漏斗下垂体後葉炎の病因自己抗原の検索 (2009)
  • Author(s): 岩間信太郎, 椙村益久, 加藤琢哉, 鈴木陽之, 榎本篤, 有馬寛, 大磯ユタカ
  • Organizer: 第36回日本神経内分泌学会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-09-04
• [Presentation] SIADHモデルラットにおいてミノサイクリンは浸透圧性脱髄の発症・進展を防止する (2009)
  • Author(s): 鈴木陽之, 椙村益久, 岩間信太郎, 鈴木弘美, 有馬寛, 澤田誠, 大磯ユタカ
  • Organizer: 第36回日本神経内分泌学会
  • Place of Presentation: 北九州国際会議場(福岡県)
  • Year and Date: 2009-09-04
• [Presentation] アディポネクチンによるバゾプレシン分泌抑制作用機序の検討 (2009)
  • Author(s): 岩間信太郎, 椙村益久, 村瀬孝司, 有馬寛, 大磯ユタカ
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(群馬県)
  • Year and Date: 2009-04-25
• [Presentation] 浸透圧性脱髄症候群ラットモデルにおけるミノサイクリンの検討 (2009)
  • Author(s): 鈴木陽之,椙村益久,岩間信太郎,有馬寛,大磯ユタカ
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬
  • Year and Date: 2009-04-23
• [Presentation] 浸透圧性脱髄症候群ラットモデルにおけるミノサイクリンの検討 (2009)
  • Author(s): 鈴木陽之, 椙村益久, 岩間信太郎, 有馬寛, 大磯ユタカ
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(群馬県)
  • Year and Date: 2009-04-23
• [Book] ADH(AVP)不適切分泌症候群、症候群ハンドブック403 (2011)
  • Author(s): 椙村益久、大磯ユタカ
  • Total Pages: 403
  • Publisher: 中山書店
• [Book] 高ナトリウム血症内分泌・糖尿病科 (2009)
  • Author(s): 椙村益久、大磯ユタカ
  • Publisher: 科学評論社