| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Research Abstract |
The purpose of this study is to analyze the function of neuron-specific serine/threonine kinase Cyclin-dependent kinase 5(Cdk5) by using conditional KO mice of its activating subunit p35 trough the behavioral analysis such as spatial learning and fear conditioning test, and electrophysiological analysis and biochemical analysis. In this study we generated p35-flox mice in which two loxP sequences were inserted in p35 gene allele. We generated inducible p35 conditional KO by crossing p35-flox and CreER mice in which Cre is activated by Tamoxifen(TM) and p35 gene was destructed after brain formed. We confirmed decrease of p35 protein by p. o. administration of 2 mg TM for three days. We also generated CA1-specific p35 conditional KO mice by crossing CaMKII-Cre mice, and confirmed decreased level of p35 protein in hippocampus. We observed decreased numbers of dendritic spines in the hippocampal CA1 pyramidal neurons in CA1-p35 cKO mice. Using these p35 conditional KO mice, we currently conduct behavioral analysis of p35 cKO mice.
|
