Biosynthetic pathway to neuromelanin and its structural modification in aging : Incorporation of neurotoxic molecules in neuromelanin

• Project/Area Number: 21500358
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Fujita Health University
• Principal Investigator: WAKAMATSU Kazumasa 藤田保健衛生大学, 医療科学部, 教授 (80131259)
• Co-Investigator(Kenkyū-buntansha): ITO Shosuke 藤田保健衛生大学, 名誉教授 (70121431) ; KATO Yoshimitsu 藤田保健衛生大学, 医療科学部, 准教授 (20121436)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ニューロメラニン / 化学分析 / パーキンソン病 / ドーパミンメラニン / システイニルドーパミンメラニン / 黒質 / 青斑核 / カテコールアミン作動性ニューロン / ユーメラニン / フェオメラニン / ドーパミン / システイニルドーパミン

Research Abstract

The biosynthesis and structure of neuromelanin(NM) still remains poorly understood. Using model compounds of the melanic component of NM prepared by tyrosinase oxidation at various ratios of dopamine(DA) and cysteine(Cys) under physiological conditions, we performed a biosynthetic and biodegradative(aging) study by following the time course of oxidation to NM and the subsequent biodegradation of NM under various heating conditions. By following the time course of the biosynthetic pathway of synthetic NM we found that the neurotoxic molecules are trapped in NM. By comparing the ratios of the degradation markers, natural NM was found to be similar to aged(heated) NM prepared from a 2 : 1 molar ratio of DA and Cys, which can be used as a model of natural NM. A method to prepare synthetic NM that mimics the melanic component of human NM structurally was now established, i. e., tyrosinase oxidation of DA+Cys in a ratio of 2 : 1 followed by heating at 37℃for 40 days. This preparation of model NM should facilitate studies elucidating the physiological roles of NM during the aging process and its roles in the etiology pathogenesis of PD.

Research Products

• [Journal Article] Oculocutaneous albinism 1 minimal pigment type : a case report on the analysis of genotype of an OCA1MP patient (2012)
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• [Journal Article] Withania somnifera extract attenuates stem cell factor-stimulated pigmentation in human epidermal equivalents through interruption of ERK phosphorylation within melanocytes (2012)
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  • Journal Title: J.Nat.Med Volume: 66 Pages: 435-446
  • NAID: 10031087576
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• [Journal Article] Age-dependent changes in eumelanin composition in hairs of various origins (2012)
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  • Journal Title: J.Cosmetic.Sci. Volume: 34 Issue: 1 Pages: 102-107
  • DOI: 10.1111/j.1468-2494.2011.00691.x
  • Peer Reviewed
• [Journal Article] Diversity of human hair pigmentation as studied by chemical analysis of eumelanin and pheomelanin (2011)
  • Author(s): Ito, S., Wakamatsu, K.
  • Journal Title: J. Eur. Acad. Dermatol. Venereol Volume: 25 Pages: 1369-1380
  • Peer Reviewed
• [Journal Article] Usefulness of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin in various tissue samples : application to chemical analysis of human hair melanins (2011)
  • Author(s): Ito, S., Nakanishi, Y., Valenzuela, R. K., Brilliant, M. H., Kolbe, L., Wakamatsu, K.
  • Journal Title: Pigment Cell Melanoma Res Volume: 24 Pages: 605-618
  • Peer Reviewed
• [Journal Article] Maintenance of immune hyporesponsiveness to melanosomal proteins by DHICA-mediated antioxidation : possible implications for autoimmune vitiligo (2011)
  • Author(s): Liu, X-M., Zhou, Q., Xu, S-Z., Wakamatsu, K., Lei, T-C.
  • Journal Title: Free Radic. Biol. Med Volume: 50 Pages: 1177-1185
  • Peer Reviewed
• [Journal Article] Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation (2011)
  • Author(s): Hellstrom, A. R., Watt, B., Fard, S. S., Tenza, D., Mannstriim, Narfstriim, BjOn Ekesten, Ito, S., Wakamatsu, K., Larsson, J., Ulfendahl, M., Kullander, K., Raposo, G., Kerje, S., Hallbiiiik, Marks, M. S., Andersson, L.
  • Journal Title: PLoS Genetics Volume: 7
  • Peer Reviewed
• [Journal Article] Diversity of human hair pigmentation as studied by chemical analysis of eumelanin and pheomelanin (2011)
  • Author(s): Ito, S., Wakamatsu, K
  • Journal Title: J.Eur.Acad.Dermatol.Venereol. Volume: 25 Issue: 12 Pages: 1369-1380
  • DOI: 10.1111/j.1468-3083.2011.04278.x
  • Peer Reviewed
• [Journal Article] Usefulness of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin in various tissue samples : application to chemical analysis of human hair melanins (2011)
  • Author(s): Ito, S., Nakanishi, Y., Valenzuela, R.L., Brilliant, M.H, et al
  • Journal Title: Pigment Cell Melanoma Res Volume: 24 Issue: 4 Pages: 605-613
  • DOI: 10.1111/j.1755-148x.2011.00864.x
  • Peer Reviewed
• [Journal Article] Maintenance of immune hyporesponsiveness to melanosomal proteins by DHICA-mediated antioxidation : possible implications for autoimmune vitiligo (2011)
  • Author(s): Liu, X-M., Zhou, Q., Xu, S-Z., Wakamatsu, K., et al
  • Journal Title: Free Radic.Biol.Med. Volume: 50 Issue: 9 Pages: 1177-1185
  • DOI: 10.1016/j.freeradbiomed.2011.01.017
  • Peer Reviewed
• [Journal Article] Melanin-based coloration predicts aggressiveness and boldness in captive eastern Hermann's tortoises (2011)
  • Author(s): Mafli, A., Wakamatsu, K., Roulin, A
  • Journal Title: Animal Behaviour Volume: 81 Issue: 4 Pages: 859-863
  • DOI: 10.1016/j.anbehav.2011.01.025
  • Peer Reviewed
• [Journal Article] An extract of Melia toosendan attenuates endothelin-1-stimulated pigmentation in human epidermal equivalents through the interruption of PKC activity with melanocytes (2011)
  • Author(s): Nakajima, H., Wakabayashi, Y., Wakamatsu, K., Imokawa, G.
  • Journal Title: Arch Dermatol Res Volume: 303 Issue: 4 Pages: 263-276
  • DOI: 10.1007/s00403-011-1143-y
  • Peer Reviewed
• [Journal Article] An extract of withania somnifera attenuates endothelin-1-stimulated pigmentation in human epidermal equivalents through the interruption of PKC activity within melanocytes (2011)
  • Author(s): Nakajima, H., Wakabayashi, Y., Wakamatsu, K., Imokawa, G.
  • Journal Title: Phytother.Res Volume: 25 Issue: 9 Pages: 1398-1411
  • DOI: 10.1002/ptr.3552
  • Peer Reviewed
• [Journal Article] Inactivation of Pmel alters melanosome shape but has only a subtle effect on visible pigmentation (2011)
  • Author(s): Hellstrom, A.R., Watt, B., Fard, S.S., Tenza, D., et al
  • Journal Title: Plos Genetics Volume: 7 Issue: 9 Pages: e1002285-e1002285
  • DOI: 10.1371/journal.pgen.1002285
  • Peer Reviewed
• [Journal Article] A novel deletion mutation of mouse ruby-eye 2 named ru2d/Hps5ru2-d inhibits melanocyte differentiation and its impaired differentiation is rescued by L-tyrosine (2011)
  • Author(s): Tomohisa Hirobe
  • Journal Title: Zool. Sci. Volume: 28 Issue: 11 Pages: 790-801
  • DOI: 10.2108/zsj.28.790
  • Peer Reviewed
• [Journal Article] 1-(2,4-Dihydroxyphenyl)-3-(2,4-dimethoxy-3-methylphenyl) propane inhibits melanin synthesis by dual mechanism (2011)
  • Author(s): Niki, Y., Yoshida, M., Ando, H., Wakamatsu, K., et al
  • Journal Title: J.Dermatol.Sci. Volume: 63 Pages: 115-121
  • DOI: 10.1016/j.jdermsci.2011.04.002
  • Peer Reviewed
• [Journal Article] Spiny mice modulate eumelanin to pheomelanin ratio to achieve cryptic coloration in "Evolution Canyon" (2010)
  • Author(s): Singaravelan, N., Pavlicek, T., Beharav, A., Wakamatsu, K., Ito, S., Nevo, E.
  • Journal Title: Israel. Plos One Volume: 5
  • Peer Reviewed
• [Journal Article] Protection against ultraviolet radiation involves MC1R-mediated non-pigmentary and pigmentary mechanisms in vivo (2010)
  • Author(s): Robinson, S., Dixon, S., August, S., Diffey, B., Wakamatsu, K., Ito, S., Friedmann, P. S., Healy, E.
  • Journal Title: J. Invest. Dermatol Volume: 130 Pages: 1904-1913
  • Peer Reviewed
• [Journal Article] Regulation of DHICA-mediated antioxidation by dopachrome tautomerase : implication for skin pthotoprotection against UVA radiation (2010)
  • Author(s): Jiang, S., Liu, X-M., Dai, X., Zhou, Q., Lei, T-C., Beermann, F., Wakamatsu, K., Xu, S-Z.
  • Journal Title: Free Rad. Biol. Med Volume: 48 Pages: 1144-1151
  • Peer Reviewed
• [Journal Article] Imaging, chemical and spectroscopic studies of the methylation-induced decomposition of melanosome (2010)
  • Author(s): Kempf, V., Wakamatsu, K., Ito, S., Simon, JD.
  • Journal Title: Photochem Photobiol Volume: 86 Pages: 765-771
  • Peer Reviewed
• [Journal Article] Agaritine purified from Agaricus blazei Murrill exerts anti-tumor activity against leukemic cells (2010)
  • Author(s): Endo, M., Beppu, H., Akiyama, H., Wakamatsu, K., Ito, S., Kawamoto, Y., Shimpo, K., Sumiya, T., Koike, T., Matsui, T.
  • Journal Title: Biochim Biophys Acta Pages: 669-673
  • Peer Reviewed
• [Journal Article] Melanocortin 1 receptor genotype : an important determinant of the damage response of Melanocytes to ultraviolet radiation (2010)
  • Author(s): Kadekaro, AL., Leachman, S., Kavanagh, RJ., Swope, V., Cassidy, P., Supp, D., Sartor, M., Schwemberger, S., Babcock, G., Wakamatsu, K., Ito, S., Koshoffer, A., Boissy, RE., Manga, P., Sturm, RA., Abdel-Malek, ZA.
  • Journal Title: The FASEB J Volume: 24 Pages: 3850-3860
  • Peer Reviewed
• [Journal Article] Increased cysteinyldopa plasma levels hint to melanocyte as stress sensor in psoriasis (2010)
  • Author(s): Panzella, L., Wakamatsu, K., Monfrecola, G., Ito, S., Ayala, F., Napolitano, A.
  • Journal Title: Experimental Dermatology Volume: 20 Pages: 288-290
  • Peer Reviewed
• [Journal Article] Human hair melanins : What we have learned and have not learned from mouse coat color pigmentation (2010)
  • Author(s): Ito, S., Wakamatsu, K.
  • Journal Title: Pigment Cell & Melanoma Res Volume: 24 Pages: 63-74
  • Peer Reviewed
• [Journal Article] What we have learned and have not learned from mouse coat color pig-mentation (2010)
  • Author(s): Ito, S
  • Journal Title: Pigment Cell & Melanoma Res Volume: 24 Pages: 63-74
  • Peer Reviewed
• [Journal Article] Spiny mice modulate eumelanin to pheomelanin ratio to achieve cryptic coloration in "Evolution Canyon," Israel. (2010)
  • Author(s): Singaravelan, N
  • Journal Title: Plos One Volume: 5
  • Peer Reviewed
• [Journal Article] Predicting phenotype from genotype : normal pigmentation. (2010)
  • Author(s): Valenzuela, R.K.
  • Journal Title: J.Forensic Sci Volume: 55 Pages: 315-322
  • Peer Reviewed
• [Journal Article] protection against ultraviolet radiation involves MC1R-mediated non-pigmentary and pigmentary mechanisms in vivo (2010)
  • Author(s): Robinson, S
  • Journal Title: J.Invest.Dermatol. Volume: 130 Pages: 1904-1913
  • Peer Reviewed
• [Journal Article] A close relationship between androgen levels and eumelanogenesis in the teleost red seabream (Pagrus major) : Quantitative analysis of its se-asonal variation and effects of oral treatment with methyl testosterone (2010)
  • Author(s): Adachi, K
  • Journal Title: Comp.Biochem.Physiol.Part A : Mol.Integr.Physiol. Volume: 156 Pages: 184-189
  • Peer Reviewed
• [Journal Article] Regulation of DHICA-mediated antioxidation by dopachrome tautomerase : implication for skin photoprotection against UVA radiation (2010)
  • Author(s): Jiang, S
  • Journal Title: Free Rad.Biol.Med Volume: 48 Pages: 1144-1151
  • Peer Reviewed
• [Journal Article] Estrogen Increases Hair Pigmentation in Female Recessive Yellow Mice (2010)
  • Author(s): Hirobe, T
  • Journal Title: Zoological Sci. Volume: 27 Pages: 470-476
  • Peer Reviewed
• [Journal Article] Regioselectivity on the cooxidation of 5,6-dihydroxyindole and its 2-carbo-xylic derivative from the quantum chemical calculations (2010)
  • Author(s): Okuda, H
  • Journal Title: Chemical Physics Letters Volume: 490 Pages: 226-229
  • Peer Reviewed
• [Journal Article] Agaritine purified from Agaricus blazei Murrill exerts anti-tumor activity against leukemic cells (2010)
  • Author(s): Endo, M
  • Journal Title: Biochim Biophys Acta Volume: 1800 Pages: 669-673
  • Peer Reviewed
• [Journal Article] Melanoma-targeted chemo-thermo-immuno (CTI)-therapy using N-propion-yl-4-S-cysteaminylphenol-magnetite nanoparticles elicits CTL response via heat shock protein-peptide complex release (2010)
  • Author(s): Sato, A
  • Journal Title: Cancer Sci Volume: 101 Pages: 1936-1946
  • Peer Reviewed
• [Journal Article] Ultraviolet absorption coefficients of melanosomes containing eumelanin as related to the relative content of DHI and DHICA (2010)
  • Author(s): Peles, DN
  • Journal Title: J.Phys.Chem.Lett Volume: 1 Pages: 2391-2395
  • Peer Reviewed
• [Journal Article] Increased cysteinyldopa plasma levels hint to melanocyte as stress sens or in psoriasis (2010)
  • Author(s): Panzella, L
  • Journal Title: Experimental Dermatology Volume: 20 Pages: 278-296
  • Peer Reviewed
• [Journal Article] The mouse pink-eyed dilution allele of the P-gene greatly inhibits eume-lanin, but not pheomelanin synthesis as studied by chemical analysis (2010)
  • Author(s): Hirobe, T
  • Journal Title: Pigment Cell & Melanoma Res Volume: 24 Pages: 241-246
  • Peer Reviewed
• [Journal Article] Chemical analysis of late stages of phaeomelanogenesis : conversion of dihydrobenzothiazine to a benzothiazole structure (2009)
  • Author(s): Wakamatsu, K., Ohtara, K., Ito, S.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 474-486
  • Peer Reviewed
• [Journal Article] Melanins seem to be present everywhere in the body, but for what? (2009)
  • Author(s): Ito, S.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 12-13
  • Peer Reviewed
• [Journal Article] Prolonged treatment of fair-skinned mice with topical forskolin causes persistent tanning and UV protection (2009)
  • Author(s): Spry, ML., Vanover, JC., Scott, T., Abona-Ama, 0., Wakamatsu, K., Ito, S., D' Orazio, JA.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 219-229
  • Peer Reviewed
• [Journal Article] Isomeric cysteinyldopas provide a(photo) degradable bulk component and a robust structural element in red human hair pheomelanin (2009)
  • Author(s): Greco, G., Wakamatsu, K., Panzella, L., Ito S., Napolitano, A., d' Ischia, M.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 319-327
  • Peer Reviewed
• [Journal Article] Comparison of eumelanin and pheomelanin content between cultured uveal melanoma cells and normal uveal melanocytes (2009)
  • Author(s): Hu, DN., Wakamatsu, K., Ito, S., McCormick, SA.
  • Journal Title: Melanoma Res Volume: 19(2) Pages: 75-79
  • Peer Reviewed
• [Journal Article] Current Challenges in understanding melanogenesis : Bridging chemistry, biological control, morphology and function (2009)
  • Author(s): Simon, J. D., Peles, D., Wakamatsu, K., Ito, S.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 563-579
  • Peer Reviewed
• [Journal Article] Stem cell factor rescues tyrosinase expression and pigmentation in discreet anatomic locations in albino mice (2009)
  • Author(s): Vanover, J. C., Spry, M. L., Hamilton, L., Wakamatsu, K., Ito, S., D' Orazio, J. A.
  • Journal Title: Pigment Cell Melanoma Res Volume: 22 Pages: 827-838
  • Peer Reviewed
• [Journal Article] Chemical analysis of late stages of phaeomelanogenesis : conversion of dihydrobenzothiazine to a benzothiazole structure (2009)
  • Author(s): Wakamatsu, K
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 474-486
  • Peer Reviewed
• [Journal Article] Prolonged treatment of fair-skinned mice with topical forskolin causes persistent tanning and UV protection (2009)
  • Author(s): Spry, ML
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 219-229
  • Peer Reviewed
• [Journal Article] Isomeric cysteinyldopas provide a(photo)degradable bulk component and a robust structural element in red human hair pheomelanin (2009)
  • Author(s): Greco, G
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 319-327
  • Peer Reviewed
• [Journal Article] Comparison of eumelanin and pheomelanin content between cultured uveal melanoma cells and normal uveal melanocytes (2009)
  • Author(s): Hu, DN
  • Journal Title: Melanoma Res 19 Pages: 75-79
  • Peer Reviewed
• [Journal Article] Agouti protein, mahogunin and attractin in phaeomelanogenesis and melanoblast-like alteration of Melanocytes : a camp-independent pathway (2009)
  • Author(s): Hida, T
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 623-634
  • Peer Reviewed
• [Journal Article] Strengh and cost of an induced immune response are associated with a heritable melanin-based colour trait in female tawny owls (2009)
  • Author(s): Gasparini, J
  • Journal Title: J.Animal Ecology 78 Pages: 608-616
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• [Journal Article] Purification and growth of melanocortin 1 receptor(Mc1r)-defective primary murine Melanocytes is dependent on stem cell factor from keratinocytes-conditioned media (2009)
  • Author(s): Scott, T
  • Journal Title: In Vitro Cellular & Developmental Biology-Animal 45 Pages: 577-583
  • Peer Reviewed
• [Journal Article] Current Challenges in understanding melanogenesis : Bridging chemistry, biological control, morphology and function (2009)
  • Author(s): Simon, JD
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 563-579
  • Peer Reviewed
• [Journal Article] Independent regulation of hair and skin color by two G protein-coupled pathway (2009)
  • Author(s): Van Raamsdonk, CD
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 819-826
  • Peer Reviewed
• [Journal Article] Serum levels of 5-S-cysteinyldopa are correlated with skin colors in hemodialysis patients but not in peritoneal dialysis patients (2009)
  • Author(s): Murakami, K
  • Journal Title: Blood Purif 28 Pages: 209-215
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• [Journal Article] Short- and long-term effects of UV radiation on the pigmentation of human skin (2009)
  • Author(s): Coelho, SG
  • Journal Title: J Invest Dermatol Symp Proc 14 Pages: 32-35
  • Peer Reviewed
• [Journal Article] Stem cell factor rescues tyrosinase expression and pigmentation in discreet anatomic locations in albino mice (2009)
  • Author(s): Vanover, J.C.
  • Journal Title: Pigment Cell Melanoma Res 22 Pages: 827-838
  • Peer Reviewed
• [Journal Article] N-propionyl-cysteaminylphenol-magnetite(NPrCAP/M)is a nanoparticle for the targeted growth suppression of melanoma cells (2009)
  • Author(s): Sato, M
  • Journal Title: J Invest Dermatol 129 Pages: 2233-2241
  • Peer Reviewed
• [Journal Article] Growth inhibition of re-challenge B16 melanoma transplant by conjugates of melanogenesis substrate and magnetite nanoparticles as the basis for developing melanoma-targeted chemo-thermo-immunotherapy (2009)
  • Author(s): Takada, T
  • Journal Title: J Biomed Biotechnol
  • Peer Reviewed
• [Journal Article] Melanin-based iridescent feather color in the Jungle Crow (2009)
  • Author(s): Lee, E
  • Journal Title: J.Vet.Med.Sci 71 Pages: 1261-1263
  • Peer Reviewed
• [Journal Article] メラノーマ形質を分子標的とした化学・温熱・免疫療法の基礎と臨床 (2009)
  • Author(s): 神保孝一
  • Journal Title: Skin Cancer 24 Pages: 174-180
  • Peer Reviewed
• [Journal Article] マウス大腸粘膜の細胞増殖能に対するアロエエモジン5週間混餌投与の影響 (2009)
  • Author(s): 新保寛
  • Journal Title: 医学と生物学 153 Pages: 218-224
  • Peer Reviewed
• [Presentation] Neuromelanins in brain aging and Parkinson's disease (2011)
  • Author(s): Zecca, L., Zhang, W., Jackson-Lewis, S., Przedborski, S., et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-23
• [Presentation] Introduction of melanoma in situ peptide vaccine by chemotherapy through exploitation of melanogenesis substrate, NPrCAP, and its conjugation with magnetite nanoparticles (2011)
  • Author(s): Jimbow, K., Yoneda, A., Tamura, Y., Osai, Y., Sato, M., et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-22
• [Presentation] N-Propionyl-4-S-Cysteaminylphenol generates reactive oxygen species and mediates apoptosis in pigmented melanoma cells (2011)
  • Author(s): S.Commo, K.Wakamatsu, S.Ito
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-22
• [Presentation] Distribution patterns of eumelanin and pheomelanin in human skin (2011)
  • Author(s): S.G.Coelho, S.Ito, K.Wakamatsu, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-22
• [Presentation] Chemistry and Biophysics of neuromelanin (2011)
  • Author(s): Wakamatsu, K., Murase, T., Zucca, F.A., Zecca, L. Ito, S.
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France(招待講演)
  • Year and Date: 2011-09-21
• [Presentation] Evaluation of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin (2011)
  • Author(s): Ito, S., Nakanishi, Y., Wakamatsu, K
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-21
• [Presentation] Quantum chemical study of 5,6-dihydroxyindole tetramers as eumelanin model molecules (2011)
  • Author(s): H.Okuda, T.Sota, K.Koike, K.Monda, T.Nakamura, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2011-09-21
• [Presentation] Chemistry and biophysics of neuromelanin (2011)
  • Author(s): Wakamatsu, K., Murase, T., Zucca, F. A., Zecca, L., Ito, S.
  • Organizer: XXlst International Pigment Cell Conference(IPCC)
  • Place of Presentation: Bordeaux, France
• [Presentation] Evaluation of alkaline hydrogen peroxide oxidation to analyze eumelanin and pheomelanin (2011)
  • Author(s): Ito, S., Nakanishi, Y., Wakamatsu, K.
  • Organizer: XXlst International Pigment Cell Conference(IPCC)
  • Place of Presentation: Bordeaux, France
• [Presentation] Human hair pigmentation characteristics revealed by melanin determination in human eumelanic hairs of various ethnic origins (2011)
  • Author(s): Commo, S., Wakamatsu, K., Ito, S.
  • Organizer: XX 1 st International Pigment Cell Conference(IPCC)
  • Place of Presentation: Bordeaux, France
• [Presentation] A novel pathway for regulation of pigmentation by glutamate receptor mGluR6 through its action on TRPM1 (2011)
  • Author(s): Devi, S., Markandeya, Y., Maddodi, N., Wakamatsu, K., Ito, S., Balijepalli, R., Setaluri, V.
  • Organizer: XXlst International Pigment Cell Conference(IPCC)
  • Place of Presentation: Bordeaux, France
• [Presentation] Development and evaluation of antitumor effect of novel NPrCAP-magnetite nanoparticles for chemo-thermo-immunotherapy in malignant melanoma (2011)
  • Author(s): Yoneda, A., Tamura, Y., Nohara, A., Ito, A., Honda, H., et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] Human hair pigmentation characteristics revealed by melanin determination m human eumelanic hairs of various ethnic origins (2011)
  • Author(s): Y.Ishii-Osai, T.Yamashita, M.Okura, Y.Tamura, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] A novel pathway for regulation of pigmentation by glutamate receptor mGluR6 through its action on TRPM1 (2011)
  • Author(s): S.Devi, Y.Markandeya, N.Maddodi, K.Wakamatsu, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] Slaty mutation inverses the ratio of DHI to DHICA content of eumelanin in both mouse melanocytes and hair (2011)
  • Author(s): Y.Niki, T.Hirobe, K.Wakamatsu, H.Ando, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] Oculocutaneous albinism 1 minimal pigment type ; a case report on the analysis of genotype-phenotype correlation (2011)
  • Author(s): M.Kono, T.Kondo, S.Ito, T.Suzuki, K.Wakamatsu, et al
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] Serum 5-S-cysteinyldopa levels in psoriasis and vitiligo patients undergoing narrowband ultraviolet B phototherapy (2011)
  • Author(s): K.Kikuchi, K.Wakamatsu, Y.Tada, S.Ito
  • Organizer: XX1st International Pigment Cell Conference
  • Place of Presentation: Bordeaux, France
• [Presentation] ヒト脳内黒質に存在する黒褐色色素ニューロメラニンの生成過程の解明 (2010)
  • Author(s): 村瀬貴也、伊藤祥輔、若松一雅
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-28
• [Presentation] 「分かりやすいメラニンの生化学とメラノサイトの生化学」混合型メラニン形成の生化学 (2010)
  • Author(s): 伊藤祥輔
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-28
• [Presentation] 子化学計算からの5, 6-dihydroxyindoleとそのカルボキシ誘導体のヘテロニ量体化機構 (2010)
  • Author(s): 奥田英和、若松一雅、伊藤祥輔、宗田孝之
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] グルコサミンは3次元ヒト表皮モデルでエンドセリンー1/ステムセルファクター誘導色素沈着をメラノサイトでのCREBシグナルの減少により抑制する (2010)
  • Author(s): 若林雄希、中島弘明、深澤勝則、若松一雅、芋川玄爾
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] ルビーアイ2の新規突然変異遺伝子(ru2d/Hps5d)はマウスのメラノサイトの分化を抑制し毛色を薄くするが、L-チロシンはその分化活性を回復させる (2010)
  • Author(s): 広部知久、吉原千尋、竹内栄、阿部宏之、若松一雅、伊藤祥輔、河陽子、相馬良直、溝口昌子
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 量子化学計算からの5,6-dihydroxyindoleとその2-カルボキシ誘導体のヘテロ二量体化機構 (2010)
  • Author(s): 奥田英和
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] グルコサミンは3次元ヒト表皮モデルでエンドセリン-1/ステムセルファクター誘導色素沈着をメラノサイトでのCREBシグナルの減少により抑制する (2010)
  • Author(s): 若林雄希
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] 尋常性白斑患者におけるNarrowband UVB治療前後の血清5-S-cysteinyldopa値について (2010)
  • Author(s): 菊池かな子
  • Organizer: 第23回日本色素細胞学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-27
• [Presentation] Human hair pigmentation : Lessons from mouse hair pigmentation (2010)
  • Author(s): Ito, S
  • Organizer: Asian Society for Pigment Cell Research
  • Place of Presentation: Guangzhou, China(招待講演)
  • Year and Date: 2010-06-14
• [Presentation] Estrogen increases the hair pigmentation in female recessive yellow mice (2010)
  • Author(s): Hirobe, T
  • Organizer: Asian Society for Pigment Cell Research
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2010-06-14
• [Presentation] Serum 5-S-cysteinyldopa levels in psoriasis and vitiligo patients undergoing narrow band ultraviolet B phototherapy (2010)
  • Author(s): Kikuchi, K
  • Organizer: Asian Society for Pigment Cell Research
  • Place of Presentation: Guangzhou, China
  • Year and Date: 2010-06-14
• [Presentation] Melanogenesis cascade for developing novel selective drug delivery and chemo-thermo-immunotherapeutic strategies in melanoma ; specificity and biological effect (2009)
  • Author(s): Jimbow, K
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-06
• [Presentation] フェオメラニン生成の後期過程の化学的分析 (2009)
  • Author(s): 若松一雅、太田良和美、伊藤祥輔
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] 5, 6-dihydroxyindole-2-carboxylicacid二量体の異性体判別:中赤外吸収スペクトル (2009)
  • Author(s): 奥田秀和、中村厚、若松一雅、伊藤祥輔、宗田孝之
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] N-propionyl-4-S-cysteaminylphenolはメラノーマ細胞にアポトーシスを誘導しマウスB16F1腫瘍の増殖を抑制する (2009)
  • Author(s): 箴井泰江、田村保明、佐藤昇志、若松一雅、伊藤祥輔、井藤彰、本多裕之、黄倉真恵、佐藤亜紀子、山下利春、神保孝一
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] 5,6-dihydroxyindole-2-carboxylic acid 二量体の異性体判別:中赤外吸収スペクトル (2009)
  • Author(s): 奥田秀和
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] N-propionyl-4-S-cysteaminylphenol はメラノーマ細胞にアポトーシスを誘導しマウスB16F1腫瘍の増殖を抑制する (2009)
  • Author(s): 筬井泰江
  • Organizer: 第22回日本色素細胞学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-12-05
• [Presentation] N-propionyl-cysteaminylphenol suppresses re-challenge of mouse B16F1 tumor by inducing tumor-specific immune response (2009)
  • Author(s): 筬井泰江
  • Organizer: 39^<th> Annual Meeting of the European Society for Dermatological Research
  • Place of Presentation: Budapest, Hungary
  • Year and Date: 2009-09-09
• [Presentation] Chemical structure of neuromelanin : an elucidation to the production process (2009)
  • Author(s): Wakamatsu, K.
  • Organizer: 3rd Asian Society for Pigment Cell Research Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-06-12
• [Presentation] Chemical structure of neuromelanin : an elucidation to the production process (2009)
  • Author(s): 若松一雅
  • Organizer: 3^<rd> Asian Society for Pigment Cell Research Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-06-12
• [Presentation] Chemical and biochemical control of mixed melanogenesis (2009)
  • Author(s): Ito, S.
  • Organizer: 3rd Asian Society for Pigment Cell Research Meeting
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-06-11
• [Presentation] Serum levels of 5-S-cysteinyldopa are correlated to pigmentation in patients undergoing hemodialysis (2009)
  • Author(s): Murakami, K., Wakamatsu, K., Nakanishi, Y., Yamamoto, K., Kohriyama, N., Hasegawa, M., Tomita, M., Nabeshima, K., Hiki, N., Asano, S., Kawashima, S., Ito, Y., Fujita, Y., Asada, H., Nakai, S., Sugiyama, S., Ito, S.
  • Organizer: World Congress of Nephrology 2009
  • Place of Presentation: Milano, Italy
  • Year and Date: 2009-05-24
• [Book] Structure of Melanins : Melanins and Melanosomes : J. BorovanskST, P. A. Riley(eds) (2011)
  • Author(s): Ito, S., Wakamatsu, K., M. d' Ischia, A. Napolitano, A. Pezzella.
• [Book] Structure of Melanins : Melanins and Melanosomes (2011)
  • Author(s): Ito, S, Wakamatsu, K(共著)
  • Total Pages: 407
  • Publisher: Wiley-Blackwell