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Optical analysis of functional development of the spinal cord neural circuits

Research Project

Project/Area Number 21500368
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionKomazawa Women's University

Principal Investigator

SATO Katsushige  駒沢女子大学, 人間健康学部, 教授 (80291342)

Co-Investigator(Renkei-kenkyūsha) SATO Yoko (SATO MOMOSE Yoko)  関東学院大学, 人間環境学部, 教授 (70251501)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords光学イメージング / 膜電位感受性色素 / 中枢神経系 / 機能発生 / embryo / 脊髄 / 光学計測 / 鶏胚 / 生体染色 / 脊髄神経
Research Abstract

Spontaneous correlated neuronal activity during early development spreads like a wave by recruiting a large number of neurons and is considered to play a fundamental role in neural development. One important and yet unresolved question is where the activity originates, especially at the earliest stage of wave expression. In other words, which part of the brain differentiates first as a source of the correlated activity, and how does it change as development proceeds. We assessed this issue by examining the spatiotemporal patterns of the depolarization wave, the optically-identified primordial correlated activity, using the optical imaging technique with voltage-sensitive dyes. We surveyed the region responsible for the induction of the evoked and spontaneous depolarization waves in chick embryos and traced its developmental changes. The results showed that the wave initially originated in a restricted area near the obex and was generated by multiple regions at later stages. We suggest that the upper cervical cord/lower medulla near the obex is the kernel which differentiates first as the source of the correlated activity, and that regional and temporal differences in neuronal excitability might underlie the developmental profile of wave generation in early chick embryos.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (63 results)

All 2012 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (37 results) Book (7 results) Remarks (3 results)

  • [Journal Article] Optical analysis of developmental changes in synaptic potentiation in the neonatal rat corticostriatal projection2012

    • Author(s)
      Mullah, S. H.-E.-R., Inaji, M., Nariai, T., Momose-Sato, Y., Sato, K. and Ohno, K
    • Journal Title

      Neuroscience

      Volume: 201 Pages: 338-348

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization wave in the mouse embryo : origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging2012

    • Author(s)
      Momose-Sato, Y., et al
    • Journal Title

      European Journal of Neuroscience

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological mechanisms underlying the switching from the large-scale depolarization wave to segregated activity in the mouse CNS2012

    • Author(s)
      Momose-Sato, Y., et al
    • Journal Title

      European Journal of Neuroscience

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Voltage-sensitive dye imaging analysis of functional development of the neonatal rat cortocostriatal projection2011

    • Author(s)
      Inaji, M., Sato, K., Momose-Sato, Y. and Ohno, K
    • Journal Title

      NeuroImage

      Volume: 54 Pages: 1831-1839

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The embryonic brain and development of vagal pathways2011

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Journal Title

      Respiratory Physiology and Neurobiology

      Volume: 178 Pages: 163-173

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functional development of the vagal and glossopharyngeal nerve-related nuclei in the embryonic rat brainstem : Optical mapping with a voltage-sensitive dye2011

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Journal Title

      Neuroscience

      Volume: 192 Pages: 781-792

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Voltage-sensitive dye imaging analysis of functional development of the neonatal rat cortocostriatal projection.2011

    • Author(s)
      Inaji, M., et al.
    • Journal Title

      NeuroImage

      Volume: 54 Pages: 1831-1839

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The embryonic brain and development of vagal pathways.2011

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Journal Title

      Respiratory Physiology and Neurobiology

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of a neural circuit critical for imprinting behavior in chicks2010

    • Author(s)
      Nakamori, T., Sato, K., Atoji, Y., Kanamatsu T., Tanaka, K. and Ohki-Hamazaki, H
    • Journal Title

      Journal of Neuroscience

      Volume: 30 Pages: 4467-4480

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of a neural circuit critical for imprinting behavior in chicks.2010

    • Author(s)
      Nakamori, T., et al.
    • Journal Title

      Journal of Neuroscience

      Volume: 30 Pages: 4467-4480

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of a neural circuit critical for imprinting behavior in chicks2010

    • Author(s)
      Nakamori, T., et al.
    • Journal Title

      Journal of Neuroscience (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo2009

    • Author(s)
      Mochida, H., Sato, K. and Momose-Sato, Y
    • Journal Title

      European Journal of Neuroscience

      Volume: 29 Pages: 14-30

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Origin of the earliest correlated neuronal activity in the chick embryorevealed by optical imaging with voltage-sensitive dyes2009

    • Author(s)
      Momose-Sato, Y., et al.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 1-13

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Switching of the transmitters that mediate hindbrain correlated activity in the chick embryo2009

    • Author(s)
      Mochida, H., et al.
    • Journal Title

      European Journal of Neuroscience 29

      Pages: 14-30

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization wave in the mouse embryo : origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Journal Title

      European Journal of Neuroscience

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological mechanisms underlying the switching from the large-scale depolarization wave to segregated activity in the mouse CNS

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Journal Title

      European Journal of Neuroscience

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] Pharmacological nature of the depolarization wave in the mouse CNS-Dual switching and its developmental significance-2012

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Optical survey of polysynaptic pathway formation in the embryonic rat vagal system2012

    • Author(s)
      Sato, K., Nakamori, T. and Momose-Sato, Y
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス中枢神経系における広範囲脱分極波の薬理学的特性-スイッチングとその意義-2012

    • Author(s)
      佐藤容子、中森智啓、佐藤勝重
    • Organizer
      第89回日本生理学会
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report
  • [Presentation] 胎生期ラット迷走神経回路網の光学的探索2012

    • Author(s)
      佐藤勝重、中森智啓、佐藤容子
    • Organizer
      第89回日本生理学会
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dual switching in the pharmacological nature of the large-scale depolarization wave in the mouse embryo2011

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Organizer
      Society for Neuroscience 41st Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Spontaneous depolarization wave in the mouse embryo : spatial segregation with development and its mechanism2011

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Screening of fluorescent voltagesensitive dyes for detecting neural activity in the embryonic brain2011

    • Author(s)
      Sato, K., Komuro, R. and Momose-Sato, Y
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous depolarization wave in the embryonic mouse CNS : disappearance of the wave with development and its mechanism2011

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Screening of voltage-sensitive fluorescence dyes for imaging embryonic neural activity2011

    • Author(s)
      Sato, K., Komuro, R., Nakamori, T. and Momose-Sato, Y
    • Related Report
      2011 Final Research Report
  • [Presentation] NR2B-dependent neural activation is indispensable for visual imprinting2011

    • Author(s)
      Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
    • Related Report
      2011 Final Research Report
  • [Presentation] 胎生期マウス中枢神経系における自発性脱分極波の消失メカニズム2011

    • Author(s)
      佐藤容子、佐藤勝重
    • Organizer
      第88回日本生理学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 発生期中枢神経系の神経活動記録のための蛍光膜電位感受性色素のスクリーニング2011

    • Author(s)
      佐藤勝重、小室涼、佐藤容子
    • Organizer
      第88回日本生理学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 胎生期マウス中枢神経系における自発性脱分極波の消失メカニズム2011

    • Author(s)
      佐藤容子、中森智啓、佐藤勝重
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 初期胚中枢神経活動のイメージングに適する蛍光膜電位感受性色素のスクリーニング2011

    • Author(s)
      佐藤勝重、小室涼、中森智啓、佐藤容子
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 視覚的刷り込みの成立に必要なNR2B依存的な神経賦活化2011

    • Author(s)
      中森智啓、佐藤勝重、田中光一、浜崎浩子
    • Organizer
      第34回日本神経科学学会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional distribution of the palm sensory area using intraoperative intrinsic optical imaging2010

    • Author(s)
      Maehara, T., Inaji, M., Nariai, T., Sato, K. and Ohno, K
    • Organizer
      29th International Congress of Clinical Neurophysiology
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Developmental changes in the spontaneous depolarization wave in the mouse embryo : mechanisms underlying spatial segregation2010

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Organizer
      Society for Neuroscience 40th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Large-scale rhythmic depolarization wave in the embryonic avian and mammalian neural circuits2010

    • Author(s)
      Sato, K. and Momose-Sato, Y
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous depolarization waves in the developing mouse CNS : optical imaging with a voltage-sensitive dye2010

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Voltage-sensitive dye analysis of spontaneous depolarization waves in the developing mouse CNS2010

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Developmental changes in the spontaneous depolarization wave in the mouse embryo : mechanisms underlying spatial segregation.2010

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Organizer
      Society for Neuroscience 40th Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional distribution of the palm sensory area using intraoperative intrinsic optical imaging.2010

    • Author(s)
      Maehara, T., et al.
    • Organizer
      29th International Congress of Clinical Neurophysiology
    • Place of Presentation
      Kobe, Japan
    • Related Report
      2010 Annual Research Report
  • [Presentation] 発生期マウス中枢神経系における自発性広範囲脱分極波の光学的イメージング2010

    • Author(s)
      佐藤容子、佐藤勝重
    • Organizer
      第87回日本生理学会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胎生期中枢神経回路に発現する広範囲律動性脱分極波2010

    • Author(s)
      佐藤勝重、佐藤容子
    • Organizer
      第87回日本生理学会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] 発生期マウス中枢神経系における自発性広範囲伝播脱分極波の光学的解析2010

    • Author(s)
      佐藤容子、佐藤勝重
    • Organizer
      第33回日本神経科学学会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging2009

    • Author(s)
      Sato, K., Glover, J. C. and Momose-Sato, Y
    • Organizer
      XXXVI International Congress of Physiological Sciences
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Optical imaging of large-scale depolarization waves in the developing CNS2009

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Organizer
      XXXVI International Congress of Physiological Sciences
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] A telencephalic neural circuit critical for visual imprinting in chicks2009

    • Author(s)
      Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki H
    • Organizer
      XXXVI International Congress of Physiological Sciences
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous depolarization waves in the mouse embryo revealed by optical imaging with a voltage-sensitive dye2009

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Organizer
      Society for Neuroscience 39th Annual Meeting
    • Place of Presentation
      Chicago, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional development of auditory and vestibular neural circuits in the embryonic chick brainstem revealed by voltage-sensitive dye imaging2009

    • Author(s)
      Sato, K., Glover, J. C. and Momose-Sato, Y
    • Related Report
      2011 Final Research Report
  • [Presentation] Optical imaging of large-scale depolarization waves in the developing CNS2009

    • Author(s)
      Momose-Sato, Y. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] A telencephalic neural circuit critical for visual imprinting in chicks2009

    • Author(s)
      Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional development of the N. IX and N. X-related nuclei in the embryonic rat brainstem : Optical mapping with voltage-sensitive dyes2009

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Related Report
      2011 Final Research Report
  • [Presentation] Switching of the transmitters that mediate the depolarization wave in the embryonic chick CNS2009

    • Author(s)
      Momose-Sato, Y., Mochida, H. and Sato, K
    • Related Report
      2011 Final Research Report
  • [Presentation] Activation of a telencephalic circuit is critical for visual imprinting in chicks2009

    • Author(s)
      Nakamori, T., Sato, K., Tanaka, K. and Ohki-Hamazaki, H
    • Related Report
      2011 Final Research Report
  • [Presentation] Spontaneous depolarization waves in the mouse embryo revealed by optical imaging with a voltage-sensitive dye2009

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Organizer
      Society for Neuroscience 39th Annual Meeting
    • Place of Presentation
      Chicago
    • Related Report
      2009 Annual Research Report
  • [Presentation] Spontaneous depolarization wave revealed by voltage-sensitive dyeimaging2009

    • Author(s)
      Sato, K., Momose-Sato, Y.
    • Organizer
      3rd Viktor Hamburger Symposium on Motor System Development
    • Place of Presentation
      Chicago
    • Related Report
      2009 Annual Research Report
  • [Book] Comprehensive Developmental Neuroscience2012

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Publisher
      Elsevier(in press)
    • Related Report
      2011 Annual Research Report
  • [Book] Membrane Potential Imaging in the Nervous System : Methods and Applications2011

    • Author(s)
      Momose-Sato, Y., et al
    • Total Pages
      14
    • Publisher
      Springer-Verlag
    • Related Report
      2011 Annual Research Report
  • [Book] Membrane Potential Imaging in the Nervous System : Methods and Applications.2011

    • Author(s)
      Momose-Sato, Y., et al.
    • Total Pages
      14
    • Publisher
      Springer-Verlag
    • Related Report
      2010 Annual Research Report
  • [Book] Comprehensive Developmental Neuroscience.2011

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Publisher
      Elsevier(in press)
    • Related Report
      2010 Annual Research Report
  • [Book] Monitoring population membrane potential signals during functional development of neuronal circuits in vertebrate embryos. In : Membrane Potential Imaging in the Nervous System : Methods and Applications2010

    • Author(s)
      Momose-Sato, Y., Sato, K. and Kamino, K.
    • Publisher
      Springer-Verlag, New York
    • Related Report
      2011 Final Research Report
  • [Book] The Voltage Imaging Book2010

    • Author(s)
      Momose-Sato, Y., et al.
    • Publisher
      Springer, USA(In press)
    • Related Report
      2009 Annual Research Report
  • [Book] Spreading synchronized activity in the embryonic brainstem and spinal cord. In : Comprehensive Developmental Neuroscience

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Publisher
      Elsevier, USA(in press)
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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