Project/Area Number |
21500374
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neurophysiology and muscle physiology
|
Research Institution | Jikei University School of Medicine |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 前脳基底核 / シナプス / ドーパミン受容体 / カルシウムチャネル / パッチクランプ / カーボンナノチューブ / スライス / 生後発達変化 / ドーパミン / 伝達物質遊離 / ラット / 中脳 |
Research Abstract |
The present electrophysiological study has clarified a developmental increase in dopamine D1-like receptor-mediated inhibition of glutamatergic transmission onto cholinergic neurons in the rat basal forebrain through adenylyl cyclase and P/Q-type calcium channel regulation. The present electrochemical study, introducing a new biosensor material, carbon nanotube, has succeeded in the detection of physiologically released dopamine as a redox current in brain slice preparation. Furthermore, the present study has established a method for simultaneous recordings of dopamine release and membrane potential, thereby clarifying that activation of D2-like receptors reduces dopamine release by hyperpolarizing the membrane of dopaminergic neurons.
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