Functional analysis of SR protein, Mitsugumin 23.

• Project/Area Number: 21500382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kyoto University
• Principal Investigator: NISHI Miyuki 京都大学, 大学院・薬学研究科, 研究員 (60183894)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: カルシウム / 筋小胞体 / ミツグミン23

Research Abstract

Mitsugumin 23(MG23) is a 23 kDa transmembrane protein localized to the sarcoplasmic/endoplasmic reticulum and nuclear membranes in a wide variety of cells. Single-particle three-dimensional reconstruction revealed that MG23 forms a large bowl-shaped complex equipped with a putative central core. After reconstitution into planar phospholipid bilayers, purified MG23 behaved as a voltage-dependent cation-conducting channel.

Research Products

• [Journal Article] Endogenously determined restriction of food intake overcomes excitation-contraction uncoupling in JP45KO mice with aging (2012)
  • Author(s): Delbono, O., Messi M. L., Wang, Z., Treves, S., Mosca, B., Bergamelli, L., Nishi, M., Takeshima, H., Shi H., Xue B. & Zorzato, F
  • Journal Title: Exp. Gerontol Volume: 4 Issue: 4 Pages: 304-316
  • DOI: 10.1016/j.exger.2012.01.004
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms a massive bowl shaped assembly and cation-conducting channel (2011)
  • Author(s): Elisa Venruti, Kazuhiro Mio, Miyuki Nishi, Toshihiko Ogura, Toshio Moriya, Samantha Pitt, Kazutaka Okuda, Sho Kakizawa, Rebecca Sitsapesan, Chikara Sato and Hiroshi Takeshima
  • Journal Title: Biochemistry Volume: Vol.50 Issue: 13 Pages: 2623-2632
  • DOI: 10.1021/bi1019447
  • Peer Reviewed
• [Journal Article] TRIC-A channels in vascular smooth muscle contribute to blood pressure maintenance (2011)
  • Author(s): Yamazaki D, Tabara Y, Kita S, Hanada H, Komazaki S, Naitou D, Mishima A, Nishi M, Yamamura H, Yamamoto S, Kakizawa S, Miyachi H, Yamamoto S, Miyata T, Kawano Y, Kamide K, Ogihara T, Hata A, Umemura S, Soma M, Takahashi N, Imaizumi Y, Miki T, Iwamoto T, Ta
  • Journal Title: Cell Metab Volume: 14 Issue: 2 Pages: 231-241
  • DOI: 10.1016/j.cmet.2011.05.011
  • NAID: 120003255678
  • URL: http://hdl.handle.net/2433/143634
  • Peer Reviewed
• [Journal Article] Mitsugumin 23 forms a massive bowl-shaped assembly and cation-Conducting channel. (2011)
  • Author(s): Venturi E, 他10名
  • Journal Title: Biochemistry Volume: (In press)
  • Peer Reviewed
• [Journal Article] two ion-channels, TRIC-A and TRIC-B, masquerade as a single K+-channel (2010)
  • Author(s): Pitt, S. J., Park, KH, Nishi M., Urashima, T., Aoki, S., Yamazaki, D., Ma, J., Takeshima, H. & Sitsapesan R. Charade of the SR K+-channel
  • Journal Title: Biophys. J. Volume: 99 Issue: 2 Pages: 417-426
  • DOI: 10.1016/j.bpj.2010.04.051
  • Peer Reviewed
• [Journal Article] Ca2+ overload and sarcoplasmic reticulum instability in tric-a null skeletal muscle (2010)
  • Author(s): Zhao, X., Yamazaki, D., Park, KH., Komazaki, S., Tjondrokoesoemo, A., Nishi, M., Lin, P., Hirata, Y., Brotto, M., Takeshima, H & Ma, J.
  • Journal Title: J. Biol. Chem Volume: 285 Issue: 48 Pages: 37370-37376
  • DOI: 10.1074/jbc.m110.170084
  • Peer Reviewed
• [Journal Article] Facilitation of DNA damage-induced apoptosis by endplasmic reticulum protein mitsugumin23 (2010)
  • Author(s): Yamazaki, T., Sasaki, N., Nishi, M. & Takeshima, H.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 392 Issue: 2 Pages: 196-200
  • DOI: 10.1016/j.bbrc.2010.01.013
  • Peer Reviewed
• [Journal Article] MG53 constitutes a primary determinant of cardiac ischemic preconditioning (2010)
  • Author(s): Cao, CM., Zhang, Y., Weisleder, N., Ferrante, C., Wang, X., Lv, F., Zhang, Y., Song, R., Hwang, M., Jin, L., Guo, J., Peng, W., Li, G., Nishi, M., Takeshima, H., Ma, J. & Xiao, RP
  • Journal Title: Circulation Volume: 121 Issue: 23 Pages: 2565-2574
  • DOI: 10.1161/circulationaha.110.954628
  • Peer Reviewed
• [Journal Article] Ca2+ overload and sarcoplasmic reticulum instability in tric-a null Skeletal muscle. (2010)
  • Author(s): Zao X., 他10名
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 37370-37376
  • Peer Reviewed
• [Journal Article] Charade of the SR K+-channel^* two ion-channels, TRIC-A and TR, IC-B, Masquerade as asingle K+-channel. (2010)
  • Author(s): Pitt SJ, 他8名
  • Journal Title: Biophys.J. Volume: 121 Pages: 417-426
  • Peer Reviewed
• [Journal Article] Facilitation of DNA damage-induced apoptpsis by endoplasmic reticulum protein mitsugumin23 (2010)
  • Author(s): Yamazaki T., 他3名
  • Journal Title: Biochem. Biophys. Res. Cooun. 392 Pages: 196-200
  • Peer Reviewed
• [Journal Article] MG53 is an essential component of the acute membrane repair machinery (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Matsuda, N., Nishi, M., Hwang, M., Ko, JK., Lin, P., Thornton, A., Zhao, X., Pan, Z., Komazaki, S., Brotto, M., Takeshima, H & Ma, J.
  • Journal Title: Nature cell biol Volume: 11 Issue: 1 Pages: 56-64
  • DOI: 10.1038/ncb1812
  • Peer Reviewed
• [Journal Article] MG53 regulates membrane budding in muscle cells. (2009)
  • Author(s): Cai, C., Masumiya, H., Weisleder, N., Pan, Z., Nishi, M., Komazaki, S., Takeshima, H & Ma, J.
  • Journal Title: J. Biol. Chem Volume: 284 Issue: 5 Pages: 3314-3322
  • DOI: 10.1074/jbc.m808866200
  • Peer Reviewed
• [Journal Article] Membrane repair defects muscular dystrophy are linked to alterd interaction between MG53, caveolin-3 and dysferlin (2009)
  • Author(s): Cai, C., Weisleder, N., Ko, JK., Komazaki, S., Sunada, Y., Nishi, M., Takeshima, H. & Ma, J
  • Journal Title: J. Biol. Chem Volume: 284 Issue: 23 Pages: 15894-15902
  • DOI: 10.1074/jbc.m109.009589
  • Peer Reviewed
• [Journal Article] Essential role of the TRIC-B channel in Ca2+ handling of alveolar epithelial cells and inperinatal lung maturation (2009)
  • Author(s): Yamazaki, D., Komazaki, S., Nakanishi, H., Mishima, A., Nishi, M., Yazawa, M., Yamazaki, T., Taguchi, R & Takeshima H.
  • Journal Title: Development Volume: 136 Issue: 14 Pages: 2355-2361
  • DOI: 10.1242/dev.036798
  • Peer Reviewed
• [Journal Article] Essential role of the TRIC-B channel in Ca2+ handling of alveolar epithelial cells and in perinatal lung meturation (2009)
  • Author(s): Yamazaki D., 他8名
  • Journal Title: Development 136 Pages: 2355-2361
  • Peer Reviewed
• [Journal Article] Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin (2009)
  • Author(s): Cai C., 他7名
  • Journal Title: J. Biol. Chem. 284 Pages: 15894-15902
  • Peer Reviewed
• [Presentation] Upregulation of calcium influx via Cavl.1 in skeletal muscle fibers from JP45 calsequestrin 1 double knock out mice (2012)
  • Author(s): Zorsat F.…Nishi M., 他5名
  • Organizer: Biophysical Society 56th annual meeting
  • Place of Presentation: サンディエゴ(アメリカ)
  • Year and Date: 2012-02-26
• [Presentation] Up regulation of calcium influx via Cav11 in skeletal muscle fibers from JP-45 calsequestrin 1 double knock out mice (2012)
  • Author(s): Barbara Mosca, Leda Bergamelli, Mirko Vukcevic, Susan Treves, Cecilia Paoloni, Miyuki Nishi, Hiroshi Takeshima, Feliciano Protasi & Francesco Zorzato
  • Organizer: Biophysical Society 56^<th> Annual meeting
  • Place of Presentation: San Diego, California(USA)
• [Presentation] Mitsugumin 23, a protein associated with intracellular Ca2+ stores, Behaves as an ion-channel that can conduct Ca2+. (2011)
  • Author(s): Venturi E., 他10名
  • Organizer: Biophysical Society 55^<th> annual Meeting
  • Place of Presentation: Baltimore, Maryland, USA
  • Year and Date: 2011-03-07
• [Presentation] Rebbeca Sitsapesan, Chikara sato & Hiroshi Takeshima. Mitsugumin23, a protein associated with intracellular calcium stores, behaves as an ion-channel that can conduct calcium (2011)
  • Author(s): Kazuhiro Mio, Miyuki Nishi, Toshihiko Ogura, Toshio Moriya, Samantha J. Pitt, Kazutaka Okuda
  • Organizer: Biophysical Society 55^<th> Annual meeting
  • Place of Presentation: Baltimore, Maryland(USA)
• [Presentation] Role of TRIC-A channel in circulatory function (2010)
  • Author(s): Yamazaki D., 他7名
  • Organizer: Biophysical Society 54th annual Meeting
  • Place of Presentation: アメリカ
  • Year and Date: 2010-02-24
• [Presentation] Aya Mishima, Miyuki Nishi, Takahiro Iwamoto & Hiroshi Takeshima. Role of TRIC-A channel in circulatory function (2010)
  • Author(s): Daiju Yamazaki, Satomi Kota, Shinji Komazaki
  • Organizer: Biophysical Society 54^<th> Annual meeting
  • Place of Presentation: San Francisco, California(USA)
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/
• [Remarks]
  • URL: http://www.pharm.kyoto-u.ac.jp/biochem/