| Project/Area Number |
21500383
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUREBAYASHI Nagomi 順天堂大学, 医学部, 准教授 (50133335)
MORONE Nobuhiro 京都大学, 物質・細胞システム拠点, 講師 (50399680)
TAKAHAGI Takayuki 広島大学, 工学研究科, 教授 (40271069)
UEHARA Akira 福岡大学, 医学部, 准教授 (60140745)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | リアノジン受容体 / 筋小胞体 / カルシウムチャネル / 相互作用 / 悪性高熱 / 小胞体 |
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| Research Abstract |
Disease-associated mutations in ryanodine receptor/Ca^<2+> release channel hyperactivate the channel. Interdomain interaction is proposed to be involved in the mechanism of hyperactivation. Here, we examined interaction between subdomains 1 and 2 by nanogold labeling and FRET. We found that subdomains 1 and 2 are closely located and interact to each other. In addition, mutations in subdomain 3 may affect the channel gating by altering the interaction via the S4-S5 linker.
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