Identification of tyrosine-sulfated proteins essential for sperm-egg interaction

• Project/Area Number: 21500385
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Hokkaido University
• Principal Investigator: SASAKI Nobuya 北海道大学, 大学院・獣医学研究科, 准教授 (20302614)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: チロシン硫酸化 / 精巣-卵子融合 / 精巣-卵子融

Research Abstract

Tyrosine (Tyr) sulfation is a common posttranslational modification of secreted proteins or membrane-bound proteins that is implicated in numerous physiological and pathological processes. Interestingly, the Tpst2mutant shows severe hypothyroidism and male infertility due to the abnormality of sperm transport and capacitation and /or fertilization. These results suggest that the unknown testis specific protein(s) are preferentially sulfated by TPST2. In this study, I tried to identify Tyr-sulfated-protein(s) essential for sperm-egg adhesion.

Research Products

• [Journal Article] Identifying quantitative trait loci affecting resistance to congenital hypothyroidism in 129/SvJcl strain mice (2012)
  • Author(s): 細田弥生、佐々木宣哉、安居院高志
  • Journal Title: PLoS One Volume: Vol.7
  • Peer Reviewed
• [Journal Article] Current status and future prospects for research on tyrosine sulfation (2012)
  • Author(s): 佐々木宣哉
  • Journal Title: Curr Pharm Biotechnol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identifying quantitative trait loci affecting resistance to congenital hypothyroidism in 129/SvJcl strain mice (2012)
  • Author(s): 細田弥生、佐々木宣哉、亀田弥生、鳥越大輔、安居院高志
  • Journal Title: PLoS One Volume: 7巻 Issue: 1 Pages: e31035-e31035
  • DOI: 10.1371/journal.pone.0031035
  • Peer Reviewed
• [Journal Article] Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background (2010)
  • Author(s): 細田弥生、佐々木宣哉、安居院高志
  • Journal Title: Biomedical Research Volume: Vol.31 Pages: 207-211
  • NAID: 130004903803
  • Peer Reviewed
• [Journal Article] Hypothyroid phenotype of the Tpst2 mutant mouse is dependent upon genetic background. (2010)
  • Author(s): 細田弥生、佐々木宣哉、安居院高志
  • Journal Title: Biomedical Research Volume: 31卷 Pages: 207-211
  • NAID: 130004903803
  • Peer Reviewed
• [Journal Article] Current status and future prospects for research on tyrosine sulfation
  • Author(s): 佐々木宣哉
  • Journal Title: Curr Pharm Biotechnol Volume: (印刷中)
  • Peer Reviewed
• [Presentation] 129/Sv系統における甲状腺機能低下症に対する抵抗性遺伝子座の解明 (2009)
  • Author(s): 細田弥生
  • Organizer: 148回日本獣医学会学術集会
  • Place of Presentation: とりぎん文化会館(鳥取市)
  • Year and Date: 2009-09-27
• [Presentation] 129/Sv系統における甲状腺機能低下症に対する抵抗性遺伝子座の解明 (2009)
  • Author(s): 細田弥生、佐々木宣哉、安居院高志
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取市
  • Year and Date: 2009-09-25
• [Presentation] 129/Sv系統においてgrt変異による甲状腺機能低下症に抵抗性をもたらす遺伝子座の解明 (2009)
  • Author(s): 細田弥生
  • Organizer: 第6回北海道実験動物研究会
  • Place of Presentation: とかちプラザ(帯広市)
  • Year and Date: 2009-07-11
• [Presentation] 129/Sv系統における甲状腺機能低下症に対する抵抗性遺伝子座の解明 (2009)
  • Author(s): 細田弥生、佐々木宣哉、安居院高志
  • Organizer: 第6回北海道実験動物研究会
  • Place of Presentation: 帯広市
  • Year and Date: 2009-07-09