Analysis of p53 function in HTLV-1 Tax transgenic mice

• Project/Area Number: 21500390
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Kumamoto University
• Principal Investigator: OHSUGI Takeo 熊本大学, 生命資源研究・支援センター, 准教授 (00211102)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 疾患モデル / 成人T細胞白血病 / HTLV-1 / p53 / 遺伝子改変マウス / tax遺伝子 / ATL / マウス

Research Abstract

The status of p53 function before the onset of leukemia in Tax-expressing transgenic(Tg) mice was investigated. The decreased p53 transcriptional activity in Tax-Tg mice was a tissue-specific phenomenon related to Tax expression. The decline in p53 function resulted from decreased p53 accumulation after DNA damage. These results suggest that the functional inactivation of p53 by Tax may be critical for the initial onset of leukemia associated with Tax.

Research Products

• [Journal Article] Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type 1 Tax transgenic mice (2011)
  • Author(s): Ohsugi, T. and Kumasaka, T.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Altered CD4^+/CD8^+T-cell ratio in splenocytes of human T-cell leukemia virus type I Tax transgenic mice with inflammatory arthropathy (2011)
  • Author(s): Ohsugi, T. and Kumasaka, T.
  • Journal Title: Retrovirology Volume: 8
• [Journal Article] Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type 1 Tax transgenic mice (2011)
  • Author(s): Ohsugi, T., Kumasaka, T.
  • Journal Title: PLoS ONE Volume: 6 Issue: 4 Pages: e18518-e18518
  • DOI: 10.1371/journal.pone.0018518
  • Peer Reviewed
• [Journal Article] Altered CD4^+/CD8^+ T-cell ratio in splenocytes of human T-cell leukemia virus type I Tax transgenic mice with inflammatory arthropathy (2011)
  • Author(s): Ohsugi, T., Kumasaka, T.
  • Journal Title: Retrovirology Volume: 8(Suppl 1) Issue: S1
  • DOI: 10.1186/1742-4690-8-s1-a2
• [Journal Article] Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type I Tax transgenic mice. (2011)
  • Author(s): Ohsugi, T., Kumasaka, T.
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] Increased production of viral proteins by a 3'-LTR-deleted infectious clone of human T-cell leukemia virus type 1 (2009)
  • Author(s): Ohsugi, T.
  • Journal Title: Virology Journal Volume: 6 Pages: 229-229
  • Peer Reviewed
• [Journal Article] Development of Mouse Models of Adult T-Cell Leukemia for Evaluation of New Anticancer Agents (2009)
  • Author(s): Ohsugi, T. and M. Jerald Mahesh Kumar
  • Journal Title: Current Topics in Biochemical Research Volume: 11 Pages: 57-63
  • Peer Reviewed
• [Journal Article] Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-kappaB pathway (2009)
  • Author(s): Takahashi-Makise N., Suzu, S., Hiyoshi, M., Ohsugi, T., Katano, H., Umezawa, K. and Okada, S.
  • Journal Title: Int J Cancer Volume: 125 Pages: 1464-1472
  • Peer Reviewed
• [Journal Article] Biscoclaurine alkaloid cepharanthine inhibits the growth of primary effusion lymphoma in vitro and in vivo and induces apoptosis via suppression of the NF-kappaB pathway (2009)
  • Author(s): Takahashi-Makise N., Suzu, S., Hiyoshi, M., Ohsugi, T., Katano, H., Umezawa, K., Okada, S.
  • Journal Title: Int J Cancer 125 Pages: 1464-1472
  • Peer Reviewed
• [Journal Article] Increased production of viral proteins by a 3'-LTR-deleted infectious clone of human T-cell leukemia virus type 1 (2009)
  • Author(s): Ohsugi, T.
  • Journal Title: Virology Journal 6 Pages: 229-229
  • Peer Reviewed
• [Journal Article] Development of Mouse Models of Adult T-Cell Leukemia for Evaluation of New Anticancer Agents
  • Author(s): Ohsugi, T., Kumar, J.M.
  • Journal Title: Current Topics in Biomedical Research (in press)
  • Peer Reviewed
• [Presentation] Tax遺伝子導入マウスにみられる多様な疾患 (2011)
  • Author(s): 大杉剛生、島崎達也、岡田誠治、石田尚臣、熊坂利夫
  • Organizer: 第4回HTLV-1研究会
  • Place of Presentation: 東京大学(東京)
• [Presentation] Altered CD4+/CD8+T-cell ratio in splenocytes of human T-cell leukemia virus type 1 Tax transgenic mice with inflammatory arthropathy (2011)
  • Author(s): Takeo Ohsugi, Toshio Kumasaka
  • Organizer: 15th International Conference on Human Retrovirology : HTLV and Related Retroviruses
  • Place of Presentation: Pieter De Somer Aula (Leuven) Belgium
• [Presentation] Altered CD4+/CD8+ T-cell ratio in splenocytes of human T-cell leukemia virus type 1 Tax transgenic mice with inflammatory arthropathy (2011)
  • Author(s): Takeo Ohsugi, Toshio Kumasaka
  • Organizer: 15th International Conference on Human Retrovirology
  • Place of Presentation: Pieter De Somer Aula (Leuven, Belgium)
• [Presentation] Tax遺伝子導入マウスにみられる多様な疾患 (2011)
  • Author(s): 大杉剛生、島崎達也、岡田誠治、石田尚臣、熊坂利夫
  • Organizer: 第4回HTLV-1研究会
  • Place of Presentation: 東大医科研(東京都)
• [Presentation] ヒトT細胞白血病ウイルス(HTLV-1) taxトランスジェニックマウスにおける関節炎の解析 (2010)
  • Author(s): 大杉剛生、熊坂利夫
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
• [Presentation] HTLV-1 tax遺伝子導入マウスにおける関節炎とHAM/TSPとの類似性 (2010)
  • Author(s): 大杉剛生、島崎也、熊坂夫
  • Organizer: 第3回HTLV-1研究会・合同班会議
  • Place of Presentation: 東京大学(東京)
• [Presentation] エストロゲン依存性乳がんマウスモデルの樹立 (2010)
  • Author(s): Jerald Mahesh Kumar、大杉剛生
  • Organizer: 第57回日本実験動物学会総会
  • Place of Presentation: 京都テルサ(京都)
• [Presentation] HTLV-1 tax遺伝子導入マウスにおける関節炎とHAM/TSPとの類似性 (2010)
  • Author(s): 大杉剛生、島崎達也、熊坂利夫
  • Organizer: 第3回HTLV-1研究会・合同班会議
  • Place of Presentation: 東大医科研(東京都)
• [Presentation] ヒトT細胞白血病ウイルス(HTLV-1)taxトランスジェニックマウスにおける関節炎の解析. (2010)
  • Author(s): 大杉剛生、熊坂利夫
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島県徳島市)
• [Presentation] HTLV-1 Taxによるp53機能抑制機序の解析 (2009)
  • Author(s): 大杉剛生、岡田誠治、熊坂利夫
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
• [Presentation] Molecular analysis of estrogen dependent breast cancer in animal model and cell line (2009)
  • Author(s): Jerald M. Kumar, Masami Otsuka, Ken-ichi Yamamura, Yoichiro Isohama, and Takeo Ohsugi
  • Organizer: Asian Federation for Pharmaceutical Sciences 2009
  • Place of Presentation: Kyusyu University(Fukuoka)
• [Presentation] ヒトT細胞白血病ウイルス1型(HTLV-1)感染症モデルマウスのp53機能解析 (2009)
  • Author(s): 大杉剛生、鈴木操、島崎達也、岡田誠治、浦野徹、熊坂利夫
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 大宮ソニックホール(大宮)
• [Presentation] ヒトT細胞白血病ウイルス1型(HTLV-1)感染症モデルマウスのp53機能解析 (2009)
  • Author(s): 大杉剛生, 鈴木操, 島崎達也, 岡田誠治, 浦野徹, 熊坂利夫
  • Organizer: 第56回日本実験動物学会総会
  • Place of Presentation: 埼玉・大宮ソニックホール
• [Presentation] Molecular Analysis of Estrogen Dependent Breast Cancer in Animal Model and Cell Line (2009)
  • Author(s): Jerald M.Kumar, Masami Otsuka, Ken-ichi Yamamura, Yoichiro Isohama, Takeo Ohsugi
  • Organizer: Asian Federation for Pharmaceutical Sciences 2009
  • Place of Presentation: Fukuoka, Centennial Hall Kyushu University School of Medicine, Japan
• [Presentation] HTLV-1 Taxによるp53機能抑制機序の解析 (2009)
  • Author(s): 大杉剛生, 岡田誠治, 熊坂利夫
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京・都市センターホテル
• [Book] Human T-cell Leukemia Virus Type 1(HTLV-1) and Antiviral Enzyme APOBEC3 (2012)
  • Author(s): T. Ohsugi, and A. Koito
  • Publisher: NOVA Scientific publishers
• [Book] Human T-cell Leukemia Virus Type 1 (HTLV-1) and Antiviral Enzyme A POBEC3, Encyclopedia of Virology Research(Andrew P.Gatto and Benjamin S.Leon (eds)) (2012)
  • Author(s): T.Ohsugi, A.Koito
  • Publisher: NOVA Scientific publishers(印刷中)
• [Book] Molecular analysis of estrogen dependent breast cancer in animal model and cell lines. JSPS RONPAKU(Dissertation Ph. D.) program abstracts of dissertation for FY (2011)
  • Author(s): M. Jerald Mahesh Kumar and Takeo Ohsugi.
• [Book] Molecular analysis of estrogen dependent breast cancer in animal model and cell lines. JSPS RONPAKU (Dissertation Ph.D.) program abstracts of dissertation for FY 2009 (2011)
  • Author(s): M.Jerald Mahesh Kumar, Takeo Ohsugi
  • Total Pages: 88
  • Publisher: Japan Society for the Promotion of Science
• [Book] モデル動物利用マニュアル(中村卓郎編)(第2章 がん、第5節 造血系、遺伝子改変モデル、HTLV-1) (2011)
  • Author(s): 大杉剛生、山口一成
  • Publisher: 株式会社 エル・アイ・シー(印刷中)
• [Book] 九州で多発する白血病-成人T細胞白血病-『熊薬ものがたり熊本大学薬学部の研究と教育』 (2010)
  • Author(s): 大杉剛生
  • Publisher: 熊本日日新聞社
• [Book] Human T-cell Leukemia Virus Type 1(HTLV-1) and Antiviral Enzyme APOBEC3, Viral Genomes : Diversity, Properties and Parameters, Zhi Feng and Ming Long(eds) (2009)
  • Author(s): Ohsugi, T. and Koito, A.
  • Publisher: NOVA Scientific publishers
• [Book] Viral Genomes : Diversity, Properties and Parameters (2009)
  • Author(s): Ohsugi, T., Koito, A.
  • Total Pages: 267
  • Publisher: NOVA Scientific publishers
• [Book] 第2章がん、第5節造血系、遺伝子改変モデル
  • Author(s): 大杉剛生、山口一成
  • Publisher: (株)エル・アイ・シー(印刷中)
• [Book] 第2章がん、第5節造血系、遺伝子改変モデル、HTLV-1『モデル動物利用マニュアル』(中村卓郎編)
  • Author(s): 大杉剛生、山口一成
  • Publisher: 株式会社エル・アイ・シー(末定)(印刷中)
• [Remarks] 大杉剛生:遺伝子改変マウスを用いたHTLV-1感染症の解析.九州がんプロフェッショナル養成プラン(鹿児島大学)、3. 17、鹿児島市、2011.講演・セミナー
• [Remarks] Takeo Ohsugi : Transgenic mice expressing the HTLV-1 tax gene develop neoplastic and inflammatory diseases. CCMB seminar(Hyderabad, India) 5. 21, 2009.講演・セミナー
• [Remarks] Takeo Ohsugi :「Microbiological monitoring in laboratory Animals」Animal House seminal(Hyderabad, India) 5. 19, 2009.講演・セミナー