Generation of knockout mice and transgenic mice of the Alivin family

• Project/Area Number: 21500396
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: 公益財団法人東京都医学総合研究所 (2012); Tokyo Metropolitan Organization for Medical Research (2009-2011)
• Principal Investigator: ONO Tomio 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (60231239)
• Co-Investigator(Kenkyū-buntansha): SHITARA Hiroshi 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (90321885) ; MATSUOKA Kunie 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40291158) ; TAYA Choji 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 室長 (90175456)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: ノックアウトマウス / アポトーシス / 神経細胞死 / ロイシンリッチリピート / 免疫グログリン / 神経活動 / alivin / amigo

Research Abstract

In order to study biological functions of the Alivin family, we have generated mutant stains of mice harboring Alivin1 knockout (KO) allele, Alivin2 KO allele, Alivin3 KO allele, and an Alivin1 over-expressing transgene. Results of open-field tests suggest that the transgenic mice over-expressing ALIVIN1 have enhanced anxiety. The fibroblast prepared from embryos of the Alivin1 KO mice showed reduced migratory activity compared with that of wild type.

Research Products

• [Journal Article] Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging (2012)
  • Author(s): Yamaguchi J, Nishiyama S, Shimanuki M, Ono T,Sato A, Nakada K, Hayashi J, Yonekawa H, Shitara H
  • Journal Title: Transgenic Res 21 Pages: 439-47
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21792696
  • Peer Reviewed
• [Journal Article] Comprehensive application of an mtDsRed2-Tg mouse strain for mitochondrial imaging. (2012)
  • Author(s): Yamaguchi J, Nishiyama S, Shimanuki M, Ono T, Sato A, Nakada K, Hayashi J, Yonekawa H, Shitara H
  • Journal Title: Transgenic Res Volume: 21 Pages: 439-47
  • Peer Reviewed
• [Journal Article] Over-expression of Tfam improves the mitochondrial disease phenotypes in a mouse model system (2010)
  • Author(s): Nishiyama S, Shitara H, Nakada K, Ono T, Sato A, Suzuki H, Ogawa T,Masaki H, Hayashi J, Yonekawa H
  • Journal Title: Biochem Biophys Res Commun Volume: 401 (1) Pages: 26-31
  • NAID: 120007137866
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20816751
  • Peer Reviewed
• [Journal Article] Over-expression of Tfam improves the mitochondrial disease phenotypes in a mouse model system. (2010)
  • Author(s): Nishiyama S, Shitara H, Nakada K, Ono T, Sato A, Suzuki H, Ogawa T, Masaki H, Hayashi J, Yonekawa H.
  • Journal Title: Biochem Biophys Res Commun. Volume: 401(1) Pages: 26-31
  • NAID: 120007137866
  • Peer Reviewed
• [Journal Article] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Journal Title: Neurosci Res Volume: 65
• [Journal Article] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., Yonekawa H.
  • Journal Title: Neurosci Res 65
• [Presentation] 血管新生におけるCK2とポリアミン の関与 (2012)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 島村眞里子
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] A new spontaneous mouse mutant of the kyphoscoliosis peptidase gene, a muscular dystrophy gene (2011)
  • Author(s): Tomio Ono, Toyoyuki Takada, Kazuhiko Watabe, Hiroshi Shitara, Rie Ishii, Toshihiko Shiroishi , Choji Taya, and Hiromichi Yonekawa
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] CK2阻害物質は血管新生を抑制する (2011)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 島村眞里子
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] IkBキナーゼ阻害剤によるin vivo 血管新生の抑制 (2010)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 山本行男, 島村眞里子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] 1kBキナーゼ阻害剤によるin vivo血管新生の抑制 (2010)
  • Author(s): 芦野洋美, 小野富男, 入江敦, 山本行男, 島村眞里子
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Enhanced locomotor activity in alivin1/amigo2 deficient mice (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
• [Presentation] Inhibitory effect of N-acetyl-L-cysteine on angiogenesis in vivo (2009)
  • Author(s): Ashino,H., Ono,T., Yamamoto,Y., Shimamura,M
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Deficiency of alivin1/amigo2 in mice enhances locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa, H
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
• [Presentation] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., and Yonekawa H
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] Mice deficient in alivin1/amigo2 show enhanced locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., Yonekawa H.
  • Organizer: 第32回日本神経科学大会
  • Place of Presentation: 愛知県・名古屋国際会議場
• [Presentation] Deficiency of alivin1/amigo2 in mice enhances locomotor activity (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., Yonekawa, H.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸国際会議場、神戸国際展示場
• [Presentation] Inhibitory effect of N-acety1-L-cysteine on angiogenesis in vivo (2009)
  • Author(s): Ashino, H., Ono, T., Yamamoto, Y., Shimamura, M
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸国際会議場、神戸国際展示場
• [Presentation] Enhanced locomotor activity in alivin1/amigo2 deficient mice (2009)
  • Author(s): Ono T., Akamatsu N., Shitara H., Ishii R., Taya C., Yamada I, Shibukawa Y., Kushida T., Furuse T., Watabe K., Wakana S., Yonekawa H.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県・パシフィコ横浜
• [Remarks]
  • URL: http://www.rinshoken.or.jp/
• [Remarks]
  • URL: http://www.rinshoken.or.jp/LAS/