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Ovarian cryopreservation in Syrian hamsters, Mongolian gerbils, and guinea pigs

Research Project

Project/Area Number 21500398
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNational Institute of Biomedical Innovation

Principal Investigator

SUZUKI Osamu  独立行政法人医薬基盤研究所, 難病・疾患資源研究部, 主任研究員 (70235935)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords系統保存 / 卵巣凍結 / ハムスター / スナネズミ / モルモット / モルモソト
Research Abstract

Preservation of biological resources became more important, but no method for embryo/sperm cryopreservation is well established in Syrian hamsters, Mongolian gerbils, or guinea pigs, which are often used in laboratories. In this study, I tried to establish a method for preservation of these species by ovarian cryopreservation. I obtained pups by orthotopic transfers of vitrified ovaries in Syrian hamsters, but not in gerbils or guinea pigs. Graft immunity and operating procedures need to be considered for ovarian transfers in these two species.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (52 results)

All 2012 2011 2010 2009

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (46 results)

  • [Journal Article] Adult onset cardiac dilatation in a transgenic mouse line with Gal.BETA.1,3GalNAc .ALPHA.2,3-sialyltransferase II (ST3Gal-II) transgenes: a new model for dilated cardiomyopathy2011

    • Author(s)
      Suzuki O, Kanai T, Nishikawa T, Yamamoto Y, Noguchi A, Takimoto K, Koura K, Noguchi Y, Uchio-Yamada K, Tsuji S, Matsuda J
    • Journal Title

      Proceedings of the Japan Academy, Series B

      Volume: 87 Issue: 8 Pages: 550-562

    • DOI

      10.2183/pjab.87.550

    • NAID

      130001224823

    • ISSN
      0386-2208, 1349-2896
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic replacement of histone H3 variants reprograms epigenetic marks in early mouse embryos2011

    • Author(s)
      Akiyama T
    • Journal Title

      PLOS Genetics

      Volume: 7 Issue: 10 Pages: e1002279-e1002279

    • DOI

      10.1371/journal.pgen.1002279

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of sample mixtures for standard curve creation in quantitative Western blots2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Journal Title

      Exp. Anim.

      Volume: 60(2) Pages: 193-196

    • NAID

      10029550480

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of sample mixtures for standard curve creation in quantitative Western blots.2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Journal Title

      Exp.Anim.

      Volume: 60(2) Pages: 193-196

    • NAID

      10029550480

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex2009

    • Author(s)
      Okado H, Ohtaka-Maruyama C, Sugitani Y, Fukuda Y, Ishida R, Hirai S, Miwa A, Takahashi A, Aoki K, Mochida K, Suzuki O, Honda T, Nakajima K, Ogawa M, Terashima T, Matsuda J, Kawano H, Kasai M
    • Journal Title

      Dev Biol

      Volume: 331(2) Issue: 2 Pages: 140-151

    • DOI

      10.1016/j.ydbio.2009.04.030

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The transcriptional repressor RP58 is crucial for cell-division patterning and neuronal survival in the developing cortex.2009

    • Author(s)
      Okado H, Ohtaka-Maruyama C, Sugitani Y, Fukuda Y, Ishida R, Hirai S, Miwa A, Takahashi A, Aoki K, Mochida K, Suzuki O, Honda T, Nakajima K, Ogawa M, Terashima T, Matsuda J, Kawano H, Kasai M
    • Journal Title

      Dev Biol. 331(2)

      Pages: 140-151

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Effects of dehydroepiandrosterone on sperm fertilizability in vitro and testicular gene expression2012

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      38th Annual Conference of the IETS(第38回国際胚移植学会年次総会)
    • Place of Presentation
      Phoenix, Arizona, USA(フェニックス)
    • Related Report
      2011 Final Research Report
  • [Presentation] Effects of dehydroepiandrosterone on sperm fertilizability in vitro and testicular gene expression2012

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      38th Annual Conference of the IETS
    • Place of Presentation
      Phoenix, AZ
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of ovarian size on the viability of cryopreserved Syrian hamster ovaries2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, and Matsuda J
    • Organizer
      37th Annual Conference of the International Embryo Transfer Society(第37回国際胚移植学会年次総会)
    • Place of Presentation
      Orland, FL, USA(オーランド)
    • Related Report
      2011 Final Research Report
  • [Presentation] Elevated O-GlcNAcylation of ATP synthaseβsubunits in cardiomyopathic hearts in Syrian hamsters2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      Experimental Biology 2011
    • Place of Presentation
      Washington, DC, USA(ワシントンDC)
    • Related Report
      2011 Final Research Report
  • [Presentation] シアル酸転移酵素過剰発現マウスの心筋症様心臓におけるCalreticulinの増加2011

    • Author(s)
      鈴木治,小浦美奈子,野口洋子,山田-内尾こずえ,松田潤一郎
    • Organizer
      第58回日本実験動物学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Sequence analysis of the ovarian Kiss1 cDNA in the Mongolian gerbil and Syrian hamster2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      ENDO 2011 : The 93rd Annual Meeting & Expo(アメリカ内分泌学会)
    • Place of Presentation
      Boston, Massachusetts, USA(ボストン)
    • Related Report
      2011 Final Research Report
  • [Presentation] 雄へのdehydroepiandrosterone徐放剤投与がマウス体外受精へ及ぼす影響2011

    • Author(s)
      鈴木治,小浦美奈子,野口洋子,山田-内尾こずえ,松田潤一郎
    • Organizer
      第104回日本繁殖生物学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Increase of calreticulin in hearts of cardiomyopathic mice with sialyltransferase transgenes2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      2011 AALAS National Meeting
    • Place of Presentation
      サンディエゴ
    • Related Report
      2011 Final Research Report
  • [Presentation] Effects of dehydroepiandrosterone on androgen receptor protein expression in testes and epididymides in two strains of mice2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      2011 annual meeting of the American Society for Cell Biology(第51回アメリカ細胞生物学会年次総会)
    • Place of Presentation
      Denver, Colorado, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] 拡張型心筋症の動物モデル2種(マウスとハムスター)における心臓カルシニューリンの発現比較2011

    • Author(s)
      鈴木治,小浦美奈子,野口洋子,山田-内尾こずえ,松田潤一郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Elevated O-GlcNAcylation of ATP synthase β subunits in cardiomyopathic hearts in Syrian hamsters2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      Experimental Biology 2011
    • Place of Presentation
      Washington, DC
    • Related Report
      2011 Annual Research Report
  • [Presentation] Sequence analysis of the ovarian Kiss1 cDNA in the Mongolian gerbil and Syrian hamster2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      ENDO 2011 : The 93rd Annual Meeting
    • Place of Presentation
      Boston, MA
    • Related Report
      2011 Annual Research Report
  • [Presentation] Increase of calreticulin in hearts of cardiomyopathic mice with sialyltransferase transgenes2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K
    • Organizer
      2011 AALAS National Meeting
    • Place of Presentation
      San Diego, CA
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effects of dehydroepiandrosterone on androgen receptor protein expression in testes and epididymides in two strains of mice2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      2011 annual meeting of the American Society for Cell Biology
    • Place of Presentation
      Denver, CO
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of ovarian size on the viability of cryopreserved Syrian hamster ovaries.2011

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      37th Annual Conference of the International Embryo Transfer Society
    • Place of Presentation
      Wyndham Orlando Resort, Orland, FL, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improved superovulation induction in A/J mice based on the luteinizing hormone receptor protein expression pattern2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      14th International Congress of Endocrinology(第14回国際内分泌会議)
    • Place of Presentation
      Kyoto, Japan(京都)
    • Related Report
      2011 Final Research Report
  • [Presentation] Sequence analysis of type VI collagen subunits in cardiomyopathic hamster hearts2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, and Matsuda J
    • Organizer
      Experimental Biology 2010
    • Place of Presentation
      Anaheim, CA, USA(アナハイム)
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス排卵誘起におけるhCGの血中動態2010

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第57回日本実験動物学会総会
    • Place of Presentation
      京都市
    • Related Report
      2011 Final Research Report
  • [Presentation] Heart miRNA cloning in cardiomyopathic(J2N-k) and normal(J2N-n) Syrian hamsters2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, and Matsuda J
    • Organizer
      GENETICS 2010 : Model Organisms to Human Biology meeting(遺伝学2010ミーティング:モデル生物からヒト生物学へ)
    • Place of Presentation
      Boston, MA, USA(ボストン)
    • Related Report
      2011 Final Research Report
  • [Presentation] シリアンハムスター卵巣凍結保存における卵巣サイズの影響2010

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第103回日本繁殖生物学会総会
    • Place of Presentation
      十和田市
    • Related Report
      2011 Final Research Report
  • [Presentation] 急速進行性糸球体腎炎モデルSCG/ThpNkcマウスの繁殖及び腎炎の発症状況について2010

    • Author(s)
      小浦美奈子、島かおる、中村和臣、野口洋子、鈴木治、鈴木和男、亀岡洋祐、松田潤一郎
    • Organizer
      第44回日本実験動物技術者協会総会
    • Place of Presentation
      旭川
    • Related Report
      2011 Final Research Report
  • [Presentation] BALB/c凍結胚における個体復元率の改善2010

    • Author(s)
      中村和臣,小浦美奈子,野口洋子,鈴木治,松田潤一郎
    • Organizer
      第44回日本実験動物技術者協会総会
    • Place of Presentation
      旭川
    • Related Report
      2011 Final Research Report
  • [Presentation] O-GlcNAcylation of heart proteins in cardiomyopathic(J2N-k) and normal(J2N-n) Syrian hamsters2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Suzuki O, Koura M, Noguchi Y
    • Organizer
      60th National Meeting of the American Association for Laboratory Animal Science(第61回アメリカ実験動物学会年次総会)
    • Place of Presentation
      Atlanta, GA, USA(アトランタ)
    • Related Report
      2011 Final Research Report
  • [Presentation] Elevated O-GlcNAcylation of heart proteins in cardiomyopathic transgenic mice with St3gal2 transgene2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Suzuki O, Koura M, Noguchi Y
    • Organizer
      49th Annual Meeting of the American Society for Cell Biology(第50回アメリカ細胞生物学会年次総会)
    • Place of Presentation
      Philadelphia, PA, USA(フィラデルフィア)
    • Related Report
      2011 Final Research Report
  • [Presentation] Sequence analysis of type VI collagen subunits in cardiomyopathic hamster hearts.2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Organizer
      Experimental Biology 2010
    • Place of Presentation
      Anaheim convention center, Anaheim, CA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス排卵誘趣とおげるhCGの血中動態2010

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第57回日本実験動物学会総会
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Heart miRNA cloning in cardiomyopathic (J2N-k) and normal (J2N-n) Syrian hamsters.2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Organizer
      GENETICS 2010 : Model Organisms to Human Biology meeting
    • Place of Presentation
      Sheraton Boston Hotel, Boston, MA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] シリアンハムスター卵巣凍結保存における卵巣サイズの影響2010

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第103回日本繁殖生物学会総会
    • Place of Presentation
      北里大学(十和田市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 急速進行性糸球体腎炎モデルSCG/ThpNkcマウスの繁殖及び腎炎の発症状況について2010

    • Author(s)
      小浦美奈子、島かおる、中村和臣、野口洋子、鈴木治、鈴木和男、亀岡洋祐、松田潤一郎
    • Organizer
      第44回日本実験動物技術者協会総会
    • Place of Presentation
      旭川市民文化会館(旭川市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] O-GlcNAcylation of heart proteins in cardiomyopathic (J2N-k) and normal (J2N-n) Syrian hamsters.2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Organizer
      61th National Meeting of the American Association for Laboratory Animal Science
    • Place of Presentation
      Georgia World Congress Center, Atlanta, GA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Elevated O-GlcNAcylation of heart proteins in cardiomyopathic transgenic mice with St3gal2 transgene.2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      50th Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      Pennsylvania Convention Center, Philadelphia, PA, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] Improved superovulation induction in A/J mice based on the luteinizing hormone receptor protein expression pattern.2010

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      14th International Congress of Endocrinology
    • Place of Presentation
      京都国際会館(京都)
    • Related Report
      2009 Annual Research Report
  • [Presentation] クローズドコロニーddYの中から発見された走行発作を伴うてんかん様の症状を呈するマウスについて2009

    • Author(s)
      小浦美奈子、野口洋子、鈴木治、中村和臣、松田潤一郎
    • Organizer
      第104回関西実験動物研究会
    • Place of Presentation
      京都
    • Year and Date
      2009-12-11
    • Related Report
      2011 Final Research Report
  • [Presentation] クローズドコロニーddYの中から発見された走行発作を伴うてんかん様の症状を呈するマウスについて2009

    • Author(s)
      小浦美奈子、野口洋子、鈴木治、中村和臣、松田潤一郎
    • Organizer
      第104回関西実験動物研究会
    • Place of Presentation
      都市勧業館「みやこめっせ」(京都)
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] Accumulation of type VI collagen in cardiomyopathic monkey hearts2009

    • Author(s)
      Suzuki O, Ageyama N, Terao K
    • Organizer
      Experimental Biology 2009
    • Place of Presentation
      New Orleans, LA, USA(ニューオリンズ)
    • Related Report
      2011 Final Research Report
  • [Presentation] 心筋症シリアンハムスター(J2N系)の心臓における6型コラーゲンの増加2009

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎、高木博隆
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      大宮
    • Related Report
      2011 Final Research Report
  • [Presentation] 低蛋白飼料投与によるELてんかんモデルマウスの繁殖効率の改善(II)-臓器重量、血液生化学値の検討2009

    • Author(s)
      野口洋子、高野薫、小浦美奈子、中村和臣、鈴木治、松田潤一郎
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      大宮
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス性腺刺激ホルモン受容体の系統間比較2009

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第102回日本繁殖生物学会総会
    • Place of Presentation
      奈良
    • Related Report
      2011 Final Research Report
  • [Presentation] Increased collagen type VI content in the hearts of cardiomyopathic Syrian hamsters(J2N strain)2009

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      60th National Meeting of the American Association for Laboratory Animal Science(第60回アメリカ実験動物学会年次総会)
    • Place of Presentation
      Denver, CO, USA(デンバー)
    • Related Report
      2011 Final Research Report
  • [Presentation] Strain difference in luteinizing hormone receptor protein expression in mouse ovaries2009

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J
    • Organizer
      49th Annual Meeting of the American Society for Cell Biology(第49回アメリカ細胞生物学会年次総会)
    • Place of Presentation
      San Diego, CA, USA(サンディエゴ)
    • Related Report
      2011 Final Research Report
  • [Presentation] Accumulation of type VI collagen in cardiomyopathic monkey hearts2009

    • Author(s)
      Suzuki O, Ageyama N, Terao K.
    • Organizer
      Experimental Biology 2009
    • Place of Presentation
      Ernest N Morial Convention Center, New Orleans, LA, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] 心筋症シリアンハムスター(J2N系)の心臓における6型コラーゲンの増加2009

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎、高木博隆
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      大宮ソニックシティ(大宮)
    • Related Report
      2009 Annual Research Report
  • [Presentation] 低蛋白飼料投与によるELてんかんモデルマウスの繁殖効率の改善(II)-臓器重量、血液生化学値の検討2009

    • Author(s)
      野口洋子、高野薫、小浦美奈子、中村和臣、鈴木治、松田潤一郎
    • Organizer
      第56回日本実験動物学会総会
    • Place of Presentation
      大宮ソニックシティ(大宮)
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウス性腺刺激ホルモン受容体の系統間比較2009

    • Author(s)
      鈴木治、小浦美奈子、野口洋子、山田-内尾こずえ、松田潤一郎
    • Organizer
      第102回日本繁殖生物学会総会
    • Place of Presentation
      近畿大学(奈良)
    • Related Report
      2009 Annual Research Report
  • [Presentation] Increased collagen type VI content in the hearts of cardiomyopathic Syrian hamsters(J2N strain)2009

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Organizer
      60th National Meeting of the American Association for Laboratory Animal Science
    • Place of Presentation
      Colorado Convention Center, Denver, CO, USA
    • Related Report
      2009 Annual Research Report
  • [Presentation] Strain difference in luteinizing hormone receptor protein expression in mouse ovaries.2009

    • Author(s)
      Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
    • Organizer
      49th Annual Meeting of the American Society for Cell Biology
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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