Effect of mechanical stress to ligament/tendon fibroblast under 3D culture environment

• Project/Area Number: 21500399
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Hokkaido University
• Principal Investigator: MAJIMA Tokifumi 北海道大学, 大学院・医学研究科, 特任教授 (30241334)
• Co-Investigator(Kenkyū-buntansha): SAWAGUCHI Naohiro 北海道大学, 北海道大学病院, 助教 (80435982) ; KASAHARA Yasuhiko 北海道大学, 北海道大学病院, 助教 (00581927)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: バイオメカニクス / 腱・靭帯 / 線維芽細胞 / リモデリング / 繊維芽細胞

Research Abstract

Tissue engineering techniques using biodegradable three-dimensional (3D) scaffolds with cultured cells offer more potential alternatives for the treatment of severe ligament and tendon injuries. In tissue engineering, one of the crucial roles of 3D scaffolds is to provide a temporary template with the biomechanical characteristics of the native extracellular matrix (ECM) until the regenerated tissue matures. The purpose of the present study was to assess the effect of various cyclic mechanical stresses on cell proliferation and ECM production in a 3D scaffold made from chitosan and hyaluronan for ligament and tendon tissue engineering.
Multidimensional cyclic mechanical strain to mimic the physiological condition in vivo has the potential to improve or accelerate tissue regeneration in ligament and tendon tissue engineering using 3D scaffolds in vitro.

Research Products

• [Journal Article] MEffect of Japanese herbal medicine, Boiogito, on osteoarthritic knee with joint effusion (2012)
  • Author(s): Majima T, Inoue M, et. al
  • Journal Title: Sports Med, Arthroscopy, Rehabilitation, Therapy & Technology Volume: 4 Issue: 1 Pages: 3-3
  • DOI: 10.1186/1758-2555-4-3
  • Peer Reviewed
• [Journal Article] A biomechanical and histological evaluation of a tissue engineered ligament using chitosan and hyaluronan hybrid polymer fibers : A rabbit medial collateral ligament reconstruction model (2011)
  • Author(s): Irie T, , Majima T, et. al
  • Journal Title: J Biomed Mat Res A
  • Peer Reviewed
• [Journal Article] Patella eversion deteriorates early knee functional recovery after TKA -Comparison between MIS TKA and conventional TKA- (2011)
  • Author(s): Majima T, Nishiike O, et. al
  • Journal Title: Arthritis Volume: 2011 Pages: 1-6
  • DOI: 10.1155/2011/854651
  • Peer Reviewed
• [Journal Article] キトサン・ヒアルロンサ酸ハイブリッド繊維を用いた組織工学的手法による靱帯再生 (2011)
  • Author(s): 眞島任史
  • Journal Title: 整形外科 Volume: 62 Pages: 1088-1088
• [Journal Article] キトサン・ヒアルロンサ酸ハイブリッド繊維を用いた組織工学的手法による靭帯再生 (2011)
  • Author(s): 真島任史
  • Journal Title: 整形外科 Volume: 62 Pages: 1-088
  • Peer Reviewed
• [Journal Article] A biomechanical and histological evaluation of a tissue engineered ligament using chitosan and hyaluronan hybrid polymer fibers : A rabbit medial collateral ligament reconstruction model. (2011)
  • Author(s): Irie T, Maiima T, et.al.
  • Journal Title: J Biomed Mat Res A. Volume: Mar2.Doi:10100(Epub)
  • Peer Reviewed
• [Journal Article] Effect of cyclic 3D strain on cell proliferation and collagen synthesis of fibroblast seeded chitosan-hyalronan hybrid polymer fiber (2010)
  • Author(s): Sawaguchi N, Majima T, et. Al
  • Journal Title: J Orthop Sci Volume: 15 Pages: 569-577
  • Peer Reviewed
• [Journal Article] Effect of cyclic 3D strain on cell proliferation and collagen synthesis of fibroblast seeded chitosan-hyalronan hybrid polymer fiber. (2010)
  • Author(s): Sawaguchi N, Maiima T, et.al
  • Journal Title: J Orthop Sci Volume: 15 Pages: 569-577
  • Peer Reviewed
• [Presentation] Chitosan-based Hyaluronan Hybrid Polymer Fiber Scaffold for Liga ment/Tendon Tissue Engineering (2011)
  • Author(s): Majima T
  • Organizer: The 5th International Cell Therapy Conference
  • Place of Presentation: Seoul National University Hospital, Seoul
  • Year and Date: 2011-11-01
• [Presentation] Chitosan-based Hyaluronan Hybrid Polymer Fiber Scaffold for Ligament/Tendon Tissue Engineering (2011)
  • Author(s): Majima T
  • Organizer: The 5th International Cell Therapy Conference
  • Place of Presentation: Seoul National University Hospital, Seoul(招待講演)
  • Year and Date: 2011-11-01
• [Presentation] キトサン・ヒアルロン酸ハイブリット繊維を用いた組織工学的手法による靭帯再生 (2010)
  • Author(s): 眞島任史
  • Organizer: 日本整形外科基礎学会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-10-15
• [Book] The knee A comprehensisve Review, Osteotomies about the knee. Chapter 12 (2010)
  • Author(s): Majima T
  • Total Pages: 594
  • Publisher: World Scientific, New Jersey, USA
• [Book] Chapter 12, Osteotomies about the knee
  • Author(s): Majima T
  • Publisher: New Jersey, USA