| Project/Area Number |
21500403
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | University of Toyama |
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Principal Investigator |
OGAWA Ryohei 富山大学, 大学院・医学薬学研究部(医学), 講師 (60334736)
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| Co-Investigator(Kenkyū-buntansha) |
KAGIYA Go 北里大学, 医療衛生学部, 講師 (30524243)
ZHAO Qing-li 富山大学, 大学院・医学薬学研究部(医学), 助教 (90313593)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 生体制御 / 治療 / プロモーター / 遺伝子発現制御 / 遺伝子治療 / マイクロRNA / 遺伝子発現 / 遺伝子冶療 |
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| Research Abstract |
We have established a retrovirus vector system to stably introduce a gene cassette consisting of a constructed radiation responsive promoter and a gene of interest. In a cell line expressing the luciferase gene, we showed expression of the luciferase gene was possible with radiation. In addition, with an established cell line expressing the fcy :: fur gene, a suicide gene, it was possible to enhance cell killing effect when radiation was delivered, showing radiation dependent suicide gene therapy simulation in vitro. Furthermore, using another set of cis-elements, we successfully obtained radiation responsive promoters effectively working in a prostate cancer cell line. Moreover, we found that sonication changed expressions of microRNAs that could be involved in response of a cell to ultrasound. In addition, we showed that target sequences of microRNAs that decrease their expressions by sonication could be a more fine-tuned ultrasound mediated gene regulation system when combined with an ultrasound responsive promoter.
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