Creation of novel matrix for cell culture using CBMand chitin

• Project/Area Number: 21500423
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NAKAMURA Satoshi 東京工業大学, 大学院・生命理工学研究科, 教授 (50227899)
• Research Collaborator: FUKAGAWA Satoko 東京工業大学, 大学院・生命理工学研究科, 学生
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 再生医工学材料 / CBM / キチン / マトリックス / キチナーゼ / 再生医療 / フィブロブラスト / RGD配列 / PHSRN配列

Research Abstract

Chitinase J from alkaliphilic Bacillus sp. contained a catalytic domain, a fibronectin type III-like domain(FnIIID) and a chitin-binding domain(ChBD). A peptide sequence Arg-Gly-Asp(RGD) sequence locates within fibronectin, an extracellular matrix molecule, and interacts with cells via a cell surface receptor called integrin. Several kinds of RGD-fused FnIIID/ChBD and ChBD proteins were prepared and characterized. The fusion proteins indicated chitin-binding, integrin-binding and cell adhesive activities.

Research Products

• [Journal Article] Mutational analysis of a CBM family 5 chitin-binding domain of an alkaline chitinase from Bacillus sp (2012)
  • Author(s): F. Uni, S. Lee, R. Yatsunami, T. Fukui, and S. Nakamura
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 76 Pages: 530-535
  • Peer Reviewed
• [Journal Article] Mutational analysis of a CBM family 5 chitin-binding domain of an alkaline chitinase from Bacillus sp.J813 (2012)
  • Author(s): Fumiya Uni, 他
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 76 Pages: 530-535
  • Peer Reviewed
• [Journal Article] Mechanism of the thermal inactivation of a MBP-fused heparinase I : Biochemical investigations and kinetic modeling (2011)
  • Author(s): S. Chen, F. Ye, H. Zhao, Y. Chena, Y. Chen, S. Nakamura, F. Arisaka and X. Xing
  • Journal Title: Biotechnol. Bioeng Volume: 108 Pages: 1841-1851
  • Peer Reviewed
• [Journal Article] alcium-dependent xylan-binding domain of alkaline xylanase from alkaliphilic Bacillus sp. strain 41M-1 (2011)
  • Author(s): R. Yazawa, J. Takakura, T. Sakata, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura, A
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 75 Pages: 379-381
  • Peer Reviewed
• [Journal Article] Biochemical analysis and kinetic modeling of the thermal inactivation of MBP-fused heparinase I : Implications for a comprehensive thermostabilization strategy (2011)
  • Author(s): Chen S, Ye F, Chen Y, Chen Y, Zhao H, Yatsunami R, Nakamura S, Arisaka F, Xing XH.
  • Journal Title: Biotechnol Bioeng Volume: 108(8) Issue: 8 Pages: 1841-51
  • DOI: 10.1002/bit.23144
  • Peer Reviewed
• [Journal Article] A calcium-dependent xylan-binding domain of alkaline xylanase from alkaliohilic Bacillus sp. strain 41M-1 (2011)
  • Author(s): Risa Yazawa, 他
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 75(2) Pages: 379-381
  • Peer Reviewed
• [Journal Article] Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution (2010)
  • Author(s): W. Tsukimura, K. Watanabe, C. Morokuma, R. Yatsunami, T. Fukui and S. Nakamura
  • Journal Title: Jpn. Soc. Extr. Volume: 9 Pages: 15-18
  • Peer Reviewed
• [Journal Article] Gene expression and characterization of a novel GH family 18 chitinase from extremely halophilic archaeon Halobacterium salinarum NRC-1 (2010)
  • Author(s): R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, Y. Zhang, T. Takashina, T. Fukui and S. Nakamura
  • Journal Title: pn. Soc. Extr. Volume: 9 Pages: 19-24
  • Peer Reviewed
• [Journal Article] Characterization of a haloarchaeal GH family 18 chitinase with additional acidic amino acids on its protein surface (2010)
  • Author(s): Y. Zhang, R. An, R. Yatsunami, M. Sato, K. Orishimo, Y. Hatori, T. Fukui and S. Nakamura
  • Journal Title: Jpn. Soc. Extr. Volume: 9 Pages: 72-74
  • Peer Reviewed
• [Journal Article] Improvement of alkaliphily of thermostable GH family 10 xylanase from Thermotoga maritima by directed evolution (2010)
  • Author(s): Wataru Tsukimura, 他
  • Journal Title: J.Jpn.Soc.Extr. Volume: 9(1) Pages: 15-18
  • Peer Reviewed
• [Journal Article] Characterization of a haloarchaeal GH family 18 chitinase with additional acidic amino acids on its protein surface (2010)
  • Author(s): Yang Zhang, 他
  • Journal Title: J.Jpn.Soc.Extr. Volume: 9(2) Pages: 72-74
  • Peer Reviewed
• [Journal Article] Improvement of alkaliphily of Bacillus alkaline xylanase by introducing amino acid substitutions both on catalytic cleft and protein surface, Biosci (2009)
  • Author(s): H. Umemoto, Ihsanawati, M. Inami, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura
  • Journal Title: Biotechnol. Biochem. Volume: 73 Pages: 965-967
  • Peer Reviewed
• [Journal Article] Additional carbohydrate-binding modules enhance the insoluble substrate hydrolytic activity of beta-1, 3-glucanase from alkaliphilic Nocardiopsis sp. F96 (2009)
  • Author(s): N. Koizumi, S. Masuda, K. Maeda, Y. Isoda, R. Yatsunami, T. Fukui and S. Nakamura
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 73 Pages: 1078-1082
  • Peer Reviewed
• [Journal Article] Analysis of functional domains and improvement of alkaliphily of an alkaline xylanase on the basis of its three-dimensional structure (2009)
  • Author(s): H. Umemoto, R. Yazawa, J. Takakura, R. Yatsunami, T. Fukui and S. Nakamura
  • Journal Title: J. Appl. Glycosci. Volume: 57 Pages: 145-150
  • NAID: 10026168155
  • Peer Reviewed
• [Journal Article] Role of exposed aromatic residues in substrate-binding of CBM family 5 chitin-binding module of alkaline chitinase (2009)
  • Author(s): F. Uni, S. Lee, R. Yatsunami, T. Fukui and S. Nakamura
  • Journal Title: Nucleic Acids Symp. Ser. Volume: 53 Pages: 311-312
• [Journal Article] Isolation of anti-uroporphyrin antibody fragment using phage display techniques (2009)
  • Author(s): Toshiya Ohta, 他
  • Journal Title: Porphirins 18 Pages: 1-6
  • Peer Reviewed
• [Journal Article] Improvement of alkaliphily of Bacillus alkaline xylanase by introducing amino acid substitutions both on catalytic cleft and protein surface (2009)
  • Author(s): Hirohito Umemoto, 他
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 965-967
  • Peer Reviewed
• [Journal Article] Additional carbohydrate-binding modules enhance the insoluble substrate-hydrolytic activity of beta-1,3-glucanase from alkaliphilic Nocardiopsis sp.F96 (2009)
  • Author(s): Naoya Koizumi, 他
  • Journal Title: Biosci.Biotechnol.Biochem. 73 Pages: 1078-1082
  • Peer Reviewed
• [Presentation] CBMファミリー36キシラン結合ドメインの基質結合機構と2価金属イオンの役割 (2011)
  • Author(s): 谷澤里沙, 他
  • Organizer: 触媒学会
  • Place of Presentation: 北見工業大学(北海道)
  • Year and Date: 2011-09-20
• [Presentation] ヘミセルロースの有効利用に向けたアルカリキシラナーゼの機能向上 (2010)
  • Author(s): 中村聡
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-30
• [Presentation] Mutational analysis of a CBM family 5 chitin-binding domain of alkaline chitinase (2010)
  • Author(s): F. Uni, S. Lee, R. Yatsunami, T. Fukui and S. Nakamura
  • Organizer: TOCAT6
  • Place of Presentation: Sapporo
• [Presentation] Mutational analysis of a CBM family 5 chitin-binding domain of alkaline chitinase (2010)
  • Author(s): Fumiya Uni, 他
  • Organizer: 国際会議TOCAT6/APCAT5
  • Place of Presentation: 札幌コンベンションセンター
• [Presentation] 立体構造に基づくアルカリキシラナーゼの機能ドルイン解析と好アルカリ性向上 (2009)
  • Author(s): 中村聡, 他
  • Organizer: 第17回糖質関連酵素化学シンポジウム
  • Place of Presentation: 弘前大学
  • Year and Date: 2009-09-18
• [Book] Extremophiles Handbook (2011)
  • Author(s): S. Nakamura, K. Nakasone and T. Takashina
  • Publisher: Genetics and genomics of triangular disc-shaped halophilic archaeon Haloarcula japonica strain TR-1
• [Book] 酵素の開発と応用技術(今中忠行・監修) (2011)
  • Author(s): 中村聡(分担)
  • Total Pages: 309
  • Publisher: シーエムシー出版
• [Book] Extremophiles Handbook (Koki Horikoshi・編集) (2011)
  • Author(s): Satoshi Nakamura, 他(分担執筆)
  • Total Pages: 1247
  • Publisher: Springer
• [Book] ソフトマテリアルの応用展開(西敏夫・編),シーエムシー,大阪 (2010)
  • Author(s): 三原久和,中村聡,小畠英理
  • Publisher: ソフトマテリアルとしてのタンパク質・ペプチド
• [Book] 決定版 感動する化学 未来をひらく化学の世界 (2010)
  • Author(s): 井上晴夫, 他
  • Total Pages: 217
  • Publisher: 東京書籍
• [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization (2009)
  • Author(s): Y. Zhang, R. Yatsunami, Y. Hatori, M. Sato, K. Orishimo, T. Fukui and S. Nakamura
  • Publisher: Halotolerancy and organic-solvent tolerancy of haloarchaeal chitinase
• [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization (2009)
  • Author(s): W. Tsukimura, K. Watanabe, C. Morokuma, Ihsanawati, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka and S. Nakamura
  • Publisher: Improvement of alkaliphily of family 10 xylanase from hyperthermophile Thermotoga maritima
• [Book] Biotechnology of Lignocellulose Degradation and Biomass Utilization (2009)
  • Author(s): R. Yazawa, J. Takakura, T. Sakata, H. Miyakubo, Ihsanawati, H. Umemoto, R. Yatsunami, T. Fukui, T. Kumasaka, N. Tanaka, and S. Nakamura
  • Publisher: Mutational analysis of CBM family 36 xylan-binding domain of alkaline xylanase
• [Remarks]
  • URL: http://www.nakamura.bio.titech.ac.jp/
• [Remarks]
  • URL: http://www.nakamura.bio.titech.ac.jp/
• [Remarks]
  • URL: http://www.nakamura.bio.titech.ac.jp/
• [Remarks]
  • URL: http://www.nakamura.bio.titech.ac.jp/