Tissue formation of Wolffian duct cells, and generation of ureteric buds competent for nephron induction

• Project/Area Number: 21500424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Shinshu University
• Principal Investigator: JOHKURA Kohei 信州大学, 医学部, 准教授 (30303473)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 中腎管 / FGF / レセプター / 成長因子 / 腎臓 / 再生 / 組織工学 / 相互作用 / FGFレセプター / 尿管芽 / FGFシグナル / GDNFレセプター

Research Abstract

Wolffian duct is an essential tissue for kidney development, and ductal tissue formation of cultured Wolffian duct cells will be a key for kidney regeneration. In the present study, we examined gene expression of various grow factors in mesonephric tissues and their effects on Wolffian duct culture to assess their application for tissue engineering of the kidney. As a result, the potential of FGF was elucidated. FGF9 was shown to be expressed in mesonephric tissues and support the survival, proliferation and competence of Wolffian duct cells. A positive effect of FGF9 on primary culture of Wolffian duct cells was also verified.

Research Products

• [Journal Article] A key inactivation factor of HeLa cell viability by a plasma flow (2011)
  • Author(s): Takehiko Sato, Mayo Yokoyama, Kohei Iohkura
  • Journal Title: Journal of Physics D : Applied Physics Volume: 44(37) Issue: 37 Pages: 372001-372001
  • DOI: 10.1088/0022-3727/44/37/372001
  • Peer Reviewed
• [Journal Article] Protein kinase A regulates GDNF/Ret-dependent but not GDNF/Ret-independent ureteric bud outgrowth from the Wolffian duct (2010)
  • Author(s): Tee JB, Choi Y, Shah MM, Dnyanmote A, Sweeney DE, Gallegos TF, Johkura K, Ito C, Bush KT, Nigam SK
  • Journal Title: Dev Biol Volume: 347 Issue: 2 Pages: 337-347
  • DOI: 10.1016/j.ydbio.2010.08.029
  • Peer Reviewed
• [Journal Article] Constructing kidney-like tissues from cells based on programs for organ development : towards a method of in vitro engineering of the kidney (2010)
  • Author(s): Rosines E, Johkura K, Zhang X, Schmidt HJ, Decambre M, Bush KT, Nigam SK
  • Journal Title: Tissue Eng Part A Volume: 16 Issue: 8 Pages: 2441-2455
  • DOI: 10.1089/ten.tea.2009.0548
  • Peer Reviewed
• [Journal Article] Protein kinase A regulates GDNF/Ret-dependent but not GDNF/Ret-independent ureteric bud outgrowth from the Wolffan duct. (2010)
  • Author(s): Tee JB, 他9名、7番目
  • Journal Title: Developmental Biology Volume: 347 Pages: 337-347
  • Peer Reviewed
• [Journal Article] Constructing kidney-like tissues from cells based on programs for organ development: towards a method of in vitro engineering of the kidney. (2010)
  • Author(s): Rosines E, Johkura K(共同筆頭著者), 他5名
  • Journal Title: Tissue Engineering PartA Volume: 16 Pages: 2441-2455
  • Peer Reviewed
• [Journal Article] The instructive role of metanephric mesenchyme in ureteric bud patterning, sculpting, and maturation and its potential ability to buffer ureteric bud branching defects (2009)
  • Author(s): Shah MM, Tee JB, Meyer T, Meyer-Schwesinger C, Choi Y, Sweeney DE, Gallegos TF, Johkura K, Rosines E, Kouznetsova V, Rose DW, Bush KT, Sakurai H, Nigam SK
  • Journal Title: Am J Physiol Renal Physiol Volume: 297 Issue: 5 Pages: 1330-1341
  • DOI: 10.1152/ajprenal.00125.2009
  • Peer Reviewed
• [Journal Article] The instructive role of metanephric mesenchyme in ureteric bud patterning, sculpting, and maturation and its potential ability to buffer ureteric bud branching defects. (2009)
  • Author(s): Shah MM, (他13名、8番目)
  • Journal Title: American Journal of Physiology Renal Physiology 297
  • Peer Reviewed
• [Journal Article] Bone morphogenetic protein-4 promotes induction of cardiomyocytes from human embryonic stem cells in serum-based embryoid body development. (2009)
  • Author(s): Takei S, (他7名、3番目、責任執筆者)
  • Journal Title: American Journal of Physiology Heart Circulatory Physiology 296
• [Presentation] 腎臓原基上皮系のコンピテンス維持におけるFGFシグナル (2012)
  • Author(s): 城倉浩平、櫻井裕之
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 甲府
  • Year and Date: 2012-03-27
• [Presentation] 腎臓原基上皮系のコンピテンス維持におけるFGFシグナル (2012)
  • Author(s): 城倉浩平, 櫻井裕之
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学(甲府市)
  • Year and Date: 2012-03-27
• [Presentation] 中腎管のGDNF受容体発現と細胞増殖におけるFGFシグナルの役割 (2011)
  • Author(s): 城倉浩平、櫻井裕之、Kevin Bush、Sanjay Nigam
  • Organizer: 第88回日本生理学会大会,第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: J Physiol Sci誌上誌上開催(横浜での集会は震災ため中止)
• [Presentation] Role of FGF signal in GDNF receptor expression and cell proliferation of Wolffian duct. (2011)
  • Author(s): 城倉浩平、櫻井裕之、Kevin Bush, Sanjay Nigam
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: 横浜市での集会は震災のため中止。誌上発表J Physiol Sci 61(Supp1.1.), S272, 2011
• [Presentation] 中腎管の細胞増殖・GDNF応答性の維持におけるFGFの関与 (2010)
  • Author(s): 城倉浩平、櫻井裕之、白澤佐季子、横山忠幸、Kevin Bush、Sanjay Nigam
  • Organizer: 日本解剖学会第70回中部支部学術集会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-10-17
• [Presentation] FGFシグナルは単離中腎管の増殖とGDNF応答性を維持する (2010)
  • Author(s): 城倉浩平、櫻井裕之、白澤佐季子、横山忠幸、Kevin Bush、Sanjay Nigam
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-18
• [Presentation] FGFシグナルは単離中腎管の増殖とGDNF応答性を維持する (2010)
  • Author(s): 城倉浩平、櫻井裕之、白澤佐季子、横山忠幸、Kevin Bush、Sanjay Nigam
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2010-03-18
• [Presentation] 中腎管の細胞増殖・GDNF応答性の維持におけるFGFの関与 (2010)
  • Author(s): 城倉浩平、櫻井裕之、白澤佐季子、横山忠幸、Kevin Bush、Sanjay Nigam
  • Organizer: 日本解剖学会第70回中部支部学術集会
  • Place of Presentation: 岐阜市