| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Using a cell-sheet staking 3-D co-culture system, the mechanisms of prevascular network formation within layered cell-sheet and neovasvularization by engrafted prevascular cell-sheets were investigated. Two human genes encoding secreted glycoprotein and intracellular protein, expressed in endothelial cells (Ecs), were newly identified. Especially, EC network formation on Matrigel was inhibited by treating the cells with siRNA for the latter gene, indicating that this gene might be one of the regulators for prevascular network formation. On the other hand, the productions of both placenta growth factor (PIGF) and matrix metalloproteinase-9 (MMP-9) significantly increased in the prevascular cell-sheets. Therefore, these soluble proteins might play an important role in the functional anastomosis after subcutaneous transplantation.
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