Molecular mechanisms of glucose metabolism induced by stretch in skeletal muscles.

• Project/Area Number: 21500687
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied health science
• Research Institution: University of Shizuoka
• Principal Investigator: OBARA Kazuo 静岡県立大学, 薬学部, 講師 (60117611)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 骨格筋 / 糖代謝 / ストレッチ / インテグリン / カルシウム / GLUT4 / CaMKII / FAK / L6細胞 / 三次元培養 / NOS / シグナル伝達 / マウスヒラメ筋 / Akt / PI3K / カベオリン3

Research Abstract

Mechanism of glucose uptake induced by passive stretch stimulation(stretch) in skeletal muscle was investigated. Our results suggest that stretch is accepted by integrin/FAK and causes Ca^<2+> release from sarcoplasmic reticulum, resulting increase in glucose uptake through CaMKII activation. Our results also suggest that stretch-induced glucose uptake involves insulin-and contraction-independent pathways.

Research Products

• [Journal Article] Mechanism of potentiation by tea epigallocatechin of contraction in porcine coronary artery : The role of protein kinase Cδ-mediated CPI-17 phosphorylation (2011)
  • Author(s): Kazuo Obara, Kiyotaka Ukai, Tomohisa Ishikawa
  • Journal Title: European Journal of Pharmacology Volume: 668 Pages: 414-418
  • Peer Reviewed
• [Journal Article] Interactive role of protein phosphatase 2A and protein kinase Cα in the stretch-induced triphosphorylation of myosin light chain in canine cerebral artery (2010)
  • Author(s): Kazuo Obara, Ayako Mitate, Kayo Nozawa, Makiko Watanabe, Yoshihiko Ito, Koichi Nakayama
  • Journal Title: Journal of Vascular Research Volume: 47 Pages: 115-127
  • Peer Reviewed
• [Journal Article] Interactive role of protein phosphatase 2A and protein kinase Ca in the stretch-induced triphosphorylation of myosin light chain in canine cerebral artery. (2010)
  • Author(s): Kazuo Obara
  • Journal Title: Journal of Vascular Research Volume: 47 Pages: 115-127
  • Peer Reviewed
• [Journal Article] Interactive role of protein phosphatase 2A and protein kinase Cα in the stretch-induced triphosphorylation of myosin light chain in canine cerebral artery (2010)
  • Author(s): Kazuo Obara
  • Journal Title: Journal of Vascular Research 47 Pages: 115-127
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Koichi Nakayama
  • Journal Title: Mechanosensitivity in Cells and Tissues 3. Mechanosensitivity of the Heart(Springer) Pages: 453-481
• [Presentation] マウスヒラメ筋におけるエピガロカテキンガレートによる糖取り込みへの過酸化水素の関与について (2011)
  • Author(s): 小原一男
  • Organizer: 静岡県立大学USフォーラム
  • Place of Presentation: 静岡
  • Year and Date: 2011-09-27
• [Presentation] イヌ脳底動脈平滑筋における緑茶カテキンの収縮増強作用 (2011)
  • Author(s): 小原一男
  • Organizer: 第53回日本平滑筋学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-08-03
• [Presentation] マウスヒラメ筋における伸展刺激誘発性糖取り込みへのインテグリン/FAK経路の関与 (2011)
  • Author(s): 滝口茂幸
  • Organizer: 第124回日本薬理学会関東部会
  • Place of Presentation: 東京
  • Year and Date: 2011-06-04
• [Presentation] マウスヒラメ筋における伸展刺激誘発性糖取り込み機構 (2011)
  • Author(s): 宇田宗晃
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 茶カテキンの血管平滑筋収縮増強作用における過酸化水素の役割 (2010)
  • Author(s): 小原一男
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京
  • Year and Date: 2010-12-04
• [Presentation] マウスヒラメ筋における伸展誘発性グルコース取り込み機構の解明 (2010)
  • Author(s): 宇田宗晃
  • Organizer: 平成22年度日本薬学会東海支部例会
  • Place of Presentation: 静岡
  • Year and Date: 2010-11-28
• [Presentation] 人工骨格線維におけるエピガロカテキンがレートの糖取り込み作用について (2010)
  • Author(s): 小原一男
  • Organizer: 静岡県立大学USフォーラム
  • Place of Presentation: 静岡
  • Year and Date: 2010-08-03
• [Presentation] 人工筋線維におけるエピガロカテキンガレートの糖取り込み作用 (2010)
  • Author(s): 小原一男
  • Organizer: 静岡県立大学USフォーラム2010
  • Place of Presentation: 静岡
  • Year and Date: 2010-08-03
• [Presentation] Differential role of stretch-activated molecules involved in myogenic response and cerebral vasospasm (2010)
  • Author(s): Koichi Nakayama
  • Organizer: 12th Symposium on vascular Neuroeffector mechanism
  • Place of Presentation: Odense, Denmark
  • Year and Date: 2010-07-25
• [Presentation] Differential role of stretch-activated molecules involved in myogenic response and cerebral vasospasm. (2010)
  • Author(s): Nakayama Koichi
  • Organizer: 12th Symposium on Vascular Neuroeffector Mechanism
  • Place of Presentation: Odense, Denmark
  • Year and Date: 2010-07-25
• [Presentation] Inhibitory role of phosphorylation of myosin light chain at threonine-9 in the mechanical activity of canine basilar artery. (2010)
  • Author(s): Obara Kazuo
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2010-07-22
• [Presentation] Inhibitory role of phosphorylation of myosin light chain at threonine-9 in the mechanical activity of canine basilar artery (2010)
  • Author(s): Kazuo Obara
  • Organizer: 16th World Congress on Basic and Clinical Pharmacology
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2010-07-20
• [Presentation] ブタ冠動脈平滑筋における緑茶カテキンの収縮増強機構について (2010)
  • Author(s): 鵜飼聖隆
  • Organizer: 第52回日本平滑筋学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2010-07-02
• [Presentation] 犬脳底動脈の伸展誘発性収縮におけるミオシン輕鎖3リン酸化の役割 (2010)
  • Author(s): 小原一男
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-19
• [Presentation] イヌ脳底動脈の伸展誘発性収縮におけるミオシン輕鎖3リン酸化の役割 (2010)
  • Author(s): 小原一男
  • Organizer: 第87回に本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-19
• [Presentation] 血管平滑筋に対する緑茶カテキンの作用 (2010)
  • Author(s): 小原一男
  • Organizer: 第39回日本心脈管作動物質学会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-02-05
• [Presentation] 血管平滑筋に対する茶カテキンの作用について (2009)
  • Author(s): 小原一男
  • Organizer: 筋生理の集い
  • Place of Presentation: 東京
  • Year and Date: 2009-12-19
• [Presentation] 人工筋線維における糖取り込みに対するストレッチ刺激の影響について (2009)
  • Author(s): 小原一男
  • Organizer: 静岡県立大学2007USフォーラム
  • Place of Presentation: 静岡
  • Year and Date: 2009-08-04
• [Presentation] Involvement of protein kinase Cα in the stretch-induced triphosphorylation of myosin light chain in canine cerebral artery (2009)
  • Author(s): Kazuo Obara
  • Organizer: XXXVI International Congress of Physiological Sciences
  • Place of Presentation: 京都
  • Year and Date: 2009-07-31
• [Presentation] Role of triphosphorylation of myosin light chain in the contraction of the canine basilar artery (2009)
  • Author(s): Kazuo Obara
  • Organizer: Satellite Symposium of the IUPS2009
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-23
• [Presentation] ブタ冠動脈平滑筋収縮に対するカテキン類の効果 (2009)
  • Author(s): 鵜飼聖隆
  • Organizer: 第51回日本平滑筋学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-22
• [Book] Specific Mechano-transduction signaling involved in myogenic response of the cerebral arteries. Mechanosensitivity in Cells and Tissues (2009)
  • Author(s): Koichi Nakayama, Kazuo Obara, Tomohisa Ishikawa, Shigeru Nishizawa
  • Publisher: Springer