Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades

Research Project

Project/Area Number	21500690
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied health science
Research Institution	Health Sciences University of Hokkaido
Principal Investigator	IIZUKA Kenji 北海道医療大学, 薬学部, 准教授 (10344467)
Co-Investigator(Kenkyū-buntansha)	HIRAFUJI Masahiko 北海道医療大学, 薬学部, 教授 (20142987) MACHIDA Takuji 北海道医療大学, 薬学部, 講師 (90433424)
Project Period (FY)	2009 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	生活習慣病 / 骨格筋細胞 / MCT4 / バイオマーカー / 抗体
Research Abstract	Two types of antibodies, anti-monocarboxylate transporter(MCT) 4 polyclonal antibody recognizes C-terminal end of MCT4 and the anti-MCT4 polyclonal antibody created by considering synthetic peptide of the intermediate part of MCT4 molecule as an antigen, were prepared. Using these antibodies, we constructed both a single and a sandwiche enzyme-linked immunosorbent assay system for quantification of MCT4 concentration in serum and/or culture medium.

Report

(4 results)