Experimental design of ELISA for quantitative MCT4 analysis and an investigation of signal transduction cascades
| Project/Area Number |
21500690
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied health science
|
|---|
| Research Institution | Health Sciences University of Hokkaido |
|---|
Principal Investigator |
IIZUKA Kenji 北海道医療大学, 薬学部, 准教授 (10344467)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
HIRAFUJI Masahiko 北海道医療大学, 薬学部, 教授 (20142987)
MACHIDA Takuji 北海道医療大学, 薬学部, 講師 (90433424)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 生活習慣病 / 骨格筋細胞 / MCT4 / バイオマーカー / 抗体 |
|---|
| Research Abstract |
Two types of antibodies, anti-monocarboxylate transporter(MCT) 4 polyclonal antibody recognizes C-terminal end of MCT4 and the anti-MCT4 polyclonal antibody created by considering synthetic peptide of the intermediate part of MCT4 molecule as an antigen, were prepared. Using these antibodies, we constructed both a single and a sandwiche enzyme-linked immunosorbent assay system for quantification of MCT4 concentration in serum and/or culture medium.
|
Report
(4 results)
Research Products
(1 results)
