Role of Saposin B in the intestinal absorption of coenzyme Q10.
Project/Area Number |
21500698
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied health science
|
Research Institution | Tokyo University of Technology |
Principal Investigator |
KASHIBA Misato 東京工科大学, 医療保健学部, 講師 (80338186)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Yorihiro 東京工科大学, 応用生物学部, 教授 (60134475)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 老化 / 脂質 / 食品 / タンパク質 / 抗酸化物質 / 加齢 / 蛋白質 |
Research Abstract |
We previously reported that saposin B binds coenzyme Q10 and makes this lipid soluble in water. In order to clarify the role of saposin B in intestine, we used Caco-2 cell line. Saposin B is produced from its precursor protein, prosaposin. We produced prosaposin knockdown caco-2 cell line. Coenzyme Q10 contents in prosaposin knockdown caco-2 cell line was lower than that in control cell line. Furthermore, prosaposin knockdown resulted in the loss of transepithelial resistance and disruption of tight junctions.
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Report
(4 results)
Research Products
(15 results)