| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
We previously reported that saposin B binds coenzyme Q10 and makes this lipid soluble in water. In order to clarify the role of saposin B in intestine, we used Caco-2 cell line. Saposin B is produced from its precursor protein, prosaposin. We produced prosaposin knockdown caco-2 cell line. Coenzyme Q10 contents in prosaposin knockdown caco-2 cell line was lower than that in control cell line. Furthermore, prosaposin knockdown resulted in the loss of transepithelial resistance and disruption of tight junctions.
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