| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Hypertriglyceridemia is one of the risk factors for coronary heart disease, and it is caused by an abnormality of lipoprotein lipase(LPL) that is mainly associated with catabolism of triglyceride in the circulation. In fact, we found a compound heterozygous LPL deficiency of S193R/I194T as a cause of hypertriglyceridemia in a subject with the low level of LPL activity and LPL protein. However, there was a case whose LPL gene had no abnormality in spite of the low level of LPL both activity and protein. In this case, we found an inhibitor of LPL enzyme in the patient's plasma. Detection of the inhibitor was done by a combination of the method of measuring inhibitory effect of the plasma on LPL activity and determining a typeof the immunoglobulin binding to LPL protein by Western blot method. Measurement of the inhibition of the functional LPL activity by plasma was essential for the detection of inhibitors of LPL enzyme.
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