| Project/Area Number |
21500797
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Tokyo Kasei Gakuin University (2011)
Nigata University of Phermacy and Applied Life Sciences (2009-2010) |
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Principal Investigator |
MIYAKE Noriko 東京家政学院大学, 現代生活学部, 准教授 (70314573)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Emiko お茶の水女子大学, 大学院・人間文化創生科学研究科, 教授 (80154524)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ビタミンC / 酸化 / メイラード反応 / 加熱調理 / AGE受容体 / 安全性 / 培養細胞 / 細胞増殖 / α-カルボニル化合物 / 食品 |
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| Research Abstract |
The health effects of food-derived glycated casein by ascorbic acid(AsA) oxidation products were in vitro investigated. AsA, 2, 3-diketogulonic acid(DKG), glucose, glyoxal(GO) and methylglyoxal(MGO) were heated with casein, and their yields of glycated products were determined. Formation of DKG-casein was at similar level to GO-and MGO-casein. The cytotoxicity of DKG-casein was examined using intestinal cell line Caco-2.The larger inhibitory effect in Caco-2 cellular proliferation by DKG-casein was observed than GO-and MGO-casein. The expression of the receptor for advanced glycation endproducts(RAGE) mRNA in Caco-2 was studied. Stimulation of Caco-2 with DKG-casein resulted in a significant increase in RAGE mRNA expression. Therefore, dietary ascorbic acid-derived glycation products might have a risk for human health and the effect was suggested to be mediated by RAGE.
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