Screening and Identification of anti-mycobacterial compounds from polyphenols.

• Project/Area Number: 21500806
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Sonoda Women's University
• Principal Investigator: OZEKI Yuriko 園田学園女子大学, 健康科学部, 教授 (00169301)
• Co-Investigator(Kenkyū-buntansha): NIKI Mamiko (YOSIMURA Mamiko) 大阪市立大学, 大学院・医学研究科, 助教 (20438229) ; TAMARU Aki 大阪府立公衆衛生研究所, 感染症部, 主任研究員 (70270767)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 結核 / ポリフェノール / 免疫 / 細菌(抗酸菌) / 食品 / 微生物 / 抗生物質 / 生理活性 / 細菌

Research Abstract

We carried out screening of anti-mycobacterial compounds from polyphenols. Some polyphenols suppressed proliferation of Mycobacterium tuberculosis growing in macrophages at lower concentrations than those suppressed in 7H9 culture medium. These facts indicate that these polyphenols possess bacteriostatic effects against M. tuberculosis via activation of host immunity. These data suggest use of the polyphenols for prevention and treatment of tuberculosis.

Research Products

• [Journal Article] A novel mechanism of growth phase-dependent tolerance to isoniazid in mycobacteria (2012)
  • Author(s): Niki, M., Niki, M., Tateishi Y., Ozeki Y., Kirikae T, Lewin A., Inoue Y, Matsumoto M., Dahl JL., Ogura H., Kobayashi K., and Matsumoto S.
  • Journal Title: J Biol Chem in press
  • Peer Reviewed
• [Journal Article] 新しい結核ワクチ開発の展望 (2012)
  • Author(s): 松本壮吉、尾関百合子、小林和夫
  • Journal Title: 臨床と微生物 Volume: Vol39、2号 Pages: 131-136
• [Journal Article] 結核菌感染を診断するバイオマーカー (2012)
  • Author(s): 岡真優子、尾関百合子、松本壮吉、岩尾洋
  • Journal Title: 大阪市医学会雑誌 Volume: 61(印刷中)
  • NAID: 40019589154
  • Peer Reviewed
• [Journal Article] 新しい結核ワクチン開発の展望 (2012)
  • Author(s): 松本壮吉、尾関百合子、小林和夫
  • Journal Title: 臨床と微生物 Volume: 39 Pages: 43-48
• [Journal Article] Sensitivities of cipofloxacin-resistant Mycobacterium tuberculosis clinical isolates to fluoroquinolones : role of mutant DNA gyrase subunits in drug resistance (2012)
  • Author(s): Y.Suzuki, C.Nakajima, A Tamaru, H.Kim, et al
  • Journal Title: International Journal of Antimicrobial Agents Volume: 39 Issue: 5 Pages: 435-439
  • DOI: 10.1016/j.ijantimicag.2012.01.007
  • Peer Reviewed
• [Journal Article] Genetic diversity of Mycobacterium avium subsp. hominissuis strains isolated from humans, pigs, and human living environment (2012)
  • Author(s): D Iwamoto T, Nakajima C, Nishiuchi Y, Kato T, Yoshida S, Nakanishi N, Tamaru A, Tamura Y, Suzuki Y, Nasu M
  • Journal Title: Infect. Genet. Evolu Volume: Vol.12 Issue: 4 Pages: 846-52
  • DOI: 10.1016/j.meegid.2011.06.018
  • Peer Reviewed
• [Journal Article] Changes in salivary antimicrobial peptides, immunoglobulin A and cortisol after prolonged strenuous exercise (2012)
  • Author(s): Usui T, Yoshikawa T, Orita K, Ueda SY, Katsura Y, Fujimoto S, Yoshimura M
  • Journal Title: Eur J Appl Physiol Volume: 111 Pages: 2005-2014
  • Peer Reviewed
• [Journal Article] Loss of anti-mycobacterial efficacy in mice over time following vaccination with Mycobacterium bovis bacillus Calmette-Guerin (2011)
  • Author(s): Ozeki, Y., Y. Hirayama, T. Takii, S. Yamamoto, K. Kobayashi, and S. Matsumoto
  • Journal Title: Vaccine Volume: 29 Pages: 6881-6887
  • Peer Reviewed
• [Journal Article] A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reaction (2011)
  • Author(s): Takatsuka M, Osada-Oka M, Satoh EF, Kitadokoro K, Nishiuchi Y, Niki M, et al.
  • Journal Title: PLoS One Volume: 6 Pages: 20985-20985
  • Peer Reviewed
• [Journal Article] Structure and Hos t Recogn i t i o n o f S e r o t ype 1 3 Glycopeptidolipid from Mycobacterium intracellulare (2011)
  • Author(s): Naka T, Nakata N, Maeda S, Yamamoto R, Doe M, Mizuno S, Niki M, et al.
  • Journal Title: J Bacteriol Volume: 93 Pages: 5766-5774
  • Peer Reviewed
• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis (2011)
  • Author(s): Yukitake H., M. Naito, K. Sato, M. Shoji, N. Ohara, M. Yoshimura, et al.
  • Journal Title: Microb Immun Volume: 55 Pages: 141-153
  • NAID: 10031121453
  • Peer Reviewed
• [Journal Article] The Lipid Phenotype of two Distinct Subpopulations of Mycobacterium bovis Bacillus Calmette-Guerin Tokyo 172 Substrain (2011)
  • Author(s): Naka T, Maeda S, Niki M, Ohara N, Yamamoto S, Yano I, Maeyama J, et al.
  • Journal Title: J Biol Chem Volume: 286 Pages: 44153-44161
  • Peer Reviewed
• [Journal Article] Changes in salivary antimicrobial peptide, immunoglobulin A and cortisol after prolonged strenuous exercise (2011)
  • Author(s): Usui T., T. Yoshikawa, K. Orita, SY. Ueda, Y. Katsura, S. Fijimoto, and M. Yoshimura
  • Journal Title: Eur J Appl Physiol Volume: 111 Pages: 2005-2014
  • Peer Reviewed
• [Journal Article] 結核研究の新たな展開-潜在性結核と結核菌:休眠現象の分子メカニズム (2011)
  • Author(s): 仁木満美子、仁木誠、尾関百合子、岡真優子、松本壮吉
  • Journal Title: 最新医学 Volume: Vol66、3号 Pages: 149-155
  • NAID: 40018747670
• [Journal Article] Loss of anti-mycrobial efficacy in mice over time following vaccination with Mycobacterium bovis bacillus Calmette-Guerin (2011)
  • Author(s): Y.Ozeki, Y.Hirayama, T.Takii, S.Yamamoto, et al
  • Journal Title: Vaccine Volume: 29 Issue: 40 Pages: 6881-6887
  • DOI: 10.1016/j.vaccine.2011.07.051
  • Peer Reviewed
• [Journal Article] Structure and Host Recognition of Serotype 13 Glycopeptidolipid from Mycobacterium intracellulare (2011)
  • Author(s): Naka T, Nakata N, Maeda S, Yamamoto R, Doe M, Mizuno S, Niki M, et al
  • Journal Title: J Bacteriol Volume: 193 Issue: 20 Pages: 5766-5774
  • DOI: 10.1128/jb.05412-11
  • Peer Reviewed
• [Journal Article] A histone-like protein of mycobacteria possesses ferritin superfamily protein-like activity and protects against DNA damage by Fenton reaction (2011)
  • Author(s): Takatsuka M, Osada-Oka M, Satoh EF, Kitadokoro K, Nishiuchi Y, Niki M, et al
  • Journal Title: PLoS One Volume: 6 Issue: 6 Pages: e20985-e20985
  • DOI: 10.1371/journal.pone.0020985
  • Peer Reviewed
• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis (2011)
  • Author(s): Yukitake H, Naito M, Sato K, Shoji M, Ohara N, Yoshimura M, et al
  • Journal Title: Microbiol Immunol Volume: 55 Issue: 3 Pages: 141-153
  • DOI: 10.1111/j.1348-0421.2010.00299.x
  • NAID: 10031121453
  • Peer Reviewed
• [Journal Article] Effects of non-iron metalloporphyrins on growth and gene expression of Porphyromonas gingivalis. (2011)
  • Author(s): Yukitake H,. M.Naito, K.Sato, M.Shoji, N.Ohara, M.Yoshimura, et. al.
  • Journal Title: Microb Immun Volume: 55 Pages: 141-153
  • NAID: 10031121453
  • Peer Reviewed
• [Journal Article] Changes in salivary antimicrobial peptides, immunoglobulin A and cortisol after prolonged strenuous exercise. (2011)
  • Author(s): Usui T., T.Yoshikawa, K.Orita, SY.Ueda, Y.Katsura, S.Fijimoto, M.Yoshimura
  • Journal Title: Eur J Appl Physiol Volume: (in print)
  • Peer Reviewed
• [Journal Article] 結核研究の新たな展開:潜在性結核と結核菌-休眠現象の分子メカニズム (2011)
  • Author(s): 仁木満美子、仁木誠、尾関百合子、岡真優子、松本壮吉
  • Journal Title: 最新医学 Volume: 66 Pages: 511-517
  • NAID: 40018747670
• [Journal Article] Transient role of CD4+ CD25+ regulatory T cells in mycobacterial infection in mice (2010)
  • Author(s): Ozeki, Y., I. Sugawara, T. Udagawa, T. Aoki, M. Osada-Oka, Y. Tateishi, H. Hisaeda, Y. Nishiuchi, N. Harada, K. Kobayashi, and S. Matsumoto
  • Journal Title: Int Immunol Volume: 22 Pages: 179-189
  • NAID: 10027391706
  • Peer Reviewed
• [Journal Article] Tetratricopeptide repeat proteinassociatedproteins contribute to the virulence of Porphyromonas gingivalis (2010)
  • Author(s): Kondo Y., N. Ohara, K. Sato, M. Yoshimura, et al.
  • Journal Title: Infect Immun Volume: 78 Pages: 2846-2856
  • Peer Reviewed
• [Journal Article] Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR (2010)
  • Author(s): Nakajima C, Rahim Z, Fukushima Y, SugawaraI, Zanden A G, Tamaru A, Suzuki Y.
  • Journal Title: BMC Infect Dis Volume: 10 Pages: 118-124
  • NAID: 120002232150
  • Peer Reviewed
• [Journal Article] A novel method for the purification of DNA by capturing nucleic acid and magnesium complexes on non-woven fabric filters under alkaline conditions for the gene diagnos is of tuberculosis by loop-mediated Isothermal amplification(LAMP) (2010)
  • Author(s): Fukasawa T, Oda N, Wada Y, Tamaru A, Fukushima Y, Nakajima C, Suzuki Y.
  • Journal Title: Jpn J Infect Dis Volume: 63 Pages: 246-25
  • Peer Reviewed
• [Journal Article] Tetratricopeptide repeat protein-associated proteins contribute to the virulence of Porphyromonas gingivalis. (2010)
  • Author(s): Kondo Y., N.Ohara, K.Sato, M.Yoshimura, et. al.
  • Journal Title: Infect Immun Volume: 78 Pages: 2846-2856
  • NAID: 120006984317
  • Peer Reviewed
• [Journal Article] Identification of Mycobacterium tuberculosis clinical isolates in Bangladesh by a species distinguishable multiplex PCR. (2010)
  • Author(s): Nakajima C, Rahim Z, Fukushima Y, Sugawara I, Zanden A G, Tamaru A, Suzuki Y.
  • Journal Title: BMC Infectious Diseases Volume: 10 Pages: 118-124
  • NAID: 120002232150
  • Peer Reviewed
• [Journal Article] A novel method for the purification of DNA by capturing nucleic acid and magnesium complexes on non-woven fabric filters under alkaline conditions for the gene diagnosis of tuberculosis by loop-mediated isothermal amplification(LAMP). (2010)
  • Author(s): Fukasawa T, Oda N, Wada Y, Tamaru A, Fukushima Y, Nakajima C, Suzuki Y.
  • Journal Title: Jpn J Infect Dis Volume: 63 Pages: 246-250
  • Peer Reviewed
• [Journal Article] Transient role of CD4+CD25+ regulatory T cells in mycobacterial infection in mice. (2010)
  • Author(s): Ozeki, Y., S.Matsumoto, et al.
  • Journal Title: Int Immunol 22 Pages: 178-189
  • NAID: 10027391706
  • Peer Reviewed
• [Journal Article] Mycobacteria Exploit Host Hyaluronan for Efficient Extracellular Replication (2009)
  • Author(s): Hirayama, Y., M. Yoshimura, Y. Ozeki, I. Sugawara, T. Udagawa, S. Mizuno, N. Itano, K. Kimata, A. Tamaru, H. Ogura, K. Kobayashi, and S. Matsumoto
  • Journal Title: PLoS Pathog Volume: 5 Pages: 1000643-1000643
  • Peer Reviewed
• [Journal Article] Virulence of Mycobacterium avium complex strains isolated from immune-competent patients (2009)
  • Author(s): Tateishi, Y., Y. Hirayama, Y. Ozeki, Y. Nishiuchi, M. Yoshimura, J. Kang, A. Shibata, K. Hirata, K. Kitada, S. Kitada, R. Maekura, H. Ogura, K. Kobayashi, and S. Matsumoto
  • Journal Title: Microb Pathog Volume: 46 Pages: 6-12
  • Peer Reviewed
• [Journal Article] Mycobacterium avium complex organisms predominantly colonize in the bathtub inlets of patients bathrooms (2009)
  • Author(s): Nishiuchi, Y., A. Tamura, S. Kitada, T. Taguri, S. Matsumoto, Y. Tateishi, M. Yoshimura, Y. Ozeki, N. Matsumura, H. Ogura, and R. Maekura
  • Journal Title: Jpn J Infect Dis Volume: 62 Pages: 182-186
  • Peer Reviewed
• [Journal Article] Mycobacteria Exploit Host Hyaluronan for Efficient Extracellular Replication. (2009)
  • Author(s): Hirayama, Y., M.Yoshimura, Y.Ozeki, A.Tamaru, et al.
  • Journal Title: PLoS Pathog 5
  • Peer Reviewed
• [Journal Article] Virulence of Mycobacterium avium complex strains isolated from immunocompetent patients. (2009)
  • Author(s): Tateishi, Y., Y.Ozeki, et al.
  • Journal Title: Microb Pathog 46 Pages: 6-12
  • Peer Reviewed
• [Journal Article] Mycobacterium avium Complex Organisms Predominantly Colonize in the Bathtub Inlets of Patients' Bathrooms. (2009)
  • Author(s): Nishiuchi, Y., Y.Ozeki, et al.
  • Journal Title: Jpn J Infect Dis 62 Pages: 182-186
  • Peer Reviewed
• [Presentation] マウスモデルにおけるBCGワクチン効果の減衰に関する研究 (2012)
  • Author(s): 尾関百合子、平山幸雄、岡真優子、立石善隆、瀧井猛将, 他
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] 休眠時における抗酸菌の遺伝子発現解析 (2012)
  • Author(s): 仁木満美子、仁木誠、小林和夫
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] マクロファージのグルコース代謝と宿主内結核菌の増殖 (2012)
  • Author(s): 岡真優子、合田亘人、曽我朋義、尾関百合子、小林和夫、松本壮吉、岩尾洋
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] ケニアの学生における、結核菌タンパク質に対する免疫応答の分析 (2012)
  • Author(s): 井上学、岡真優子、仁木満美子、尾関百合子、一瀬休生、小林和夫、松本壮吉
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] 高病原性Mycobacterium intracellulare臨床分離株のドラフトゲノム解析 (2012)
  • Author(s): 立石義隆、小倉義俊、平山幸雄、尾関百合子、西内由紀子, ほか
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Mycobacterium DNA-binding protein 1(MDP1)に見出されたFerritin-super family蛋白質様活性 (2012)
  • Author(s): 松本壮吉、岡真優子、西内由紀子、仁木満美子、尾関百合子, 他
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎
• [Presentation] Loss of anti-mycobacterial efficacy in mice over t i m e f o l l o w i n g v a c c i n a t i o n w i t h Mycobacterium bobis bacillus Calmette-Gerin (2011)
  • Author(s): Ozeki, Y., Hirayama, Y., Osada-Oka M., Takii, T., Yamamoto S., et al.
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-19
• [Presentation] Loss of anti-mycobacterial efficacy in mice over time following vaccination with Mycobacterium bobis bacillus Calmette-Gerin (2011)
  • Author(s): Ozeki, Y., Hirayama, Y., Osada-Oka M., Takii, T., Yamamoto S., et al
  • Organizer: 第64回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-19
• [Presentation] Loss of anti-mycrobial effica cy in mice over time following vaccinati on with Mycobacterium bovis bacillus Calmette-Guerin (2011)
  • Author(s): Y. Ozeki, Y. Hirayama, T. Takii, S. Yamamoto, et al.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] 患者年齢構成の異なる地域間での結核菌地域分子疫学比較 (2011)
  • Author(s): 田丸亜貴、和田崇之、岩本朋忠、吉田志緒美、野尻孝子
  • Organizer: 第86回日本結核病学会総会
  • Place of Presentation: 東京
• [Presentation] Loss of anti-mycrobial efficacy in mice over time following vaccination with Mycobacterium bovis bacillus Calmette-Guerin (2011)
  • Author(s): Y.Ozeki, Y.Hirayama, T.Takii, S.Yamamoto, et al
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo, Japan
• [Presentation] 濃度長期保持型二酸化塩素ガス溶存液の結核菌に対する抗菌作用について (2011)
  • Author(s): 田丸亜貴、福田俊昭、森野博文、三浦孝典
  • Organizer: 第86回日本結核病学会総会
  • Place of Presentation: 東京
• [Presentation] Transient role of CD4+ CD25+ regulatory T cells in Mycobacterium tuberculosis infection in mice (2010)
  • Author(s): Y. Ozeki, I. Sugawara, T. Udagawa, H. Hisaeda, K. Kobayashi, and S. Matsumoto
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe, Japan, August
• [Presentation] Transient role of CD4+ CD25+ regulatory T cells in Mycobacterium tuberculosis infection in mice (2010)
  • Author(s): Y. Ozeki, I. Sugawara, T. Udagawa, T. Aoki, M. Osada-Oka, Y. Tateishi, et al
  • Organizer: 45th US-Japan, Cooperative Medical Science Program
  • Place of Presentation: Cambridge, USA
• [Presentation] 大阪府における多発性広域感染株について (2010)
  • Author(s): 田丸亜貴、和田崇之、長谷篤、岩本朋忠、吉田志緒美、前田伸司、松本智成
  • Organizer: 第85回日本結核病学会総会
  • Place of Presentation: 京都
• [Presentation] 結核菌感染における制御性T細胞の関与 (2010)
  • Author(s): 尾関百合子、菅原勇、岡真優子、小林和夫、松本壮吉
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Transient role of CD4^+CD25^+ regulatory T cells in Mycobacterium tuberculosis infection in mice. (2010)
  • Author(s): Y.Ozeki, I.Sugawara, T.Udagawa, T.Aoki, M.Osada-Oka, Y.Tateishi, H.Hisaeda, Y.Nishiuchi, N.Harada, K.Kobayashi, S.Matsumoto.
  • Organizer: 45th US-Japan, Cooperative Medical Science Program
  • Place of Presentation: Cambridge, USA
• [Presentation] Transient role of CD4^+CD25^+ regulatory T cells in Mycobacterium tuberculosis infection in mice. (2010)
  • Author(s): Y.Ozeki. I.Sugawara, H.Hisaeda, K.Kobayashi, S.Matsumoto.
  • Organizer: 14^<th> International Congress of Immumology
  • Place of Presentation: Kobe, Japan
• [Presentation] 結核菌感染における制御性T細胞の役割 (2009)
  • Author(s): 尾関百合子、菅原勇、小林和夫、松本壮吉
  • Organizer: 第62回日本細菌学会関西支部会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-14
• [Presentation] 抗酸菌は宿主ヒアルロン酸を利用して細胞外増殖を行う (2009)
  • Author(s): 平山幸雄、吉村満美子、尾関百合子、田丸亜貴、小倉壽、小林和夫、松本壮吉
  • Organizer: 第62回日本細菌学会関西支部総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-11-14
• [Presentation] 結核菌感染における制御性T細胞の役割 (2009)
  • Author(s): 尾関百合子
  • Organizer: 第62回日本細菌学会関西支部会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2009-11-14
• [Presentation] 大阪における多剤耐性・超多剤耐性結核の疫学とその制圧に関する研究 (2009)
  • Author(s): 田丸亜貴、尾関百合子
  • Organizer: 第42回関西抗酸菌研究会
  • Place of Presentation: 大阪
  • Year and Date: 2009-06-26
• [Presentation] A ferritin superfamily-like protein in mycobacteria (2009)
  • Author(s): Mayuko Osada-Oka, Masaki Takatsuka, F Eisuke Sato, Yuriko Ozeki, Mamiko Yoshimura, Masayasu Inoue, Kazuo Kobayashi, Sohkichi Matsumoto
  • Organizer: THE9TH AWAJI INTERNATIONAL FORUM
  • Place of Presentation: 淡路島
• [Presentation] 結核菌の増殖に対するヒアルロン酸の作用 (2009)
  • Author(s): 平山幸雄、吉村満美子、尾関百合子、菅原勇、小林和夫、松本壮吉
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 急速な臨床経過に合致した高病原性Mycobacterium avium-intracellulare complex菌株の同定 (2009)
  • Author(s): 立石善隆、平山幸雄、尾関百合子、西内由紀子、吉村満美子、小林和夫、松本壮吉
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Presentation] 抗結核菌薬スクリーニング系の確立と実践 (2009)
  • Author(s): 尾関百合子、原田誠、西内由紀子、山本三郎、小林和夫、松本壮吉
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
• [Book] BCG-Vaccine and Adjuvant (2012)
  • Author(s): Takii T, Maeyama J, Yamamoto S, Niki M, et al.
  • Total Pages: 272
  • Publisher: JATA(公益財団法人結核予防会)
• [Book] BCG-Vaccine and Adjuvant (2012)
  • Author(s): Takii T, Maeyama J, Yamamoto S, Niki M, et al
  • Total Pages: 272
  • Publisher: JATA(公益財団法人結核予防会)