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Development of methods for analyzing fungal metabolites by using a portable ion mobility spectrometer under atmospheric pressure

Research Project

Project/Area Number 21500988
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cultural property science
Research InstitutionNara Women's University

Principal Investigator

TAKEUCHI Takae  奈良女子大学, 理学部, 准教授 (80201606)

Co-Investigator(Renkei-kenkyūsha) KIUCHI Masato  独立行政法人産業技術総合研究所, ユビキタスエネルギー研究部門, 主任研究員 (50356862)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsカビ臭 / 固相マイクロ抽出 / GCMS / 文化財 / 環境保全技術 / イオンモビリティースペクトロメトリー / MVOC / 微生物由来揮発性有機化合物 / イオンモビリティー / IMS / 微生物 / 文化財保存 / モニタリング / GC/MS / SPME / 文化財保全 / 微生物由来揮発性物 / イオンモビリティースペクトル / データベース
Research Abstract

Fungal odor analysis in early growth stages is important for the conservation of cultural properties. The analytical methods for detecting fungal species and the stage of growth were developed using a distinctive fungal odor[Takeuchi et al, Surf. Interface Anal., 44(6), 694-698(2012)]. Microbial volatile organic compounds were extracted by solid space microextaction, followed by GC/MS analysis. While the fungi were growing, the amount of alcohols, ketones and aldehydes as MVOCs emitted increased. The detection of sesquiterpenes was useful for determining fungal species. The prototype of solid-phase microextraction inlet system available in an ion mobility spectrometer(IMS) was manufactured. Software programs were also made for identifying fungal species by IMS spectra analysis of the odor.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2012 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 5 results) Presentation (32 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Analysis of volatile metabolites emitted by soilderived fungi using head space solid-phase microextraction/gas chromatography/mass spec-trometry : I. Aspergillus fumigatus, Aspergillus nidulans, Fusarium solani and Penicillium paneum2012

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Haruna Tanaka, Sachiyo Kaneko, Shoko Ichii, Masato Kiuchi and Takahito Suzuki
    • Journal Title

      Surf. Interface Anal

      Volume: 44(6) Issue: 6 Pages: 694-698

    • DOI

      10.1002/sia.4870

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fungal Odor Analysis for Conservation of Cultural Properties2012

    • Author(s)
      T. Takeuchi, M. Kiuchi and T. Suzuki
    • Journal Title

      J. Japan Associa-tion on Odor Environment

      Volume: 43(3)

    • NAID

      130005142314

    • Related Report
      2011 Final Research Report
  • [Journal Article] Analysis of Volatile Metabolites Emitted by Soil Fungi Using Head Space Solid-Phase Microextraction GC/MS and Ion Mobility Spectrometry2011

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Haruna Tanaka, Sachiyo Kaneko, Shoko Ichii, Masato Kiuchi, Takahito Suzuki
    • Journal Title

      Proceedings of the 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, Colorado, 5-9 June, 2011

      Volume: 59

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Effects of the silicon core structures on the hole mobility of star-shaped oligothiophenes2010

    • Author(s)
      J. Ohshita, Y. Hatanaka, S. Matsui, T. Mizumo, Y. Kunugi, Y. Honsho, A. i Saeki, S. Seki, J. Tibbelin, H. Ottosson and T. Takeuchi
    • Journal Title

      Dalton Transactions.

      Volume: 39 Issue: 39 Pages: 9314-9320

    • DOI

      10.1039/c0dt00224k

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of the silicon core structures on the hole mobility of star-shaped oligothiophenes2010

    • Author(s)
      J.Ohshita, Y.Hatanaka, S.Matsui, T.Mizumo, Y.Kunugi, Y.Honsho, A.Saeki, S.Seki, J.Tibbelin, H.Ottosson, Takae Takeuchi
    • Journal Title

      J.Chem.Soc., Dalton Transactions

      Volume: 2010 Pages: 9314-9320

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Fungi Using SPME-GC/MS of MVOCs Emitted from Aspergillus fumigatus, Aspergillus nidulans, Fusarium solani and Penicillium paneum2010

    • Author(s)
      T.Takeuchi, T.Kimura, H.Tanaka, M.Kiuchi, S.Kaneko, S.Iwaguchi, T.Suzuki
    • Journal Title

      Proceedings of the 58th ASMS Conference on Mass Spectrometry and Allied Topics

      Volume: 58

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Distinct features of matrix-assisted 6μm infrared laser desorption/ionization mass spectrometry in biomolecular snalysis2009

    • Author(s)
      M. Tajiri, T. Takeuchi and Y. Wada
    • Journal Title

      Anal. Chem.

      Volume: 81(16) Issue: 16 Pages: 6750-6755

    • DOI

      10.1021/ac900695q

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distinct Features of Matrix-Assisted 6 μm Infrared Laser Desorption/Ionization Mass Spectrometry in Biomolecular Analysis2009

    • Author(s)
      田尻道子
    • Journal Title

      Analytical Chemistry 81

      Pages: 6750-6755

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] The Effect of Incubation Temperature on Microbial Volatile Organic Compounds Emitted by Aspergillus fumigatus and Penicillium paneum2012

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Shoko Ichii, Masato Kiuchi, Sachiyo Kaneko, Takahito Suzuki
    • Organizer
      60th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Vancouver, BC, Canada
    • Year and Date
      2012-05-22
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Effect of Incubation Temperature on Microbial Volatile Organic Compounds Emitted by Aspergillus fumigatus and Penicillium paneum2012

    • Author(s)
      Takae Takeuchi, Tomoko Kimura, Shoko Ichii, Masato Kiuchi, Sachiyo Kaneko and Takahito Suzuki
    • Organizer
      60^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Vancouver(Canada)
    • Year and Date
      2012-05-21
    • Related Report
      2011 Final Research Report
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析法による土壌由来カビ代謝物の分析2012

    • Author(s)
      櫟彰子, 木村知子, 大岩さゆり, 鈴木孝仁, 木内葉子, 竹内孝江
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      横浜
    • Year and Date
      2012-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析法による土壌由来カビ代謝物の分析2012

    • Author(s)
      櫟彰子, 木村知子, 大岩さゆり, 鈴木孝仁, 木内葉子, 竹内孝江
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2012-03-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 簡易な検出器でカビ臭を解析カビ種と生育状況を推測するソフトウェアを開発-IMSによる土壌由来カビ検出データベースの構築-2012

    • Author(s)
      竹内孝江, 櫟彰子, 中村義隆, 紅朋浩, 菅井俊樹, 本城国明, 岩松雅子, 木内正人
    • Organizer
      次世代ナノテクフォーラム2012
    • Place of Presentation
      大阪
    • Year and Date
      2012-02-09
    • Related Report
      2011 Final Research Report
  • [Presentation] MSフラグメンテーションの基礎理論-準平衡理論(QET)-2011

    • Author(s)
      竹内孝江
    • Organizer
      第3回LC/MSワークショップ
    • Place of Presentation
      掛川
    • Year and Date
      2011-10-27
    • Related Report
      2011 Final Research Report
  • [Presentation] MSフラグメンテーションの基礎理論-準平衡理論(QET)-2011

    • Author(s)
      竹内孝江
    • Organizer
      第3回LC/MSワークショップ
    • Place of Presentation
      静岡県掛川市(招待講演)
    • Year and Date
      2011-10-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] ポリジメチルシロキサンを用いたカビ臭物質濃縮のための固相マイクロ抽出プローブの開発-IMSによる文化財環境モニターへの適用-2011

    • Author(s)
      竹内孝江, 本城国明, 岩松雅子, 櫟彰子
    • Organizer
      第15回ケイ素化学協会シンポジウム
    • Place of Presentation
      須磨
    • Year and Date
      2011-10-21
    • Related Report
      2011 Final Research Report
  • [Presentation] ポリジメチルシロキサンを用いたカビ臭物質濃縮のための固相マイクロ抽出プローブの開発-IMSによる文化財環境モニターへの適用-2011

    • Author(s)
      竹内孝江, 本城国明, 岩松雅子, 櫟彰子
    • Organizer
      第15回ケイ素化学協会シンポジウム
    • Place of Presentation
      兵庫県神戸市須磨区
    • Year and Date
      2011-10-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析による土壌由来カビAspergillus nidulansの揮発性代謝物の同定2011

    • Author(s)
      櫟彰子, 菅井俊樹, 木村知子, 田中春菜, 金子幸代, 木内正人, 鈴木孝仁, 竹内孝江
    • Organizer
      第59回質量分析総合討論会
    • Place of Presentation
      大阪
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] イオンモビリティースペクトロメトリーと質量分析による土壌由来カビAspergillus nidulansの揮発性代謝物の同定2011

    • Author(s)
      櫟彰子, 菅井俊樹, 木村知子, 田中春菜, 金子幸代, 木内正人, 鈴木孝仁, 竹内孝江
    • Organizer
      第59回質量分析総合討論会(2011)
    • Place of Presentation
      大阪
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] Physiological activity of microbial volatile organic compounds(MVOCs) as a growth regulator in the soil-derived fungal organisms2011

    • Author(s)
      Sachiyo Kaneko, Haruna Tanaka, Tomoko, Kimura, Takae Takeuchi, Masato Kiuchi, Shin-ichi Iwaguchi, Koji Yokoyama, Takahito Suzuki
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] 招待講演「微生物由来揮発性有機化合物の測定法2011

    • Author(s)
      竹内孝江
    • Organizer
      JAIMAコンファレンス・室内環境学会主催講演会
    • Place of Presentation
      幕張
    • Year and Date
      2011-09-08
    • Related Report
      2011 Final Research Report
  • [Presentation] 微生物由来揮発性有機化合物の測定法2011

    • Author(s)
      竹内孝江
    • Organizer
      JAIMAコンファレンス・室内環境学会主催講演会「諸環境の室内空気質とその測定法」
    • Place of Presentation
      千葉県幕張市(招待講演)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] 文化財保全環境モニター装置開発のための微生物由来揮発性代謝物MVOCの分析2011

    • Author(s)
      竹内孝江
    • Organizer
      質量分析計ユーザーズフォーラム2011
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2011-07-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of Volatile Metabolites Emitted by Soil Fungi Using Head Space Solid-Phase Microextraction GC/MS and Ion Mobility Spectrometry2011

    • Author(s)
      T. Takeuchi, T. Kimura, H. Tanaka, S. Kaneko, S. Ichii, M. Kiuchi and T. Suzuki
    • Organizer
      59^<th> ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Denver(USA)
    • Year and Date
      2011-06-06
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of Volatile Metabolites Emitted by Soil Fungi Using Head Space Solid-Phase Microextraction GC/MS and Ion Mobility Spectrometry2011

    • Author(s)
      T.Takeuchi, T.Kimura, H.Tanaka, S.Kaneko, S.Ichii, M.Kiuchi, T.Suzuki
    • Organizer
      59th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      米国デンバー市
    • Year and Date
      2011-06-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Characterization of the Soil-Derived Fungi Based on Mass Spectrometric Analysis of Their Microbial Volatile Organic Compounds2011

    • Author(s)
      T. Takeuchi, H. Tanaka, T. Kimura, S. Ichii, M. Iwamatsu, S. Kaneko, M. Kiuchi and T. Suzuki
    • Organizer
      11^<th> International Symposium on Atom Level Characterization for New Materials and Devices' 11(ALC' 11)
    • Place of Presentation
      Seoul(Korea)
    • Year and Date
      2011-05-23
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of the Soil-Derived Fungi Based on Mass Spectrometric Analysis of Their Microbial Volatile Organic Compounds2011

    • Author(s)
      Takae Takeuchi, Haruna Tanaka, Tomoko Kimura, Shouko Ichii, Masako Iwamatsu, Sachiyo Kaneko, Masato Kiuchi, Takahito Suzuki
    • Organizer
      11th International Symposium on Atomic Level Characterizations for New Materials and Devices '11 (ALC'11)
    • Place of Presentation
      大韓民国ソウル市
    • Year and Date
      2011-05-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微生物由来揮発性有機化合物の固相マイクロ抽出ガスクロマトグラフィー質量分析による真菌類のキャラクタリゼーション2011

    • Author(s)
      田中春菜, 木村知子, 金子幸代, 鈴木孝仁, 竹内孝江
    • Organizer
      日本化学会第91春季年会
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] カビの他感作用物質としての2-pentadecanone2010

    • Author(s)
      金子幸代, 田中春菜, 竹内孝江, 木内正人, 岩口伸一, 鈴木孝仁
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      愛知
    • Year and Date
      2010-09-09
    • Related Report
      2011 Final Research Report
  • [Presentation] カビの他感作用物質としての2-Pentadecanone2010

    • Author(s)
      金子幸代, 田中春菜, 竹内孝江, 木内正人, 岩口伸一, 鈴木孝仁
    • Organizer
      日本植物学会第74回大会
    • Place of Presentation
      愛知県春日井市
    • Year and Date
      2010-09-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 招待講演「マススペクトロメトリーにおける気相イオン化学と反応ダイナミックス」2010

    • Author(s)
      竹内孝江
    • Organizer
      原子衝突研究協会第35回年会
    • Place of Presentation
      奈良
    • Year and Date
      2010-08-09
    • Related Report
      2011 Final Research Report
  • [Presentation] マススペクトロメトリーにおける気相イオン化学と反応ダイナミックス2010

    • Author(s)
      竹内孝江
    • Organizer
      原子衝突研究協会第35回年会
    • Place of Presentation
      奈良(特別講演)
    • Year and Date
      2010-08-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] 中赤外レーザーを用いる脱離イオン化の特性と酸性糖質およびSニトロソ化合物への応用2010

    • Author(s)
      田尻道子, 和田芳直, 竹内孝江
    • Organizer
      第58回質量分析総合討論会(2010)
    • Place of Presentation
      つくば
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 土壌カビ由来の揮発性有機化合物の固相マイクロ抽出-ガスクロマトグラフィー質量分析によるカビの識別-Aspergillus sp.,Fusarium sp.,Penicillium sp.2010

    • Author(s)
      田中春菜, 木村知子, 長谷川美穂, 金子幸代, 鈴木孝仁, 岩口伸一, 岩松雅子, 竹内孝江
    • Organizer
      第58回質量分析総合討論会(2010)
    • Place of Presentation
      つくば
    • Year and Date
      2010-06-17
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characterization of Fungi Using SPME-GC/MS of MVOCs Emitted from Aspergillus fumigatus, Aspergillus nidulans, Fusarium solani and Penicillium paneum2010

    • Author(s)
      T.Takeuchi, T.Kimura, H.Tanaka, M.Kiuchi, S.Kaneko, S.Iwaguchi, T.Suzuki
    • Organizer
      58th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Salt Lake City, USA
    • Year and Date
      2010-05-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] 招待講演「文化財環境モニターのための小型臭気センサーの開発」2010

    • Author(s)
      竹内孝江
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 文化財環境モニターのための小型臭気センサーの開発2010

    • Author(s)
      竹内孝江
    • Organizer
      日本化学会第90春季年会
    • Place of Presentation
      近畿大学(大阪府)
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] Development of the portable mold-derived odor detecting system based on IMS technique for preventing the deterioration of the cultural propertie?s2009

    • Author(s)
      大久保衛
    • Organizer
      18th International Conference on Mass Spectrometry
    • Place of Presentation
      Bremen, Germany
    • Year and Date
      2009-08-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Microbial Volatile Organic Compounds of Aspergillus sp.2009

    • Author(s)
      竹内孝江
    • Organizer
      58th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2009-06-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aspergillus fumigatus由来の揮発性有機化合物の質量スペクトル2009

    • Author(s)
      田中春菜
    • Organizer
      第57回質量分析総合討論会
    • Place of Presentation
      大阪国際交流センター(大阪府)
    • Year and Date
      2009-05-15
    • Related Report
      2009 Annual Research Report
  • [Book] 「文化財保全技術」に関する先導的研究開発委員会活動終了報告書2010

    • Author(s)
      志水隆一
    • Total Pages
      81
    • Publisher
      独立行政法人 日本学術振興会
    • Related Report
      2010 Annual Research Report
  • [Book] Microbial volatile organic compounds(MVOCs) and their presumed physiological role for growth regulation in the soil-derived fungal organisms", Proceedings of JSPS-IIAS Joint International Symposium on Conservation Technology for Cultural Heritage2009

    • Author(s)
      S. Kaneko, H. Tanaka, T. Kimura, T. Takeuchi, M. Kiuchi, S. Iwaguchi, K. Yokoyama and T. Suzuki
    • Publisher
      International Institute for Advanced Studies, Kyoto
    • Related Report
      2011 Final Research Report
  • [Book] JSPS-IIAS Joint International Symposium on Conservation Technology for Cultural Heritage2009

    • Author(s)
      金子幸代
    • Total Pages
      86
    • Publisher
      Microbial volatile organic compounds (MVOCs) and their presumed physiological role for growth regulation in the soil-derived fungal organisms
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://koto10.nara-wu.ac.jp/Profiles/5/0000420/profile.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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