Identification and characterization of biological functions of DNA repair enzymes that recognize oxidative base damage
Project/Area Number |
21510056
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Risk sciences of radiation/Chemicals
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Research Institution | Kyoto University |
Principal Investigator |
AKIYAMA Qiu-mei (ZHANG Qiu-mei) 京都大学, 大学院・理学研究科, 准教授 (00260604)
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Co-Investigator(Kenkyū-buntansha) |
HASHIGUCHI Kazunari 京都大学, 大学院・理学研究科, 助教 (80400414)
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Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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Keywords | 放射線 / 活性酸素 / 塩基損傷 / DNA修復 / 突然変異 / 塩基除去修復 / 修復酵素 / 酸化ストレス / 酸化防御タンパク質 / 活性酵素 |
Research Abstract |
We first identified three kinds of base excision repair enzymes(Nth, APN-1 and EXO-3), pyrophosphatases for oxidized deoxyribonucleotides(NDX-1, NDX-2 and NDX-4), mismatch repair enzymes(MSH2, MSH6, MLH1 and PMS2) and OXR1 protein in the nematode C. elegans. Furthermore, the present study revealed critical roles of these proteins in prevention against oxidative stress using deficient mutant or RNAi knockdown worms. In addition, we also identified some DNA repair enzymes in the ascidian Ciona intestinalis.
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Report
(4 results)
Research Products
(136 results)
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[Journal Article] Mitochondria-Targeted Superoxide Dismutase (SOD2) Regulates Radiation Resistance and Radiation Stress Response in HeLa Cells2012
Author(s)
Hosoki, A., S. Yonekura, Q-L. Zhao, Z-L. Wei, I. Takasaki, Y. Tabuchi, L-L. Wang, S. Hasuike, T. Nomura, A. Tachibana, K. Hashiguchi, S. Yonei, T. Kondo and Q-M. Zhang-Akiyama
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Journal Title
Journal of Radiation Research
Volume: 53
Issue: 1
Pages: 58-71
DOI
NAID
ISSN
0449-3060, 1349-9157
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Peer Reviewed
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[Journal Article] Error-prone translesion DNA synthesis by Escherichia coli DNA polymerase IV(DinB) on templates containing 1,2-dihydro-2-oxoadenine.2010
Author(s)
Hori, M, Yonekura, S., Nohmi, Sugiyama, H., Yonei, S., Zhang-Akiyama, Q-M.
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Journal Title
Journal of Nucleic Acids
Volume: ID807579
Pages: 10-10
Related Report
Peer Reviewed
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[Presentation] The analysis of mismatch repair in C.elegans2011
Author(s)
Moriwaki, T., Y.Kato, S.Ishigawa, K.Hashiguchi, Q-M.Zhang-Akiyama
Organizer
5th International Symposium of the Biodiversity and Evolution Global COE Project (Kyoto University)
Place of Presentation
京都大学時計台(京都市)
Year and Date
2011-07-18
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[Presentation] Significance of mitochondrial antioxidant enzymes for protection against cellular damageby ionizing radiation2010
Author(s)
Hosoki, A., Kato, S., Yonekura, S., Nomura, T., Kondo, T., Hashiguchi, K, Zhang-Akiyama, Q-M.
Organizer
The 4th International Symposium of the Biodiversity Global COE Project(Kyoto University)
Place of Presentation
Shiran Kaikan, Kyoto University
Year and Date
2010-09-11
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[Presentation] 線虫の塩基除去修復とゲノム安定性2009
Author(s)
秋山(張)秋梅, 中村允耶, 森永浩伸, 浅井翔太, 眞田悠生, 加藤悠一, 森脇隆仁
Organizer
日本遺伝学会第81回大会
Place of Presentation
信州大学理学部(松本市)
Year and Date
2009-09-17
Related Report
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[Presentation] Repair of AP sites in C. elegans2009
Author(s)
Kato, Y., K.Hashiguchi, Q-M.Zhane-Akiyama
Organizer
The 3rd International Symposium of the Biodiversity Global COE Project
Place of Presentation
京都国際交流会館(京都市)
Year and Date
2009-07-24
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