| Project/Area Number |
21510056
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
|
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| Research Institution | Kyoto University |
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Principal Investigator |
AKIYAMA Qiu-mei (ZHANG Qiu-mei) 京都大学, 大学院・理学研究科, 准教授 (00260604)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIGUCHI Kazunari 京都大学, 大学院・理学研究科, 助教 (80400414)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 放射線 / 活性酸素 / 塩基損傷 / DNA修復 / 突然変異 / 塩基除去修復 / 修復酵素 / 酸化ストレス / 酸化防御タンパク質 / 活性酵素 |
|---|
| Research Abstract |
We first identified three kinds of base excision repair enzymes(Nth, APN-1 and EXO-3), pyrophosphatases for oxidized deoxyribonucleotides(NDX-1, NDX-2 and NDX-4), mismatch repair enzymes(MSH2, MSH6, MLH1 and PMS2) and OXR1 protein in the nematode C. elegans. Furthermore, the present study revealed critical roles of these proteins in prevention against oxidative stress using deficient mutant or RNAi knockdown worms. In addition, we also identified some DNA repair enzymes in the ascidian Ciona intestinalis.
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