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Basic study on the effect of endocrine disruptors on reptiles reproduction using a model of American alligators, Alligator Mississippiensis

Research Project

Project/Area Number 21510068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionTottori University

Principal Investigator

OHTA Yasuhiko  鳥取大学, 農学部・獣医学科, 教授 (60069078)

Co-Investigator(Kenkyū-buntansha) KATSU Yoshinao  北海道大学, 大学院・理学研究院, 准教授 (00332180)
Co-Investigator(Renkei-kenkyūsha) TAKEUCHI Takashi  鳥取大学, 農学部・獣医学科, 准教授 (10325061)
IGUCHI Taisen  大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords環境ホルモン / ハ虫類 / アメリカンアリゲーター / 性分化 / 生殖輸管 / 生殖輸管分化 / 生殖輸管系分化
Research Abstract

In American alligator showing temperature-dependent sex determination(TSD), development of reproductive organs, ontogeny of expression of estrogen and progesteronereceptor and candidates involved in the TSD were identified during the thermo-sensitiveperiod. These results may contribute to the understanding of endocrine disrupters onreptiles.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (15 results)

All 2011 2010 2009 Other

All Journal Article (8 results) (of which Peer Reviewed: 8 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] Altered gonadal expression of TGFαsuperfamily signaling factors in environmental contaminant-exposed juvenile alligators2011

    • Author(s)
      Moore, B. C., Milnes, M. R., Kohno, S., Katsu, Y., Iguchi, T., Woodruff, T. K., Guillette, L, Jr.
    • Journal Title

      J. Steroid Biochem. Mol. Biol.

      Volume: 127 Issue: 1-2 Pages: 58-63

    • DOI

      10.1016/j.jsbmb.2011.01.004

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sex steroid and thyroid hormone receptor expressions in the thyroid of the American alligator(Alligator mississippiensis) during different life stages2011

    • Author(s)
      Bermudez, D., Skotko, J. P., Ohta, Y. Boggs, S. S. P., Iguchi, T., Guillette, L. J, Jr.
    • Journal Title

      J. Morphology

      Volume: 271 Issue: 6 Pages: 698-703

    • DOI

      10.1002/jmor.10936

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecularcloning pf anti-Mullerian hormone from the American alligator, Alligator mississippiensi2011

    • Author(s)
      Urushitani, H., katsu, Y., Miyagawa, S., Kohno, S., Ohta, Y., Guillette, L. J. Jr., Iguchi, T.
    • Journal Title

      Mol. Cel. Endcrinol.

      Volume: 333 Issue: 2 Pages: 190-199

    • DOI

      10.1016/j.mce.2010.12.025

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning characterization, and chromosome mapping of reptilian estrogen receptors2010

    • Author(s)
      Katsu, Y., Matsubara, K., Kohno, S., Toriba, M., Oka, K., Guillette, L. J, Jr., Ohta, Y., Iguchi, T.
    • Journal Title

      Endocrinology

      Volume: 151 Issue: 12 Pages: 5710-5720

    • DOI

      10.1210/en.2010-0356

    • Related Report
      2011 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator2010

    • Author(s)
      Kohno, S., Katsu, Y., Urushitani, U., Ohta, Y., Iguchi, T., Guillette, L. Jr.
    • Journal Title

      Sexual Devel.

      Volume: 4 Issue: 1-2 Pages: 73-87

    • DOI

      10.1159/000260374

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential Contributions of Heat Shock Proteins to Temperature-Dependent Sex Determination in the American Alligator2010

    • Author(s)
      S.Kohno, Y.Katsu, H.Urushitani, Y.Ohta, T.Iguchi, L.J.Guillette, Jr.
    • Journal Title

      Sexual Development 4

      Pages: 73-87

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning pf anti-Mullerian hormone from the American alligator, Alligator mississippiensis

    • Author(s)
      Urushitani, H., katsu, Y., Miyagawa, S., Kohno, S., Ohta, Y., Guillette, L.J, Jr., Iguchi, T.
    • Journal Title

      Mol.Col.Endcrinol

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sex steroid and thyroid hormone receptor expressions in the thyroid of the American alligator (Alligator mississippiensis) during different life stages

    • Author(s)
      Bermudez, D., Skotko, J.P., Ohta, Y., Boggs, S.S.P., Iguchi, T., Guillette, L.J, Jr.
    • Journal Title

      J.Morphology

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] アメリカンアリゲーターのコルチコイド受容体の単離と機能解析2011

    • Author(s)
      岡香織・太田康彦・井口泰泉・勝義直
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(旭川市)
    • Year and Date
      2011-09-11
    • Related Report
      2011 Final Research Report
  • [Presentation] アメリカアリゲーターのコルチコイド受容体の単離と機能解析2011

    • Author(s)
      岡香織・太田康彦・井口泰泉・勝義直
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール(旭川市)
    • Year and Date
      2011-09-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] Which estrogen receptor(ESR1 ; ERαor EXR2 ; ERβ) is involved in sex determination and gonadal differentiation? Michigan University2011

    • Author(s)
      Kohno, S. Katus, Y. Ohta, Y. Igutchi, T., Guillette, L. J.
    • Organizer
      NASCE2011
    • Place of Presentation
      Michigan league(Ann Arbor)
    • Year and Date
      2011-07-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Which estrogen receptor (ESR1 ; ERα or EXR2 ; ERβ) is involved in sex determination and gonadal differentiation?2011

    • Author(s)
      Kohno, S.Katus, Y.Ohta, Y.Igutchi, t., Guillette, L.J.
    • Organizer
      NASCE 2011
    • Place of Presentation
      Michigan University Michigan league (Ann Arbor)
    • Year and Date
      2011-07-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] A Preliminary Study on Immunohisitochemical Detection of Estrogen Receptor in American Alligator Oviducts-Specificity of Antibodies2009

    • Author(s)
      Ohta, Y., Urushidani, H., Takeuchi, T., Iguchi, T., Katsu, Y., Kohno, S., Brandon, M. and Guillette, L. J.
    • Place of Presentation
      Tulene University(New Orleans)
    • Year and Date
      2009-10-21
    • Related Report
      2011 Final Research Report
  • [Presentation] A Preliminary Study on Immunohisitochemical Detection of Estrogen Receptor in American Alligator Oviducts-Specificity of Antibodies2009

    • Author(s)
      Ohta, Y., Urushidani, H., Takeuchi, T., Iguchi, T., Katsu, Y., Kohno, S., Brandon, M., Guillette, L.J.
    • Organizer
      e.hormone
    • Place of Presentation
      New Orleans
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://staff.muses.tottori-u.ac.jp/ohta

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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