| Project/Area Number |
21510086
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TSUYOSHI Sugio 元岡山大学, 自然科学研究科, 教授 (20033269)
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| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 鉄酸化細菌 / Acidithiobacillus ferrooxidans / 高度水銀耐性株 / 水銀気化活性 / 酵素解析 / Cytochrome c oxidas / 電気培養 / Bioremediation / ctochrome coxidas / 環境浄化 / 微生物固定化 / Acidithiobacillus ferrooaidans |
|---|
| Research Abstract |
Acidithiobacillus ferrooxidans MON-1 is highly resistant not only to mercuric chloride but also to organomercurials. It have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity, We have found that cytochrome c oxidase, purified from strain MON-1, reduces HgCl_2 to volatilizable metal mercury(Hg^0) with reduced mammalian cyt. c or Fe^<2+> as an electron donor. In this study we found that cyt. c oxidase can volatilize Hg^0 from CH_3HgCl(MMC) as well as from HgCl_2 with reduced mammalian cyt. c or c-type cyt. c oxidase purified from strain MON-1 as an electron donor. We also found that MMC volatilization activity is present in the MON-1 plasma membrane but not in the cytosol. These activities were strongly inhibited by NaCN and the antibody produced against purified MON-1 cyt. c oxidase.
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