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Bacterial heavy metal transporter MerC increases mercury accumulation in Arabidopsis thaliana.

Research Project

Project/Area Number 21510088
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionKitasato University

Principal Investigator

KIYONO Masako  北里大学, 薬学部, 准教授 (30239842)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsメチル水銀トランスポーター / MerE / MerF / メチル水銀浄化 / ファイトレメディエーション / 土壌汚染浄化
Research Abstract

This study evaluated the feasibility of transgenic Arabidopsis engineered to express the bacterial heavy metal transporter MerC for the phytoremediation of mercury pollution. MerC, MerC-SYP121, or MerC-AtVAM3 proteins were found to be expressed in leaf segments of transgenic plants using an anti-MerC antibody immunostaining method. Transgenic Arabidopsis plants expressing merC, merC-SYP121, and merC-AtVAM3 were more resistant to mercury and accumulated significantly more of this metal compared with wild-type Arabidopsis. These results demonstrated that the expression of the bacterial heavy metal transporter MerC can promote the transport and accumulation of mercury in transgenic Arabidopsis, which represents a method of improving plants for the phytoremediation of mercury pollution.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (13 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance2012

    • Author(s)
      Kiyono M, Oka Y, Sone Y, Tanaka M, Nakamura R, Sato MH, Pan-Hou H, Sakabe K, Inoue K
    • Journal Title

      Planta

      Volume: 235(4) Pages: 841-850

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance2012

    • Author(s)
      Masako Kiyono, et al.(9)
    • Journal Title

      Planta

      Volume: 235 Pages: 841-850

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid monitoring of mercury in air from an organic chemical factory in China using a portable mercury analyzer2011

    • Author(s)
      Yasutake A, Cheng JP, Kiyono M, Uraguchi S, Liu X, Miura K, Yasuda Y, Mashyanov N
    • Journal Title

      Scientific World J

      Volume: 11 Pages: 1630-1640

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic expression of bacterial merC fused with plant SNARE in Saccharomyces cerevisiae increased mercury accumulation2011

    • Author(s)
      Kiyono M, Sone Y, Miyahara K, Oka Y, Nakamura M, Nakamura R, Sato MH, Pan-Hou H, Sakabe K, Inoue K
    • Journal Title

      Biochem Eng J

      Volume: 56 Pages: 137-141

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for induced cadmium accmulation2010

    • Author(s)
      Kiyono M, Miyahara K, Sone Y, Pan-Hou H, Uraguchi S, Nakamura R, Sakabe K
    • Journal Title

      Appl Microbiol Biotechnol

      Volume: 86 Pages: 753-759

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a luminescence-based biosensor for detection of methylmercury2010

    • Author(s)
      Nagata T, Muraoka T, Kiyono M, Pan-Hou H
    • Journal Title

      J Toxicol Sci

      Volume: 35 Pages: 231-234

    • NAID

      10026193447

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roles played by MerE and MerT in the transport of inorganic and organic mercury compounds in gram-negative bacteria2010

    • Author(s)
      Sone Y, Pan-Hou H, Nakamura R, Sakabe K, Kiyono M
    • Journal Title

      J Health Sci

      Volume: 56 Pages: 123-127

    • NAID

      130000157713

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation.2010

    • Author(s)
      Masako Kiyono, et al.(6)
    • Journal Title

      Appl Microbiol Biotechnol 86

      Pages: 753-759

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Roles played by MerE and MerT in the transport of inorganic and organic mercury compounds in gram-negative bacteria.2010

    • Author(s)
      Masako Kiyono, et al.(4)
    • Journal Title

      J Health Sci 56

      Pages: 123-127

    • NAID

      130000157713

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a luminescence-based biosensor for detection of methyl mercury.2010

    • Author(s)
      Masako Kiyono, et al.(3)
    • Journal Title

      J Toxicol Sci 35

      Pages: 231-234

    • NAID

      10026193447

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Pseudomonas K-62由来68 kb plasmid(pMR68)の水銀耐性遺伝子に関する研究2011

    • Author(s)
      望月優佑、清野正子、曽根有香、中村亮介、井上健一郎
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡
    • Year and Date
      2011-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] MerCおよびSNAREを利用したカドミウムのファイトレメディエーション2010

    • Author(s)
      清野正子, 曽根有香, 中村亮介, 佐藤雅彦, 坂部貢, 井上健一郎
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      兵庫
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] MerCおよびSNAREを利用したカドミウムのファイトレメディエーション2010

    • Author(s)
      清野正子, ら他
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pseudomonas K-62由来68 kb plasmidの水銀耐性遺伝子に関する研究2010

    • Author(s)
      望月優佑、清野正子、曽根有香、中村亮介、井上健一郎
    • Organizer
      第54回日本薬学会関東支部大会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerEの機能解析およびファイトレメディエーションへの応用2010

    • Author(s)
      曽根有香、清野正子、中村亮介、坂部貢、井上健一郎
    • Organizer
      第19回日本臨床環境医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerFを利用したメチル水銀高蓄積植物の作出2010

    • Author(s)
      清野正子、東條博隆、曽根有香、中村亮介、坂部貢、井上健一郎
    • Organizer
      第19回日本臨床環境医学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerFおよびSNARE分子を利用したメチル水銀高蓄積植物の作出2010

    • Author(s)
      清野正子、東條博隆、曽根有香、中村亮介、芳生秀光、坂部貢
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] MerEの水銀輸送活性を利用した水銀ファイトレメディエーション系の構築2010

    • Author(s)
      曽根有香、中村亮介、芳生秀光、坂部貢、清野正子
    • Organizer
      日本薬学会第130年会
    • Place of Presentation
      岡山
    • Year and Date
      2010-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] Tn21由来MerEのメチル水銀輸送機能の解析フォーラム2009 :衛生薬学・環境トキシコロジー2009

    • Author(s)
      曽根有香、中村亮介、芳生秀光、坂部貢、清野正子
    • Organizer
      日本薬学会環境衛生部会
    • Place of Presentation
      沖縄
    • Year and Date
      2009-11-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerCを高発現した植物の水銀浄化活性フォーラム2009 :衛生薬学・環境トキシコロジー2009

    • Author(s)
      清野正子、曽根有香、東條博隆、中村亮介、芳生秀光、佐藤雅彦、坂部貢
    • Organizer
      日本薬学会環境衛生部会
    • Place of Presentation
      沖縄
    • Year and Date
      2009-11-06
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerFおよびSNARE分子を利用したメチル水銀高蓄積植物の作出2009

    • Author(s)
      東條博隆、曽根有香、中村亮介、芳生秀光、坂部貢、清野正子
    • Organizer
      日本薬学会環境衛生部会
    • Place of Presentation
      沖縄
    • Year and Date
      2009-11-05
    • Related Report
      2011 Final Research Report
  • [Presentation] Tn21由来MerEの水銀輸送に関する研究2009

    • Author(s)
      曽根有香、清野正子、芳生秀光、中村亮介、坂部貢
    • Organizer
      第18回日本臨床環境医学会学術集会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Presentation] 水銀トランスポーターMerC及びシンタキシンSNARE分子を利用した水銀高蓄積植物の作出2009

    • Author(s)
      清野正子、岡由美子、佐藤雅彦、曽根有香、中村亮介、坂部貢
    • Organizer
      第18回日本臨床環境医学会学術集会
    • Place of Presentation
      岡山
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://bukai.pharm.or.jp/bukai_kanei/Topics/Topics17.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 重金属高蓄積性の形質転換体および重金属汚染の浄化法2010

    • Inventor(s)
      清野正子
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Acquisition Date
      2010-04-30
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 重金属高蓄積性の形質転換体および重金属汚染の浄化法2010

    • Inventor(s)
      清野正子
    • Industrial Property Rights Holder
      学校法人北里研究所
    • Acquisition Date
      2010-04-30
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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