Regulation of microbial communities and application to bioremediation in marine environments

• Project/Area Number: 21510090
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Nihon University
• Principal Investigator: IWABUCHI Noriyuki 日本大学, 生物資源科学部, 講師 (90328708)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 環境修復技術 / メタプロテオーム / 海洋石油汚染 / 多環芳香族炭化水素 / バイオレメデーション / Cycloclasticus / ゲノム / PAHs分解酵素

Research Abstract

Cycloclasticus is important for biodegradation of polycyclic aromatic hydrocarbon(PAHs) in seawater and is expected to apply to bioremediation for oil spills for marine environments. In this study, metaproteogenomic analysis of PAHs degrading marine bacterial community was performed. The results suggested that Cycloclasticus group became predominant in the microbial community.

Research Products

• [Journal Article] Bacillus trypoxylicola sp. nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle (2010)
  • Author(s): Aizawa T, Iwabuchi N, et al
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 60 Pages: 61-66
  • Peer Reviewed
• [Journal Article] 海洋性多環芳香族炭化水素(PAHs)分解菌Cycloclasticus培養制御技術の開発 (2010)
  • Author(s): 竹石英伯, 岩淵範之, 他4名
  • Journal Title: 廃水と用水 Volume: 52(5) Pages: 359-364
• [Journal Article] 生物膜成分による石油汚染海洋の浄化-細胞外多糖の性質と機能およびそれを利用した微生物制御技術- (2010)
  • Author(s): 岩淵範之
  • Journal Title: 水環境学会誌 Volume: 33(4) Pages: 121-125
  • NAID: 10026288978
• [Journal Article] Bacillus trypoxylicola sp.nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle (2010)
  • Author(s): Aizawa, Iwabuchi, et al
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Volume: 60 Pages: 61-66
  • Peer Reviewed
• [Journal Article] Bacillus trypoxylicola sp.nov., a xylanase-producing alkaliphilic bacterium isolated from larval gut of Trypoxylus dichotomus, the Japanese horned beetle (2010)
  • Author(s): 岩淵範之, et al.
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 60 Pages: 61-66
  • Peer Reviewed
• [Journal Article] Role of Interfacial Tension in the Translocation of Rhodococcus erythropolis during Growth in a Two-Phase Culture System (2009)
  • Author(s): Iwabuchi N, et al
  • Journal Title: Environmental Science & Technology Volume: 49 Pages: 8290-8294
  • Peer Reviewed
• [Journal Article] 油-微生物の相互作用における細胞外多糖(extracellular polysaccharide, EPS)の影響-EPSを用いた海洋石油汚染の浄化を中心に- (2009)
  • Author(s): 岩淵範之
  • Journal Title: Bacterial Adherence & Biofilm Volume: 23 Pages: 13-18
• [Journal Article] Role of Interfacial Tension in the Translocation of Rhodococcus erythropolis during Growth in a Two-Phase Culture System (2009)
  • Author(s): 岩淵範之, et al.
  • Journal Title: Environmental Science & Technology 49 Pages: 8290-8294
  • Peer Reviewed
• [Journal Article] 油―微生物の相互作用における細胞外多糖(extracellular polysaccharide, EPS)の影響―EPSを用いた海洋石油汚染の浄化を中心に― (2009)
  • Author(s): 岩淵範之
  • Journal Title: Bacterial Adherence & Biofilm 23 Pages: 13-18
• [Presentation] 石油汚染下の微生物群集中における海洋性多環芳香族炭化水素(PAHs)分解菌Cycloclasticusの培養制御について (2012)
  • Author(s): 山下、岩淵, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Rhodococcus erythropolis PR4株の培地/アルカン二相培養系における細胞の局在性に対するgroES-EL1遺伝子の影響 (2012)
  • Author(s): 瀧原、岩淵, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Rhodococcus rhodochrousの培地/アルカン二相培養系における細胞の局在性へのgroEL2遺伝子の影響 (2012)
  • Author(s): 松浦、岩淵, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] シリングアルデヒドから水溶性の黒色色素を生産する微生物Pseudomonas sp.ITH-SA-1 (2012)
  • Author(s): 滝口、岩淵, 他
  • Organizer: 日本農芸化学会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] Rhodococcus erythropolis PR4株の培地/アルカン二相培養系における細胞の局在性に対するgroES-EL1遺伝子の影響 (2011)
  • Author(s): 瀧原、岩淵, 他
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-10-08
• [Presentation] 油-微生物の相互作用における細胞外多糖(EPS)の影響 (2009)
  • Author(s): 岩淵範之
  • Organizer: Bacterial Adherence & Biofilm 第23回学術集会
  • Place of Presentation: 第一三共
  • Year and Date: 2009-07-11
• [Patent(Industrial Property Rights)] 形質転換されたロドコッカス(Rhodococcus)属細菌、及び、これを用いた炭化水素の処理方法 (2009)
  • Inventor(s): 岩淵, 他
  • Industrial Property Rights Holder: 日本大学
  • Filing Date: 2009-09-07
• [Patent(Industrial Property Rights)] 形質転換されたロドコッカス(Rhodococcus)属細菌、及び、これを用いた炭化水素の処理方法 (2009)
  • Inventor(s): 岩淵範之, 他
  • Industrial Property Rights Holder: 日本大学
  • Filing Date: 2009-09-09