Analyses on Diversity and Functions of Anaerobic Acetate Metabolizing Syntrophic Microorganisms by Using a Liquid Separation and Cultivation Technique.

• Project/Area Number: 21510092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: SHIGEMATSU Toru 新潟薬科大学, 応用生命科学部, 准教授 (10315286)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 環境負荷低減技術 / 嫌気性微生物群集 / メタン発酵 / 難培養性微生物 / 分離培養法 / 酢酸代謝微生物共生系 / 嫌気性生物群集

Research Abstract

A high-throughput separation and cultivation technique of anaerobic acetate-metabolizing microorganisms, which contain aceticlastic methanogens and microbial syntroph of acetate-oxidizing bacteria and hydrogenotrophic methanogens, was developed by liquid cultivation using microplates. By PCR detection of Archaea in the wells of microplates after cultivation, distribution of archaeal cells was proven to be agreed with Poisson's distribution. The separation and cultivation of anaerobic acetate-metabolizing microorganisms could be successfully carried out using this new technique.

Research Products

• [Journal Article] (2010)
  • Author(s): 重松亨(分担執筆)
  • Journal Title: 難培養微生物研究の最新技術II(シーエムシー出版) Pages: 241-247
• [Journal Article] メタン発酵プロセスに関与する微生物群集 (2009)
  • Author(s): 重松亨、湯岳琴、木田建次
  • Journal Title: 生物工学会誌 Volume: 87(12) Pages: 570-596
  • NAID: 110007505180
• [Journal Article] メタン発酵プロセスに関与する微生物群集 (2009)
  • Author(s): 重松亨(共著)
  • Journal Title: 生物工学会誌 87巻 Pages: 570-596
  • NAID: 110007505180
• [Presentation] メタン発酵槽に生息する微生物群集の構造と機能 (2012)
  • Author(s): 重松亨
  • Organizer: バイオマス & エネルギーexpo in新潟
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2012-03-01
• [Presentation] メタン発酵槽に生息する微生物群集の構造と機能 (2012)
  • Author(s): 重松亨
  • Organizer: バイオマス&エネルギーexpo in新潟
  • Place of Presentation: 朱鷺メッセ
  • Year and Date: 2012-03-01
• [Presentation] メタン発酵プロセスにおいて酢酸を分解する微生物群集の構造および機能 (2011)
  • Author(s): 重松亨
  • Organizer: 第3回メタン高度利用技術シンポジウム
  • Place of Presentation: 長岡商工会議所
  • Year and Date: 2011-12-05
• [Presentation] 無加温実下水処理UASBリアクターの微生物群集構造と高頻度に検出される未培養微生物群の検出 (2011)
  • Author(s): 井口晃徳、重松亨、大久保努、大矢明子、高橋優信、久保田健吾、関口勇地、荒木信夫、山口隆司、原田秀樹
  • Organizer: 第3回メタン高度利用技術シンポジウム
  • Place of Presentation: 長岡商工会議所
  • Year and Date: 2011-12-05
• [Presentation] マイクロプレートを用いた嫌気性酢酸利用メタン生成微生物群の分離培養系の構築 (2011)
  • Author(s): 重松亨, 中島美沙子, 林真由美, 上野茂昭, 藤井智幸, 正木春彦, 井口晃徳, 平山匡男
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-26
• [Presentation] マイクロプレートを用いた嫌気性酢酸代謝微生物共生系の分離培養 (2010)
  • Author(s): 中島美沙子, 林真由美, 上野茂昭, 藤井智幸, 正木春彦, 重松亨, 平山匡男
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
• [Presentation] A culture-dependent bacterial community structure analysis based on liquid cultivation and its application to a marine environment (2009)
  • Author(s): Toru Shigematsu, Mayumi Hayashi, Isamu Kikuchi, Shigeaki Ueno, Haruhiko Masaki and Tomoyuki Fujii
  • Organizer: Asia Pacific Biochemical Engineering Conference
  • Place of Presentation: Kobe International Conference Center
  • Year and Date: 2009-11-25
• [Presentation] A culture-dependent bacterial community structure analysis based on liquid cultivation and its application to a marine environment (2009)
  • Author(s): Toru Shigematsu(共著)
  • Organizer: Asia Pacific Biochemical Engineering Conference 2009
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-11-25
• [Book] 食品・環境と難培養性微生物(難培養微生物研究の最新技術II) (2010)
  • Author(s): 重松亨, 大熊盛也, 工藤俊章
  • Publisher: シーエムシー出版