| Project/Area Number |
21510095
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Environmental technology/Environmental materials
|
|---|
| Research Institution | Waseda University (2011)
Fukuyama University (2009-2010) |
|---|
Principal Investigator |
OHKAWA Hideo 早稲田大学, 先端科学・健康医療融合研究機構, 研究員 (90233048)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHTA Masaya 福山大学, 生命工学部, 教授 (00203802)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TAKEYAMA Haroko 早稲田大学, 理工学術院先進理工学部, 教授 (60262234)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 環境分析 / 有害化学物質 / 遺伝子組換え植物 / バイオサーファクタント / バイオモニタリング / ダイオキシン / PCB / アリルハイドロカーボン受容体 / 免疫化学測定方法 / モノクローナル抗体 / 遺伝子組換え抗体 / PCB同族体 / 組換え体シロイヌナズナ / モルモットAhR / IAA / カドミウム / キャリアー蛋白質 / アッセイ / AhRリガンド / GUSレポーター |
|---|
| Research Abstract |
It was attempted to develop convenien polychlorinated biphenyls(PCBs) based on molecular mechanisms of biological functions in mammals. (1) Recombinant aryl hydrocarbon receptor(AhR)-mediatedβ-glucuronidase(GUS) reporter gene expression systems were each introduced into tobacco t biochemical assays of and Arabidopsis plants. The transgenic plants were developed for GUS assays of PCBs. Particularly, the use of biosurfactants improved GUS assay sensitivity of PCBs in the transgenic plants. (2) Monoclonal and recombinant antibodies specific to PCBs were prepared for development of Enzyme-Linked Immunosorbent Assays(ELISAs) of PCBs. The ELISAs selectively detected coplanar PCBs.
|
