Structure-function studies of enzymes for design of novel biopolyesters

• Project/Area Number: 21510102
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: HISANO Tamao 独立行政法人理化学研究所, 城生体金属科学研究室, 専任研究員 (20312267)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: バイオポリエステル / 生分解性 / 微生物酵素 / 結晶構造解析 / 構造と機能 / 酵素 / 立体構造

Research Abstract

Polyhydroxyalkanoate(PHA) is an important bacterial polyester that is degradable in environment. For establishment of a design and synthetic system of a novel PHA from aromatic components of biomass, crystal structures of PHA depolymerase and phenylacetic acid degradation protein PaaG were determined.

Research Products

• [Journal Article] Crystal structure of phenylacetic acid degradation protein PaaG from Thermus thermophilus HB8 (2009)
  • Author(s): Tomoyasu Kichise, Tamao Hisano, Kazuki Takeda, Kunio Miki
  • Journal Title: PROTEINS : Structure, Function, and Bioinformatics Volume: 76 Pages: 779-786
  • Peer Reviewed
• [Journal Article] Crystal structure of phenylacetic acid degradation protein PaaG from Thermus thermophilus HB8 (2009)
  • Author(s): Tomoyasu Kichise, Tamao Hisano, Kazuki Takeda, Kunio Miki
  • Journal Title: PROTEINS : Structure, Function, and Bioinformatics 76 Pages: 779-786
  • Peer Reviewed
• [Presentation] Crystal structures of catalytic domains of bacterial and fungal PHB depolymeases (2011)
  • Author(s): Tamao Hisano
  • Organizer: The 3rd International Conference on Bio-based Polymers
  • Place of Presentation: Beijing
  • Year and Date: 2011-10-21
• [Presentation] Crystal structures of catalytic domains of bacterial and fungal PHB depolymeases (2011)
  • Author(s): Tamao Hisano
  • Organizer: The 3rd International Conference on Bio-based Polymers
  • Place of Presentation: Beijing, China(招待講演)
  • Year and Date: 2011-10-21
• [Presentation] Crystal Structure of the Catalytic Domain of Multidomain PHB Depolymerase (2011)
  • Author(s): Tamao Hisano, Mari Shiraki, Yoshitsugu Shiro, Kunio Miki, Terumi Saito
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Madrid
• [Presentation] Crystal Structure of the Catalytic Domain of Multidomain PHB Depolymerase (2011)
  • Author(s): Tamao Hisano, Mari Shiraki, Yoshitsugu Shiro, Kunio Miki, Terumi Saito
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Madrid, Spain
• [Presentation] フェニル酢酸分解に関与する酵素PaaGの結晶構造 (2010)
  • Author(s): 吉瀬智康、久野玉雄、竹田一旗、三木邦夫
  • Organizer: 第23回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: 姫路
  • Year and Date: 2010-01-08
• [Presentation] フェニル酢酸分解に関与する酵素PaaGの結晶構造 (2010)
  • Author(s): 吉瀬智康、久野玉雄、竹田一旗、三木邦夫
  • Organizer: 第23回日本放射光学会年会・放射光科学合同シンポジウム
  • Place of Presentation: イーグレひめじ(姫路市)
  • Year and Date: 2010-01-08
• [Presentation] フェニル酢酸分解系酵素PaaGの結晶構造 (2009)
  • Author(s): 吉瀬智康、久野玉雄、竹田一旗、三木邦夫
  • Organizer: 日本結晶学会2009年度年会
  • Place of Presentation: 西宮
• [Presentation] フェニル酢酸分解系酵素PaaGの結晶構造 (2009)
  • Author(s): 吉瀬智康、久野玉雄、竹田一旗、三木邦夫
  • Organizer: 日本結晶学会2009年度年会
  • Place of Presentation: 関西学院大学西宮上ヶ原キャンパス