| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
To elucidate the mechanisms of intermittent blinking kinetics, we developed a series of novel data-driven time series analysis and modeling techniques to extract the underlying kinetic scheme directly from single molecule (SM) quantum dot (QD) fluorescence experiments. These include the development and application of the change-point analysis that allows us to correctly extract the on and off intensity dwell-time statistics, and the development of a novel kinetic scheme (KS) construction methodology which unveils the most unbiased KS capturing the full statistical information from time series. The developed methods have demonstrated their high reliability and applicability not only in SM QD experiments, but also in a wide range of other SM measurements, such as in the SM enzymatic turnover experiments and the SM F1_ATPase rotation assessments.
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