Mechanism of U1 snRNP-independent splicing
Project/Area Number |
21510204
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
基礎ゲノム科学
|
Research Institution | Kobe University |
Principal Investigator |
INOUE Kunio 神戸大学, 大学院・理学研究科, 教授 (40252415)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | ゲノム発現 / スプライシング / イントロン / スプライス部位 / U1 snRNP / 熱ストレス / エキソン |
Research Abstract |
Many eukaryotic genes are interrupted by introns that are removed from primary transcripts by RNA splicing. U1 snRNP plays a crucial role in the 5' splice site recognition during splicing. Recently, we found the first example of naturally-occurring U1-independent U2-type splicing in humans. Our present study showed that a tissue-specific splicing regulator, Fox-1, failed to induce hF1γexon 9 skipping if the 5' splice site of intron 9 was mutated to the U1-dependent type, suggesting that U1-independent splicing contributes to the regulation of alternative splicing of a class of pre-mRNAs.
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Report
(4 results)
Research Products
(14 results)